Die Feinstruktur des Epithels des Ductus pancreaticus major des Schafes

Weyrauch, K.D.; Schnorr, B.

doi:10.1159/000144676

Cited by 16 publications

(18 citation statements)

References 5 publications

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“…In addition to brush cells and principal cells (Githens et al 1980(Githens et al , 1981(Githens et al , 1987Hubchak et al 1990), the simple epithelium of the pancreatic duct has been reported to contain different types of endocrine cells (Bendayan 1987;Lee 1988;Park and Bendayan 1992). Unlike endocrine cells, brush cells do not contain any secretory vesicles (Weyrauch and Schnorr 1976;Luciano et al 1981; our own unpublished observations) and, thus, have to be regarded as a distinct cell type that is not related to classic enteroendocrine cells of the gut. This view is further supported by the non-reactivity of brush cells with two different marker molecules of enteroendocrine cells, serotonin and chromogranin A (this study; .…”

Section: Discussionmentioning

confidence: 80%

“…Brush cells of the alimentary tract do not show any direct synaptic contacts with nerve fibers (Weyrauch and Schnorr 1976;Wattel and Greuze 1978;Trier et al 1987;Luciano and Reale 1990). This observation opened the question of how these cells may communicate with other cells.…”

Section: Possible Targets Of Brush Cellsmentioning

confidence: 99%

“…Gastrointestinal brush cells are found throughout the surface epithelium of the stomach (most numerous in a fold of the cardiac region) (Wattel and Geuze 1978), the surface and crypt epithelium of the small and large intestine (Silva 1966;Trier et al 1987), and also throughout the epithelial lining of the gall bladder, the extra-hepatic bile ducts (Luciano and Reale 1990), and the pancreatic duct (Weyrauch and Schnorr 1976;Höfer and Drenckhahn 1992). Ultrastructurally, brush cells resemble in many aspects taste receptor cells of the tongue.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the taste cell G-protein, α-gustducin, in brush cells of the rat pancreatic duct system

Höfer

Drenckhahn

1998

Histochemistry and Cell Biology

123

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The major pancreatic excretory ducts have been shown to contain a large number of specialized epithelial cells, named brush cells, that are characterized by an apical tuft of stiff microvilli. The function of pancreatic brush cells is unknown. Because of some structural similarities to taste receptor cells of the tongue, we addressed the question whether pancreatic brush cells contain the taste cell-specific GTP-binding protein, alpha-gustducin, and hence might be considered to be involved in intraductal chemoreception. By immunostaining, we show that ductal brush cells of the rat pancreatic duct system contain alpha-gustducin, which is concentrated in the apical tuft of microvilli and is also found along the basolateral cell surface. A further outcome of this study is that brush cells are concentrated in the terminal portions of extralobular ducts and in the major pancreatic duct where brush cells comprise up to 22% of the ductal epithelium. Immunoblotting of the major pancreatic duct revealed a 42-kDa band that comigrated with alpha-gustducin of the rat tongue. In view of our previous observation that the ductal brush cells are particularly rich in nitric oxide synthase-I, there is reason to assume that these cells might play a role in certain aspects of chemoreceptive signalling. Thus, chemosensory control of pancreatic secretion might occur at two independent sites, the intestine and the terminal portions of the excretory duct system.

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Section: Discussionmentioning

confidence: 80%

Section: Possible Targets Of Brush Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of the taste cell G-protein, α-gustducin, in brush cells of the rat pancreatic duct system

Höfer

Drenckhahn

1998

Histochemistry and Cell Biology

123

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“…Since then, they have been detected in a variety of mammals in lung (Meyrick & Reid, 1968), gastrointestinal (GI) tract (Luciano et al. 1968a,b), including gallbladder (where they are particularly abundant) (Luciano & Reale, 1969), pancreas (Weyrauch & Schnorr, 1976), parotid and submandibular gland (Sato & Miyoshi, 1988).…”

Section: Introductionmentioning

confidence: 99%

Brush cells in the human duodenojejunal junction: an ultrastructural study

2007

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Brush cells have been identified in the respiratory and gastrointestinal tract mucosa of many mammalian species. In humans they are found in the respiratory tract and the gastrointestinal apparatus, in both the stomach and the gallbladder. The function of brush cells is unknown, and most morphological data have been obtained in rodents. To extend our knowledge of human brush cells, we performed an ultrastructural investigation of human small intestine brush cells. Six brush cells identified in five out of more than 300 small intestine biopsies performed for gastrointestinal tract disorders were examined by transmission electron microscopy. Five brush cells were located on the surface epithelium and one in a crypt. The five surface brush cells were characterized by a narrow apical pole from which emerged microvilli that were longer and thicker than those of enterocytes. The filamentous core extended far into the cell body without forming the terminal web. Caveolae were abundant. Filaments were in the form of microfilaments and intermediate filaments. Cytoplasmic projections containing filaments were found on the basolateral surface of brush cells. In a single cell, axons containing vesicles and dense core granules were in close contact both with the basal and the lateral surface of the cell. The crypt brush cell appeared less mature. We concluded that human small intestine brush cells share a similar ultrastructural biology with those of other mammals. They are polarized and well-differentiated cells endowed with a distinctive cytoskeleton. The observation of nerve fibres closely associated with brush cells, never previously described in humans, lends support to the hypothesis of a receptor role for these cells.

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“…Tuft cells, also termed brush, fibrovesicular, multivesicular, and caveolated cells by various authors, were described in the mouse stomach and duodenum (Jarvi and Keyrilainen, 1955) and rat trachea (Rhodin and Dalhamn, 1956) over 30 years ago. These distinctive cells have now been observed in a variety of mammals in most columnar epithelia derived from endoderm including stomach (Jarvi and Keyrilainen, 1955;Isomaki, 1973;Nabeyama and Leblond, 1974;Wattel and Geuze, 1978), small and large intestine (Silva, 1966;Isomaki, 1973;Nabeyama and Leblond, 1974;Tsubouchi and Leblond, 1979), biliary tract Luciano and Reale, 1979;Luciano et al, 1981), pancreatic duct ( Weyrauch and Schnorr, 1976), and respiratory tract (Rhodin and Dalhamn, 1956;Meyrick and Reid, 1968;Allan, 1978). Moreover, their recent description in the intestine of the bullfrog tadpole (Sugimoto et al, 1983) suggests that they may well be conserved during evolution.…”

mentioning

confidence: 94%

Structural features of the apical and tubulovesicular membranes of rodent small intestinal tuft cells

et al. 1987

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Tuft cells are present in most columnar epithelia derived from endoderm including the small intestine. They are characterized by long, wide apical microvilli and an extensively developed cytoplasmic tubulovesicular system. We examined in detail the structural features of the apical plasma membrane of small intestinal tuft cells from adult guinea pigs, rats, and adult and suckling mice with freeze-fracture and conventional transmission electron microscopy methods and utilized cationized ferritin and horseradish peroxidase as tracers to determine whether tuft cells endocytose macromolecules. The microvillus membrane of intestinal tuft cells has few P-face intramembrane particles, displays little alkaline phosphatase activity, and is highly enriched in cholesterol. Tuft cell tight junctions resemble those of absorptive cells in strand count and strand-to-strand crosslinks but, unlike those of absorptive cells, they display many abluminal free-ending strands. Tuft cells of adult and suckling mouse intestine show no evidence of internalization of cationized ferritin or, in suckling mice, uptake of horseradish peroxidase. We conclude that the microvillus membrane of small intestinal tuft cells is protein-poor but cholesterol-rich and that small intestinal tuft cells do not endocytose macromolecules in bulk from the intestinal lumen.

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Die Feinstruktur des Epithels des Ductus pancreaticus major des Schafes

Cited by 16 publications

References 5 publications

Identification of the taste cell G-protein, α-gustducin, in brush cells of the rat pancreatic duct system

Identification of the taste cell G-protein, α-gustducin, in brush cells of the rat pancreatic duct system

Brush cells in the human duodenojejunal junction: an ultrastructural study

Structural features of the apical and tubulovesicular membranes of rodent small intestinal tuft cells

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