It is the purpose of this report to present a simple method for testing the toxicity of chemical substances by using enchytraeids in an aquatic environment. Up to eight different environmental chemicals were applied to various species, mainly of the genus Enchytraeus. The results were compared with those achieved for D. magna. Significant differences, however, between the LC,, values of the various enchytraeid species and the LC,, values for enchytraeids and daphnids could not be observed. For E. cJ: buchholzi the toxicological sensitivity of discrete ontogenetic stages was tested. The Aquatic Enchytraeid Test results were compared with those obtained from the Terrestrial Enchytraeid Test. It was found that in soil a chemical could be 600 times less toxic than in water, although the same species (E. albidus) was used in both environments. Even more pronounced were the discrepancies between the terrestrial and aquatic toxicities when the LC,, values for earthworms and daphnids were compared.