Dibromoethane effects on the induction of ?-glutamyl-transpeptidase positive foci in rat liver

Milks, Michael M.; Wilt, Stephen R.; Ali, Ibrahim; Pereira, Michael A.; Couri, Daniel

doi:10.1007/bf00279318

Cited by 16 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In each experiment the mean number of foci/cm2 for the DEN + PB positive control groups was on the low side of the range of values reported for the Sprague-Dawley rat in studies using similar protocols (Pereira et al , 1982;Milks et al , 1983b). The only difference in procedures, apart from animal strains, was the administration of PB in rat chow rather than in the drinking water.…”

Section: Resultsmentioning

confidence: 73%

See 1 more Smart Citation

Differences in Rat Liver Enzyme-Altered Foci Produced By Chlorinated Aliphatics and Phenobarbital

et al. 1986

View full text Add to dashboard Cite

Nine chlorinated aliphatics (CAs)--1,1-dichloroethane, 1,2-dichloroethane, 1,1,1-trichloroethane, 1,1,2-trichloroethane, trichloroethylene, tetrachloroethylene, 1,1,1,2-tetrachloroethane, 1,1,2,2-tetrachloroethane, and hexachloroethane--were examined in a rat liver foci assay for evidence of initiating and promoting potential. Young adult male Osborne-Mendel rats (ten/group) were given partial hepatectomies, followed 24 hr later by a single i.p. dose of either diethylnitrosamine (30 mg/kg body weight) or CA, 1 wk later either a diet containing 0.05% (w/w) phenobarbital or daily oral gavage (5 X/wk) of CA in corn oil for 7 weeks, and sacrificed 1 wk later. Putative preneoplastic markers monitored were foci with increased gamma-glutamyltranspeptidase activity [GGT(+)]. CAs were without significant effect in the initiation protocol at the maximum tolerated dose. In the promotion protocol, 1,1-dichloroethane, 1,1,2-trichloroethane, tetrachloroethylene, 1,1,2,2-tetrachloroethane, and hexachloroethane induced significant increases in GGT(+) foci above control levels. Two variants of GGT(+) foci were distinguishable, one associated predominantly with phenobarbital promotion, resembling preneoplastic foci in other models, and the other associated with CA promotion, which was less intensely stained and exhibited branching, resembling foci undergoing redifferentiation. The marked differences in response may relate to differences in cytotoxic potential or mechanism of action of the two types of agents.

show abstract

Section: Resultsmentioning

confidence: 73%

“…, 1978;Tsuda et al . Trichloroethylene (TCY), however, was without effect (Milks et al , 1983b), possibly because the doses administered were well below the maximum that can be tolerated (Mitoma et al, 1985). , , 1984Shinozuka and Lombardi, 1980;Pereira et al .…”

mentioning

confidence: 99%

Differences in Rat Liver Enzyme-Altered Foci Produced By Chlorinated Aliphatics and Phenobarbital

et al. 1986

View full text Add to dashboard Cite

show abstract

“…A major reason why an initiating effect of DBE was evident in this study but not in the study of Milks et al (1982) is probably their use of phenobarbital promotion versus our use of AAF-CC14. In a comparison of the efficacy of different short-term systems for enhancing development of liver GGT foci after diethylnitrosamine, Leonard et al (1982) found that phenobarbital promotion produced three times fewer GGT foci in 8 weeks than did AAF-CC14 in 5 weeks.…”

Section: Resultsmentioning

confidence: 94%

“…An apparent exception, according to Milks et al (1982), is the widely used fumigant 1,2-dibromoethane (DBE). They reported that DBE lacked an initiator effect for the livers of Sprague-Dawley rats as assessed by gamma-glutamyl transpeptidase (GGT) positive foci.…”

Section: Introductionmentioning

confidence: 99%

1,2-Dibromoethane initiation of hepatic nodules in Sprague-Dawley rats selected with Solt-Farber system

1985

View full text Add to dashboard Cite

Initiating effects of the fumigant 1,2-dibromoethane (DBE) for liver were examined using the Solt-Farber selection system. Male Sprague Dawley rats (230-260 g) were given one oral dose of DBE (75 mg/kg), a two-thirds partial hepatectomy 4 h later, five oral doses of 2-acetylamidofluorene (AAF) (25 mg/kg) on days 17-21, CCl4 (2 ml/kg) on day 22, a booster dose of AAF (10 mg/kg) on day 32, and were sacrificed on day 82. Nodules and appreciable gamma-glutamyl transpeptidase foci were found in the livers of four of six animals given DBE, but not in any animals of the control group.

show abstract

“…EDB has been shown to be mutagenic in various test systems (Vogel and Chandler 1974;Brem et al 1974;Nachtomi and Sarma 1977;Rannug 1980) and is reported to be carcinogenic to experimental animals and shown to cause squamous cell carcinoma of the stomach in rats and mice (Olson et al 1973;Powers et al 1975). Based on a histochemical bioassay of induction of gamma glutamyl transpeptidase foci in the rat liver, Milks et al (1982) concluded that EDB is a promoter of hepatocarcinogenesis. Other reported effects of EDB are on the reproductive system.…”

Section: Introductionmentioning

confidence: 99%

Reproductive Potential of Male Rats Fed Dietary Ethylene Dibromide

Shivanandappa

Krishnakumari

Majumder

1987

Journal of Food Safety

View full text Add to dashboard Cite

Ethylene dibromide (EDB), a grain fumigant, has been assessed for its toxic effects when fed to growing albino rats at 50, 100, 250 and 500 ppm in the diet for 90 days. Weekly body weight gain showed no effect on growth. Organ weights were normal and no histopathological lesions were observed in the liver, kidney, lungs, brain, spleen, adrenal gland, and the testis. Activities of the serum enzymes, viz., GOT, GPT, LDH and alkaline phosphatase were not affected. EDB fed male rats when mated with normal female rats showed no impairment in their reproductive performance.

show abstract

Dibromoethane effects on the induction of ?-glutamyl-transpeptidase positive foci in rat liver

Cited by 16 publications

References 25 publications

Differences in Rat Liver Enzyme-Altered Foci Produced By Chlorinated Aliphatics and Phenobarbital

Differences in Rat Liver Enzyme-Altered Foci Produced By Chlorinated Aliphatics and Phenobarbital

1,2-Dibromoethane initiation of hepatic nodules in Sprague-Dawley rats selected with Solt-Farber system

Reproductive Potential of Male Rats Fed Dietary Ethylene Dibromide

Contact Info

Product

Resources

About