Die beidseitige Dauerperfusion menschlicher Plazentenin vitro

A rapid procedure for testing the integrity of the maternal-fetal barrier in spontaneously delivered placentas

“…System [23, l, 3]. For this purpose, every ex-perimental placenta was placed in the "artificial" uterus of our experimental apparatus [20]. The fetal vessels were filled with warm normal saline solution and 2 ml aliquots of a 0.2% solution of Evans Blue were injected at 2 minute intervals into the fetal placental circulation.…”

“…On the other hand, investigators may have been impeded in their study by the uncertainty äs to whether the available placenta was injured or intact. Over several years we have developed in collaboration with GAGEL and following the experimental designs of KRANTZ and coworkers [14,15,4,8,9,13] NESBITT et al [21], äs well äs HAMRIN et al [7] a modern apparatus equipped for the bilateral long-term perfusion of spontaneously delivered human placentas [20], stressing the study of both the most favorable experimental conditions äs well äs possible interfering factors (see also KIRCHNER and LEMTIS [11,12] a lengthy perfusion experiment within a few minutes whether the maternal-fetal barrier of the experimental placenta is intact. j…”