Dicistroviruses

Bonning, Bryony C.; Miller, W. Allen

doi:10.1146/annurev-ento-112408-085457

Cited by 169 publications

(159 citation statements)

References 152 publications

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“…The 5¢-UTR IRES from HCV was of moderate size (324 nt) and activity. 65 In contrast, the smaller IGR IRES elements from the insect viruses IAPV and CrPV have evolved to function in a bicistronic manner (as opposed to the 5¢-UTR), 66,67 similar to the design of our reporter virus technology concept. In addition, these were the smallest of the elements (219 and 190 nt, respectively) tested, and we predicted that they would have the least negative impact on reporter gene retention and stability.…”

Section: Derivation Of Novel Lucrires Reporter Virusesmentioning

confidence: 99%

Optimized ReplicatingRenillaLuciferase Reporter HIV-1 Utilizing Novel Internal Ribosome Entry Site Elements for Native Nef Expression and Function

Alberti

JonesJennifer

MigliettaRiccardo

et al. 2015

AIDS Research and Human Retroviruses

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We previously developed replication-competent reporter HIV-1 (referred to herein as LucR.T2A reporter viruses), utilizing a ''ribosome skipping'' T2A peptide strategy to link Renilla luciferase (LucR) with Nef expression. The demonstrated utility for HIV-1 vaccine and transmission study applications included measurement of neutralizing antibody (NAb) activity in vaccine sera, improved cell-mediated virus inhibition assays, such as T cell-mediated virus inhibition and antibody-dependent cell-mediated cytotoxicity (ADCC) assays, and humanized mouse models. Herein, we extend our prior work and introduce reporter virus technology for applications that require fully functional Nef. We demonstrate that in CD4 +

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Section: Derivation Of Novel Lucrires Reporter Virusesmentioning

confidence: 99%

Optimized ReplicatingRenillaLuciferase Reporter HIV-1 Utilizing Novel Internal Ribosome Entry Site Elements for Native Nef Expression and Function

Alberti

JonesJennifer

MigliettaRiccardo

et al. 2015

AIDS Research and Human Retroviruses

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“…The 5 and intergenic UTRs of dicistroviruses characteristically contain IRES regions that direct translation independent of a 7-methyl guanosine cap [50,51]. SINV-1 has been shown to possess a Type II intergenic IRES based on sequence, structure, and homology within the dicistroviruses [44,52].…”

Section: Solenopsis Invicta Virusmentioning

confidence: 99%

Positive-Strand RNA Viruses Infecting the Red Imported Fire Ant,Solenopsis invicta

Valles

2012

Psyche: A Journal of Entomology

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The imported fire ants,Solenopsis invictaandS. richteriwere introduced into the USA between 1918 and 1945. Since that time, they have expanded their USA range to include some 138 million hectares. Their introduction has had significant economic consequences with costs associated with damage and control efforts estimated at 6 billion dollars annually in the USA. The general consensus of entomologists and myrmecologists is that permanent, sustainable control of these ants in the USA will likely depend on self-sustaining biological control agents. A metagenomics approach successfully resulted in discovery of three viruses infectingS. invicta.Solenopsis invicta virus 1(SINV-1), SINV-2, and SINV-3 are all positive, single-stranded RNA viruses and represent the first viral discoveries in any ant species. Molecular characterization, host relationships, and potential development and use of SINV-1, SINV-2, and SINV-3 as biopesticides are discussed.

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“…DCV (family Dicistroviridae) is an important virus as it infects a model laboratory organism, but several other viruses in this family are also ecologically and agriculturally relevant (Bonning, 2009). Five dicistroviruses are pathogenic to beneficial invertebrate species and eight are pathogenic to pest species (Bonning and Miller, 2010). Understanding the consequences of infection for host physiology and virus pathology within this virus family would be valuable for development of antiviral protection for beneficial species and potential pest management.…”

Section: Introductionmentioning

confidence: 99%

Physiological and metabolic consequences of viral infection in Drosophila melanogaster

Arnold

Johnson

White

2013

Journal of Experimental Biology

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SUMMARYAn extensively used model system for investigating anti-pathogen defence and innate immunity involves Drosophila C virus (DCV) and Drosophila melanogaster. While there has been a significant effort to understand infection consequences at molecular and genetic levels, an understanding of fundamental higher-level physiology of this system is lacking. Here, we investigate the metabolic rate, locomotory activity, dry mass and water content of adult male flies injected with DCV, measured over the 4days prior to virus-induced mortality. DCV infection resulted in multiple pathologies, notably the depression of metabolic rate beginning 2days post-infection as a response to physiological stress. Even in this depressed metabolic state, infected flies did not decrease their activity until 1day prior to mortality, which further suggests that cellular processes and synthesis are disrupted because of viral infection. Growth rate was also reduced, indicating that energy partitioning is altered as infection progresses. Microbial infection in insects typically results in an increase in excretion; however, water appeared to be retained in DCV-infected flies. We hypothesise that this is due to a fluid intake-output imbalance due to disrupted transport signalling and a reduced rate of metabolic processing. Furthermore, infected flies had a reduced rate of respiration as a consequence of metabolic depression, which minimised water loss, and the excess mass as a result of water retention is concurrent with impaired locomotory ability. These findings contribute to developing a mechanistic understanding of how pathologies accumulate and lead to mortality in infected flies.

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Dicistroviruses

Cited by 169 publications

References 152 publications

Optimized ReplicatingRenillaLuciferase Reporter HIV-1 Utilizing Novel Internal Ribosome Entry Site Elements for Native Nef Expression and Function

Optimized ReplicatingRenillaLuciferase Reporter HIV-1 Utilizing Novel Internal Ribosome Entry Site Elements for Native Nef Expression and Function

Positive-Strand RNA Viruses Infecting the Red Imported Fire Ant,Solenopsis invicta

Physiological and metabolic consequences of viral infection in Drosophila melanogaster

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