Die genetische Grundlage der Monogenie bei der Schmeißfliege Chrysomya rufifacies (Calliphoridae, Diptera)

Ullerich, Fritz-Helmut

doi:10.1007/bf00268869

Cited by 26 publications

(19 citation statements)

References 18 publications

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“…Wild-type and white mutant laboratory stocks of Chrysomya rufifaeies (Diptera, Calliphoridae) were maintained as described by Ullerich (1973). For egg collections of known sex, the white mutation (Ullerich 1973) was used as marker gene for the chromosome bearing the recessivefallele. Wild-type females were mass mated with white males.…”

Section: Methodsmentioning

confidence: 99%

“…Male and female flies have five pairs of large chromosomes and one pair of small heterochromatic chromosomes (Ullerich 1963). The genetic factor that specifies the thelygenic phenotype is localized on the proximal half of the left arm of the fifth chromosome (Ullerich 1973(Ullerich , 1975. Thelygenic females are heterozygous for the dominant or epistatic female sex realizer/7", while the arrhenogenic females and the males are homozygous for the recessive allelef It is proposed that F" controls the deposition of a substance in the egg which triggers female development.…”

Section: Introductionmentioning

confidence: 99%

“…1. Scheme of the maternal sex determination in C. rufifacies, modified afterUllerich (1973). On the left side a thelygenic female with the genotype F'/fand below, the two possible genotypes of the gamets.…”

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confidence: 99%

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The sex-lethal gene homologue in Chrysomya rufifacies is highly conserved in sequence and exon-intron organization

Müller-Holtkamp

1995

J Mol Evol

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A great variety of sex determination mechanisms exists in insect species. In Drosophila melanogaster sex is determined by the ratio between X chromosomes and autosomes, while in the blowfly Chrysomya rufifacies it is maternally determined. A cascade of genes which are involved in sex determination has been identified in D. melanogaster with the Sex-lethal gene (Sxl) as the key gene. We screened genomic libraries of C. rufifacies with a probe of the Sxl gene from D. melanogaster and isolated a genomic region that included most of the homologous gene. DNA- and protein-sequence comparison showed a high percent identity between the Chrysomya and the Drosophila gene. Up to 90% identity of the amino acid sequences was found in the region that contained the RNA-binding domains. The degree of identity is much lower outside of this functionally important region (18% identity). cDNA analysis showed a highly conserved exon-intron structure between the two species, although sex-specific splicing as used in D. melanogaster for the regulation of Sxl activity, could not be detected in C. rufifacies.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The sex-lethal gene homologue in Chrysomya rufifacies is highly conserved in sequence and exon-intron organization

Müller-Holtkamp

1995

J Mol Evol

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“…Maternal sexual predetermination does not cause sexual abnormalities in the insect and normal males or females are always produced but it does generally act on the progeny sex-ratio, by fixing the proportion of oocytes of one type or the other produced by any given female, as in Chrysomya rufifacies (Ullerich, 1975(Ullerich, , 1984, coccids (scale insects and mealybugs; Brown and Chandra, 1977) and sciarids (Metz, 1938). Although the sexual development of each insect is rigidly controlled by the cascade of sex determination genes, the predetermination mechanisms seems to be subject to less rigid genetic control mechanism (Brown and De Lotto, 1957).…”

Section: Introductionmentioning

confidence: 99%

Temperature and the progeny sex-ratio in Sciara ocellaris (Diptera, Sciaridae)

2007

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We found that the sex-ratio of an amphigenic strain of Sciara ocellaris varied widely from progenies with few males to progenies containing a larger proportion of males, with single-sex progenies being rare. The sex-ratio distributions were dependent on the temperature at which the stocks of flies were raised, with the sex-ratio distributions being symmetrical (i.e. about 50% males) at 18°C and 20°C while at the higher temperatures of 24°C and 28°C the distributions were skewed toward a high proportion of females with the mean proportion of males decreasing to about 30-37% per progeny. Temperature-shift experiments showed that high temperatures were effective only during the last stages of female pupal development plus a period after adult emergence, stages corresponding to oocyte maturation. When imagine females were exposed to temperatures as low as 12°C the sex-ratio distributions of their progeny were skewed toward a high proportion of males per progeny. No differential fecundity was involved in these progeny sex-ratio modifications. Egg-to-adult survival was lower at 18°C and 28°C but no correlations with skewing in the sex ratio distributions were observed, indicating that modifications in progeny sex-ratio did not involve the differential survival of a particular sex.

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“…The species appears to have homomorphic sex chromosomes as the genome size of males is statistically indistinguishable from females [Ullerich and Schottke, 2006;Picard et al, 2012]. Further work has demonstrated that the sexes cannot be 35 distinguished using karyotyping [Ullerich, 1963[Ullerich, , 1973[Ullerich, , 1975Ullerich and Schottke, 2006]. Based on some sexlinked translocations, the chromosome pair 5 was predicted to be the 'sex chromosomes' [Ullerich, 1975;Ullerich and Schottke, 2006].…”

Section: Sex Determination In C Rufifaciesmentioning

confidence: 99%

Sex Determination Mechanisms in the Calliphoridae (Blow Flies)

Scott

Pimsler

Tarone

2013

Sex Dev

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The Calliphoridae or blow flies are a family of insects that occupy diverse habitats and perform important ecological roles, particularly the decomposition of animal remains. Some Calliphoridae species are also important in the forensic sciences, in agriculture (e.g. as livestock pests) and in medicine (e.g. maggot therapy). Calliphoridae provide striking examples in support of the hypothesis that sex determination regulatory gene hierarchies evolve in the reverse order, with the gene at the top being the most recently added. Unlike the model fly Drosophila melanogaster, where sex is determined by the number of X chromosomes, in the Australian sheep blow fly (Lucilia cuprina) sex is determined by a Y-linked male-determining gene (M). A different regulatory system appears to operate in the hairy maggot blow fly (Chrysomya rufifacies) where the maternal genotype determines sex. It is hypothesized that females heterozygous for a dominant female-determining factor (F/f) produce only female offspring and homozygous f/f females produce only sons. The bottom of the regulatory hierarchy appears to be the same in D. melanogaster and L. cuprina, with sex-specific splicing of doublesex transcripts being controlled by the female-specific Transformer (TRA) protein. We discuss a model that has been proposed for how tra transcripts are sex-specifically spliced in calliphorids, which is very different from D. melanogaster.

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Die genetische Grundlage der Monogenie bei der Schmeißfliege Chrysomya rufifacies (Calliphoridae, Diptera)

Cited by 26 publications

References 18 publications

The sex-lethal gene homologue in Chrysomya rufifacies is highly conserved in sequence and exon-intron organization

The sex-lethal gene homologue in Chrysomya rufifacies is highly conserved in sequence and exon-intron organization

Temperature and the progeny sex-ratio in Sciara ocellaris (Diptera, Sciaridae)

Sex Determination Mechanisms in the Calliphoridae (Blow Flies)

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