Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China

Mårtensson, Lotta; Dı́ez, Beatriz; Wartiainen, Ingvild; Zheng, Weiran; El-Shehawy, Rehab; Rasmussen, Ulla

doi:10.1007/s11104-009-9970-8

Cited by 71 publications

(42 citation statements)

References 47 publications

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“…PCR amplifications of the nifH gene were performed using the universal primers PolF/PolR (Poly et al, 2001) that cover most of the known diazotrophic organisms (Bacteria and Archaea), including cyanobacteria (Mårtensson et al, 2009;Díez et al, 2012). These primers amplify fragments 360 bp in length.…”

Section: Bacterial Nifh Gene Clone Librarymentioning

confidence: 99%

The cyanobacterium Mastigocladus fulfills the nitrogen demand of a terrestrial hot spring microbial mat

et al. 2015

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Research (CR)2, Santiago, ChileCyanobacteria from Subsection V (Stigonematales) are important components of microbial mats in non-acidic terrestrial hot springs. Despite their diazotrophic nature (N 2 fixers), their impact on the nitrogen cycle in such extreme ecosystems remains unknown. Here, we surveyed the identity and activity of diazotrophic cyanobacteria in the neutral hot spring of Porcelana (Northern Patagonia, Chile) during 2009 and 2011-2013. We used 16S rRNA and the nifH gene to analyze the distribution and diversity of diazotrophic cyanobacteria. Our results demonstrate the dominance of the heterocystous genus Mastigocladus (Stigonematales) along the entire temperature gradient of the hot spring (69-38°C). In situ nitrogenase activity (acetylene reduction), nitrogen fixation rates (cellular uptake of 15 N 2 ) and nifH transcription levels in the microbial mats showed that nitrogen fixation and nifH mRNA expression were light-dependent. Nitrogen fixation activities were detected at temperatures ranging from 58°C to 46°C, with maximum daily rates of 600 nmol C 2 H 4 cm − 2 per day and 94.1 nmol N cm − 2 per day. These activity patterns strongly suggest a heterocystous cyanobacterial origin and reveal a correlation between nitrogenase activity and nifH gene expression during diurnal cycles in thermal microbial mats. N and C fixation in the mats contributed~3 g N m − 2 per year and 27 g C m − 2 per year, suggesting that these vital demands are fully met by the diazotrophic and photoautotrophic capacities of the cyanobacteria in the Porcelana hot spring.

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Section: Bacterial Nifh Gene Clone Librarymentioning

confidence: 99%

The cyanobacterium Mastigocladus fulfills the nitrogen demand of a terrestrial hot spring microbial mat

et al. 2015

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“…Therefore, we used a culture-independent approach, based on PCR amplification and sequencing of nifH DNA (18,19), to assess the diversity of potential diazotrophs associated with switchgrass. nifH has been used by many researchers to study total and functional diversity of bacteria in various environments (15,(20)(21)(22), and phylogenies based on nifH and 16S rRNA generally are congruent (23,24). Additionally, PCR amplification of cDNA derived from nifH RNA was used to determine whether any bacteria express this gene in association with switchgrass.…”

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confidence: 99%

Diversity of Nitrogen-Fixing Bacteria Associated with Switchgrass in the Native Tallgrass Prairie of Northern Oklahoma

Bahulikar

Torres‐Jerez

Worley

et al. 2014

Appl Environ Microbiol

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Switchgrass (Panicum virgatum L.) is a perennial C 4 grass native to North America that is being developed as a feedstock for cellulosic ethanol production. Industrial nitrogen fertilizers enhance switchgrass biomass production but add to production and environmental costs. A potential sustainable alternative source of nitrogen is biological nitrogen fixation. As a step in this direction, we studied the diversity of nitrogen-fixing bacteria (NFB) associated with native switchgrass plants from the tallgrass prairie of northern Oklahoma (United States), using a culture-independent approach. DNA sequences from the nitrogenase structural gene, nifH, revealed over 20 putative diazotrophs from the alpha-, beta-, delta-, and gammaproteobacteria and the firmicutes associated with roots and shoots of switchgrass. Alphaproteobacteria, especially rhizobia, predominated. Sequences derived from nifH RNA indicated expression of this gene in several bacteria of the alpha-, beta-, delta-, and gammaproteobacterial groups associated with roots. Prominent among these were Rhizobium and Methylobacterium species of the alphaproteobacteria, Burkholderia and Azoarcus species of the betaproteobacteria, and Desulfuromonas and Geobacter species of the deltaproteobacteria.

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“…The former occurs as an intracellular endosymbiont in the gills of marine bivalves which provide host with enzyme including cellulases and nitrogenase critical for nitrogen deficiency [2,18,44,84,85]. While the latter, Azotobacter, is a free living diazotrophic bacteria with metabolic capabilities including atmospheric nitrogen fixation by conversion to ammonia and have a unique system of distinct nitrogenase enzyme [2,35,36,37,44,54].…”

Section: Gammaproteobacteria Groups Is Closely Affiliated Withmentioning

confidence: 99%

“…The diversity of cyanobacterial mats inhabiting different environments have been the focus of numerous studies on the several places such as: Tikehau atoll-French Polynasia [1,66], in New Caledonia [8,9], and in the Western Indian Ocean in Zanzibar-Tanzania [4]. These studies identified different structures which differs in appearance [56], species composition, mode of growth and affiliation to the benthic organism including the coral and their substrate. Thus, these types of bacteria are vital during calcification and decalcification process [8,9], much, in the stability of coralalgae symbosis [32,33,63,76,77].…”

Section: Gammaproteobacteria Groups Is Closely Affiliated Withmentioning

confidence: 99%

“…Furthermore, a number of nifH sequences were detected to be affiliated with Xanthobacter, a nutritionally versatile bacterium, with an unprecedented array of metabolic capability relevant in nitrogen fixation [52,54]. Although the magnitude of transfer of fix nitrogen from diazotrophs into other compartments of the coral holobiont (e.g Symbiodinium) has not been quantified, recent studies shows that nitrogen fixation is a highly energy consuming process which requires 16 mol of ATP for the reduction of 1 mol of dinitrogen [54,65].…”

Section: Gammaproteobacteria Groups Is Closely Affiliated Withmentioning

confidence: 99%

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Response of the Coral Associated Nitrogen Fixing Bacteria Toward Elevated Water Temperature

Diaz¹

2017

WROS

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Abstract:Coral reefs are among the most biologically diverse and economically important ecosystem on the planet. Despite the importance, reef habitat is being under threat from human exploitation, and its most serious stressor is increasing seawater temperature, an aftermath of global warming phenomenon. The increasing seawater temperature causes bleaching, diseases and insufficiency of nutrients of corals. Despite being surrounded by ocean waters were nutrient are in short supply, the reef ecosystem is a significant source of new nitrogen. Biological nitrogen fixation is a significant internal source of marine organism. The growth of all organism lies on the availability of mineral nutrients particularly of nitrogen (N 2 ). Approximately 80% of atmosphere is made of nitrogen, however, N 2 can only be available for use by organism unless it undergoes a process of nitrogen fixation. In this aspect, related literature on biological nitrogen fixation seems sparse especially on the effects of increasing seawater temperature, a well-known contributing factor of coral bleaching. In this study, an investigation was conducted on nitrogen fixing bacterial communities associated in the coral Acropora digitifera, exploring its responses towards elevated water temperature. The study shows that exposure to high temperature causes a drastic change in the community of nitrogen fixing bacteria which are abundant in coral mucus. These changes, is correlated with the shift in the metabolic function in coral holobiont, thus, affecting both health and resiliency of corals. Overall, the finding highlights the impact of elevated seawater temperature on the nitrogen fixing bacterial composition and its diversity as well as its effects of this on host metabolism.

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Diazotrophic diversity, nifH gene expression and nitrogenase activity in a rice paddy field in Fujian, China

Cited by 71 publications

References 47 publications

The cyanobacterium Mastigocladus fulfills the nitrogen demand of a terrestrial hot spring microbial mat

The cyanobacterium Mastigocladus fulfills the nitrogen demand of a terrestrial hot spring microbial mat

Diversity of Nitrogen-Fixing Bacteria Associated with Switchgrass in the Native Tallgrass Prairie of Northern Oklahoma

Response of the Coral Associated Nitrogen Fixing Bacteria Toward Elevated Water Temperature

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