Die Orthologie und Pathologie des Nukleinsäure- und Eiweißstoffwechsels der Zelle im Autoradiogramm

Schultze, B.

doi:10.1007/978-3-642-88276-0_3

Cited by 32 publications

(8 citation statements)

References 768 publications

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“…This has been demonstrated by electron microscopy in the bovine mammary gland where MDGI/H-FABP is heterogenously expressed in alveolar cells, apparently sparing the stem cells (Erdmann and Breter 1993). Comparison with autoradiographic data from the mouse (Schultze 1968) emphasizes that the cells expressing H-FABP frequently are mitotically inactive and have a generation time of more than 150 h (e.g. epithelia of the pre-stomach, mammary gland alveoli and uterus mucosa).…”

Section: Discussionmentioning

confidence: 86%

Histochemical localization of heart-type fatty-acid binding protein in human and murine tissues

Zschiesche

Kleine

Spitzer

et al. 1995

Histochem Cell Biol

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A b s tra c t C ellular fatty acid-binding proteins (FABP) are a highly conserved fam ily of proteins consisting o f several subtypes» am ong them the m am m ary-derived growth inhibitor (M D G I) w hich is quite hom ologous to or even identical with the heart-type FABP (H-FABP). The FABPs and M D G I have been suggested to be in volved in intracellular fatty acid m etabolism and traffick ing. Recently, evidence for grow th and differentiation regulating properties of M D G I and H -FA B P was provid ed. U sing four affinity-purified polyclonal antibodies against bovine and hum an antigen preparations, the cel lular localization of M D G I/H -FA B P in hum an and mouse tissues and organs was studied. T he antibodies were weakly cross-reactive w ith adipose tissue extracts known to lack H-FABP, but failed to react by W estern blot analysis with liver-type FABP (L-FABP) and intesti nal-type FA B P (I-FABP). M D G I/H -FA B P protein was mainly detected in m yocardium , skeletal and sm ooth m uscle fibres, lipid and/or steroid synthesising cells (ad renals, Leydig cells, sebaceous glands, lactating m am mary gland) and term inally differentiated epithelia of the respiratory, intestinal and urogenital tracts. The results provide evidence that expression o f H -FA B P is associat ed with an irreversibly postm itotic and term inally differ entiated status of cells. Since all the antisera em ployed show ed spatially identical and qualitatively equal im m unostaining, it is suggested that hum an, bovine and m ouse M D G I/H -FA B P proteins share highly hom ologous epi topes.

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Section: Discussionmentioning

confidence: 86%

Histochemical localization of heart-type fatty-acid binding protein in human and murine tissues

Zschiesche

Kleine

Spitzer

et al. 1995

Histochem Cell Biol

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“…Schultze [26]). After the autoradiographie process, single-labeled sections were stained by Nuclear Fast Red.…”

Section: Histological and Autoradiographic Preparation The Paraplastmentioning

confidence: 99%

Cytokinetics of epidermic cells in skin from human cadavers

1988

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Punch biopsies taken from the extensor side of the thigh of 35 human cadavers were incubated in vitro with radioactively labeled thymidine (3H-thymidine = 3H-TdR, 14C-thymidine = ~4C-TdR) to determine whether a relationship exists between changes in the proliferative activity of the skin and the postmortal interval after irreversible cardiac arrest. The cadavers were stored at 4°C. Cadavers with indeterminable time of death or presence of intoxication, drug therapy with cytostaic agents, or a skin disease were excluded from the study. Single, double, or multiple biopsies were performed on the same cadaver; single labeling with 3H-TdR was done in all cases; double labeling with 3H-TdR and a4C-TdR in selected cases.No relevant changes in the labeling index (mean, 2.39 _+ 1.03%) were demonstrable within the examined postmortal interval of 77.75 h. The DNA synthesis time (t~) was, on the average, 4.75 + 1.44h; a certain relationship to the postmortal interval existed since ts declined with increasing storage time. The potential doubling time (tpot) decreased accordingly, beginning with 181.7 h (mean storage time, 29.9 h) and ending with 137.7 h (mean storage time, 41.7 h). No statistically relevant differences, however, were established at the 1% level. Whereas both labeling index and tpot during the early postmortal interval are comparable with observations in live humans, ts was relatively short as compared to that for the epidermis of live humans.

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“…Weniger als insgesamt 10 Mitosen wurden beim Auszählen von über 100000 Lymphozyten unserer Patientengruppe gefunden, die etwa 2000 DNS-synthetisierende Zellen enthielten (die erwartete Mitose häufigkeit würde dagegen (Methodik [14]) etwa 200 Mitosen betra gen). Als mögliche Ursache der Diskrepanz zwischen markierten Zel len und solchen in Zellteilung könnten eine extreme Verlängerung der DNS-Synthese-Dauer, ein Liegenbleiben von Zellen in der G2-Phase oder ein Zelluntergang bzw.…”

Section: Ergebnisseunclassified