Dicer Knockdown Inhibits Endothelial Cell Tumor Growth via MicroRNA 21a-3p Targeting of Nox-4

Gordillo, Gayle M.; Biswas, Ayan; Khanna, Savita; Pan, Xueliang; Sinha, Mithun; Roy, Sashwati; Sen, Chandan K.

doi:10.1074/jbc.m113.519264

Cited by 41 publications

(33 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NA934V, lot 9583369, Amersham Biosciences) and antimouse-IgG (1:2000, catalog no. NA931V, lot 6652622, Amersham Biosciences) (8,37) and the enhanced chemiluminescence assay (Amersham Biosciences) according to the manufacturer's instructions. Pixel densitometry for individual bands was done using ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

“…Whole cell lysate was collected using lysis buffer (150 mM KCl, 25 mM Tris-HCl, 5 mM EDTA, 0.5% IgePal, 1 mM PMSF, 1ϫ protease inhibitor) as described previously (8,37). Protein concentration was determined using the Coomassie Plus Assay (Thermo Scientific).…”

Section: Measurement Of Protein Carbonyl Levels and Lipidmentioning

confidence: 99%

“…Simple Western analysis is carried out at room temperature, and instrument default settings were used as discussed earlier (37). MRP-1 (1:50 dilution), p84 (1:50 dilution), and ␤-actin (1:100 dilution) primary antibody were diluted with antibody diluent (ProteinSimple).…”

Section: Measurement Of Protein Carbonyl Levels and Lipidmentioning

confidence: 99%

“…The beads were then washed three times with ice-cold lysis buffer (centrifugation at 2500 ϫ g at 4°C for 5 min). For Western blotting analysis, samples were subjected to SDS-PAGE after reduction with 1 M DTT as described previously (8,37).…”

Section: Measurement Of Protein Carbonyl Levels and Lipidmentioning

confidence: 99%

See 3 more Smart Citations

Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells

Biswas

Khanna

Spieldenner

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Protein Carbonyl Levels and Lipidmentioning

confidence: 99%

Section: Measurement Of Protein Carbonyl Levels and Lipidmentioning

confidence: 99%

Section: Measurement Of Protein Carbonyl Levels and Lipidmentioning

confidence: 99%

See 2 more Smart Citations

Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells

Biswas

Khanna

Spieldenner

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

“…For detection of mRNA, total RNA from cells was extracted using miRVana miRNA isolation kit according to the manufacturer's protocol (Ambion, Life Technologies, Grand Island, NY). For mRNA expression studies, cDNA synthesis was achieved by a SuperScript III first-strand synthesis system (Applied Biosystems, Life Technologies) as described (16). The abundance of mRNA for genes of interest was quantified by using real-time polymerase chain reaction (PCR) with double-stranded DNA binding dye SYBR green-I.…”

Section: Methodsmentioning

confidence: 99%

Endothelial cell tumor growth is Ape/ref-1 dependent

Biswas

Khanna

Roy

et al. 2015

American Journal of Physiology-Cell Physiology

Self Cite

View full text Add to dashboard Cite

forming endothelial cells have highly elevated levels of Nox-4 that release H2O2 into the nucleus, which is generally not compatible with cell survival. We sought to identify compensatory mechanisms that enable tumorforming endothelial cells to survive and proliferate under these conditions. Ape-1/ref-1 (Apex-1) is a multifunctional protein that promotes DNA binding of redox-sensitive transcription factors, such as AP-1, and repairs oxidative DNA damage. A validated mouse endothelial cell (EOMA) tumor model was used to demonstrate that Nox-4-derived H 2O2 causes DNA oxidation that induces Apex-1 expression. Apex-1 functions as a chaperone to keep transcription factors in a reduced state. In EOMA cells Apex-1 enables AP-1 binding to the monocyte chemoattractant protein-1 (mcp-1) promoter and expression of that protein is required for endothelial cell tumor formation. Intraperitoneal injection of the small molecule inhibitor E3330, which specifically targets Apex-1 redox-sensitive functions, resulted in a 50% decrease in tumor volume compared with mice injected with vehicle control (n ϭ 6 per group), indicating that endothelial cell tumor proliferation is dependent on Apex-1 expression. These are the first reported results to establish Nox-4 induction of Apex-1 as a mechanism promoting endothelial cell tumor formation.

show abstract

Post‐ harvest consideration factors for microRNA research in cellular, tissue, serum and plasma samples

Chiang

2014

Cell Biology International

View full text Add to dashboard Cite

MicroRNA plays important regulatory roles on the genetic network which provides researchers with valuable novel mechanistic directions and perspectives for applications in a wide variety of fields. Despite pertinent insight gained from messenger RNA, microRNAs possess distinct biochemical properties that emphasize the need for independent research to be conducted that addresses specifically microRNA handling. This review provides a brief summary on the various factors at post-sample harvesting stages with focus on microRNA from cellular, tissue, plasma and serum samples. It incorporates an up-to-date discussion on the isolation methods, quality control, profiling methods and other post-harvesting factors in microRNA research.

show abstract

Dicer Knockdown Inhibits Endothelial Cell Tumor Growth via MicroRNA 21a-3p Targeting of Nox-4

Cited by 41 publications

References 52 publications

Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells

Multidrug Resistance-associated Protein-1 (MRP-1)-dependent Glutathione Disulfide (GSSG) Efflux as a Critical Survival Factor for Oxidant-enriched Tumorigenic Endothelial Cells

Endothelial cell tumor growth is Ape/ref-1 dependent

Post‐ harvest consideration factors for microRNA research in cellular, tissue, serum and plasma samples

Contact Info

Product

Resources

About