Diclofenac biodegradation by newly isolated Klebsiella sp. KSC: Microbial intermediates and ecotoxicological assessment

Stylianou, Kyriakos C.; Hapeshi, Evroula; Vasquez, Marlen I.; Fatta‐Kassinos, Despo; Vyrides, Ioannis

doi:10.1016/j.jece.2018.04.052

Cited by 54 publications

(21 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…KSC, was recently identified and studied for DCF removal. is is the first study that points out an environmental isolated strain capable of DCF biodegradation at high concentration (70 mg•L −1 ) and in less than 72 h [35].…”

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Grandclement

Piram

Petit

et al. 2020

Journal of Chemistry

View full text Add to dashboard Cite

Since bacterial consortia involved in conventional wastewater treatment processes are not efficient in removing diclofenac (DCF), an emerging pollutant frequently detected in water bodies, the identification of microorganisms able to metabolise this pharmaceutical compound is relevant. Thus, DCF removal was investigated using bacteria isolated from aqueous stock solutions of this micropollutant and identified as Bacillus and Brevibacillus species using 16S rRNA gene sequencing. A 100% DCF removal was achieved after 17 hours of experiment at 20°C in a nutrient medium; the biodegradation kinetic followed a pseudo-first order (kbiol = 11 L·gSS−1·d−1). Quantitative assessment of DCF removal showed that its main route was biotic degradation. The main degradation product of DCF, 4′-hydroxy-diclofenac (4′-OH-DCF), was identified using liquid chromatography-electrospray ionisation high-resolution mass spectrometry. Since the ecotoxicological impact of 4′-hydroxy-diclofenac was not reported in the literature, the ecotoxicity of DCF and its metabolite were tentatively evaluated using Vibrio fischeri bioassays. Results from these tests showed that this metabolite is not more toxic than its parent compound and may hopefully be an intermediate product in the DCF transformation. Indeed, no significant difference in ecotoxicity was observed after 30 min between DCF (50 should be writtten in subscript all along the manuscript in EC50 = 23 ± 4 mg·L−1) and 4′-hydroxy-diclofenac (EC50 = 19 ± 2 mg·L−1). Besides, the study highlighted a limit of the Microtox® bioassay, which is largely used to assess ecotoxicity. The bioluminescence of Vibrio fischeri was impacted due to the production of microbial activity and the occurrence of some carbon source in the studied medium.

show abstract

Section: Resultsmentioning

confidence: 98%

“…Moreover, as far as we know, the ecotoxicity of 4′-hydroxy-diclofenac has never been evaluated on Vibrio fischeri. Recently, Stylianou et al [35] investigated the acute toxicity of DCF and the biotransformation products created by Klebssiella sp. KSC with Vibrio fischeri.…”

Section: Dcf and Its Metabolite Ecotoxicity In Simple Mediummentioning

confidence: 99%

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Grandclement

Piram

Petit

et al. 2020

Journal of Chemistry

View full text Add to dashboard Cite

show abstract

“…KDF8 [66], Labrys portucalensis F11 [67], and Klebsiella sp. KSC [68]. The ability of new isolates to degrade DCF were studied under the co-metabolic conditions with glucose, because none of them were able to metabolize DCF as a sole carbon and energy source.…”

Section: Isolation Of New Dcf-degrading Strainsmentioning

confidence: 99%

Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity

Żur

Michalska

Piński

et al. 2020

Water

View full text Add to dashboard Cite

In this study, we evaluated the impact of the successive bioaugmentation of the activated sludge (AS) with the defined bacterial consortium on the activity and functional capacity of the AS microorganisms. In parallel, the removal of low concentrations of the selected non-steroidal anti-inflammatory drugs (ibuprofen, naproxen, diclofenac) and analgesic paracetamol was studied. We found that the addition of the bacterial consortium consisting of three pharmaceuticals-degrading strains Bacillus thuringiensis B1 (2015b), Stenotrophomonas maltophilia KB2, and Pseudomonas moorei KB4 into the AS did not cause any significant changes in the biomass abundance and metabolic activity of the AS microorganisms. Although, the successive bioaugmentation of the AS caused a slight increase in the metabolic diversity, the intensity of carbohydrates usage, and metabolic richness. Microorganisms in the bioaugmented and non-bioaugmented AS were able to degrade the mixture of the analyzed drugs with similar efficiency, however, diclofenac was removed more effectively in the bioaugmented AS. Several metabolites were identified and efficiently utilized, with the exception of 4-OH diclofenac. Two new diclofenac-degrading strains assigned as Serratia proteamaculans AS4 and Rahnella bruchi AS7 were isolated from the diclofenac-treated AS.

show abstract

“…To the best of our knowledge, the endophyte Microbacterium sp. MG7 [48], a few soil bacterial strains belonging to Raoultella-Klebsiella group [49,50] and Brevibacterium sp. D4 [25] isolated from activated sludge are the only bacteria that have been found to be capable of degrading diclofenac.…”

Section: Bacterial Community Structure In the Immobilized Cell Biofilmentioning

confidence: 99%

Biodegradation Potential and Diversity of Diclofenac-degrading Microbiota in an Immobilized Cell Biofilter

et al. 2019

View full text Add to dashboard Cite

Despite that diclofenac has been embodied to the European watch list of priority substances of concern, studies on diclofenac biodegradation are limited and the diversity of diclofenac-degrading microbiota remains unknown. In this work, an immobilized cell biofilter was constructed and operated to evaluate its effectiveness to depurate high strength diclofenac wastewater and to identify the diclofenac-degrading community accommodated in activated sludge by employing high-throughput sequencing techniques. After a two-month adaptation period, biofilter removal efficiencies reached values as high as 97.63 ± 0.62%, whereas utilization of diclofenac in the immobilized cell biofilter led to a drastic pH decrease. Based on Illumina sequencing, the major bacterial taxa identified in the immobilized cell biofilter were members of the species Granulicella pectinivorans and Rhodanobacter terrae, followed by members of the species Castellaniella denitrificans, Parvibaculum lavamentivorans, Bordetella petrii, Bryocella elongata and Rhodopseudomonas palustris. The ability of such taxa to utilize a wide range of carbon sources and to effectively adapt under acidic conditions seemed to be the main parameters, which favored their prevalence in the immobilized cell biofilter. In addition, Wickerhamiella was the predominant fungal taxon in the immobilized cell biofilter, which appears to be actively involved in diclofenac degradation in activated sludge systems.

show abstract

Diclofenac biodegradation by newly isolated Klebsiella sp. KSC: Microbial intermediates and ecotoxicological assessment

Cited by 54 publications

References 29 publications

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Effects of Low Concentration of Selected Analgesics and Successive Bioaugmentation of the Activated Sludge on Its Activity and Metabolic Diversity

Biodegradation Potential and Diversity of Diclofenac-degrading Microbiota in an Immobilized Cell Biofilter

Contact Info

Product

Resources

About