Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles

Rai, Amrita; Nöthe, Heike; Tzvetkov, Nikolay; Korenbaum, Elena; Manstein, Dietmar J.

doi:10.1007/s00018-010-0590-5

Cited by 22 publications

(38 citation statements)

References 69 publications

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“…1D and E). Nevertheless, it is interesting that fatty acid addition and thus the presence of TAG stores do not significantly shorten the generation time of Dictyostelium amoebae (13,14; also data not shown). Thus, the endogenous rate of de novo fatty acid synthesis appears to be fully sufficient for normal cell division.…”

Section: Discussionmentioning

confidence: 89%

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Dictyostelium Lipid Droplets Host Novel Proteins

Barisch

Paschke

et al. 2013

Eukaryot Cell

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Across all kingdoms of life, cells store energy in a specialized organelle, the lipid droplet. In general, it consists of a hydrophobic core of triglycerides and steryl esters surrounded by only one leaflet derived from the endoplasmic reticulum membrane to which a specific set of proteins is bound. We have chosen the unicellular organism Dictyostelium discoideum to establish kinetics of lipid droplet formation and degradation and to further identify the lipid constituents and proteins of lipid droplets. Here, we show that the lipid composition is similar to what is found in mammalian lipid droplets. In addition, phospholipids preferentially consist of mainly saturated fatty acids, whereas neutral lipids are enriched in unsaturated fatty acids. Among the novel protein components are LdpA, a protein specific to Dictyostelium, and Net4, which has strong homologies to mammalian DUF829/ Tmem53/NET4 that was previously only known as a constituent of the mammalian nuclear envelope. The proteins analyzed so far appear to move from the endoplasmic reticulum to the lipid droplets, supporting the concept that lipid droplets are formed on this membrane.

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Section: Discussionmentioning

confidence: 89%

“…Experimental addition of polyunsaturated fatty acids to the medium impaired the subsequent progression of Dictyostelium through the developmental cycle (13), and monounsaturated fatty acids inhibited cellular growth (14). Addition of palmitic acid was tolerated best; it was incorporated into cellular lipids and even slightly enhanced cell growth (13).…”

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confidence: 99%

Dictyostelium Lipid Droplets Host Novel Proteins

Barisch

Paschke

et al. 2013

Eukaryot Cell

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“…73 Pigment granules (melanosomes) could be tracked by Chang et al 74 The motion of secretory granules during activation of neuroendocrine cells was characterized by Elias et al 75 Other representative studies that investigated the motion of endosomes (see Figure 3F), ribonucleoprotein granules, telomeres, puncta and other protein clusters using Diatrack include those of Rai, Serpinskaya, Ling, Kim, Trelles-Sticken, Jacobs, and Bensenor. 76–82 …”

Section: Survey Of Applicationsmentioning

confidence: 99%

Diatrack particle tracking software: Review of applications and performance evaluation

Vallotton

Oijen

Whitchurch

et al. 2017

Traffic

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Object tracking is an instrumental tool supporting studies of cellular trafficking. There are three challenges in object tracking: the identification of targets; the precise determination of their position and boundaries, and the assembly of correct trajectories. This last challenge is particularly relevant when dealing with densely populated images with low signal-to-noise ratios - conditions that are often encountered in applications such as organelle tracking, virus particle tracking, or single-molecule imaging. We have developed a set of methods that can handle a wide variety of signal complexities. They are compiled into a free software package called Diatrack. Here we review its main features and utility in a range of applications, providing a survey of the dynamic imaging field together with recommendations for effective use. The performance of our framework is shown to compare favorably to a wide selection of custom-developed algorithms, whether in terms of localization precision, processing speed, or correctness of tracks.

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“…DRP’s function in membrane remodelling events such as budding, organelle fission and fusion, endocytosis, and cytokinesis [18-21]. These proteins do not work alone; in mitochondria, multi-component machines—fission and fusion complexes—are responsible for remodelling the inner and outer mitochondrial membranes to allow a tubule to divide or fuse [5,17].…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial fission and fusion in Dictyostelium discoideum: a search for proteins involved in membrane dynamics

2012

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BackgroundMitochondrial morphology is maintained by two distinct membrane events -fission and fusion. Altering these conserved processes can disrupt mitochondrial morphology and distribution, thereby disrupting the organelle’s functionality and impeding cellular function. In higher eukaryotes, these processes are mediated by a family of dynamin-related proteins (DRP’s). In the lower eukaryotes, for instance Dictyostelium discoideum, mitochondrial fission and fusion have been implicated but not yet established. To understand the overall mechanism of these dynamics across organisms, we developed an assay to identify fission and fusion events in Dictyostelium and to assess the involvement of the mitochondrial proteins, MidA, CluA, and two DRP’s, DymA and DymB.FindingsUsing laser scanning confocal microscopy we show, for the first time, that lower eukaryotes mediate mitochondrial fission and fusion. In Dictyostelium, these processes are balanced, occurring approximately 1 event/minute. Quantification of the rates in midA-, cluA-, dymA-, or dymB- strains established that MidA appears to play an indirect role in the regulation of fission and fusion, while the DRP’s are not essential for these processes. Rates of fission and fusion were significantly reduced in cluA-cells, indicating that CluA is necessary for maintaining both fission and fusion.ConclusionsWe have successfully demonstrated that Dictyostelium mitochondria undergo the dynamic processes of fission and fusion. The classical mediators of membrane dynamics - the DRP’s – are not necessary for these dynamics, whereas CluA is necessary for both processes. This work contributes to our overall understanding of mitochondrial dynamics and ultimately will provide additional insight into mitochondrial disease.

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Dictyostelium dynamin B modulates cytoskeletal structures and membranous organelles

Cited by 22 publications

References 69 publications

Dictyostelium Lipid Droplets Host Novel Proteins

Dictyostelium Lipid Droplets Host Novel Proteins

Diatrack particle tracking software: Review of applications and performance evaluation

Mitochondrial fission and fusion in Dictyostelium discoideum: a search for proteins involved in membrane dynamics

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