Die Reindarstellung von �thylen

Möser, Ludwig; Lindinger, Franz

doi:10.1007/bf01525423

Cited by 3 publications

(7 citation statements)

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“…Confluent primary cultures were trypsinized, seeded in a 1:3 split ratio on human fibronectin (4 pg/cm2; Collaborative Research, Inc., St. Waltham, MA), and grown to confluence in Medium 199 enriched with sodium heparin (90 pg/ml; Novo Industries, Copenhagen, Denmark), endothelial cell growth supplement (15 pg/ml; Collaborative Research, St. Waltham, MA) and 20 % human serum as described [21]. Final monolayers, in their second to fourth passage, were grown on 24-well plates (Falcon Labware, Becton Dickinson, Oxnard, CA) as described [2]. Exhibition of cytoplasmic factor VIII activity was tested by indirect immunofluorescence with rabbit anti-human von Willebrand factor antibody [21].…”

Section: Preparation Of Cultured Cellsmentioning

confidence: 99%

“…In brief, human fibroblasts were cultured for 12 days on petri dishes (Falcon) displaying multilayers of about 50 pm thickness. ECM was prepared and HUVE were seeded at an 1 : 2 split ratio as described [2].…”

Section: Preparation Of Endothelial Cell Monolayers On Extracellular mentioning

confidence: 99%

“…Engagement of neutrophils with ELAM-1 [15] and endotheliumderived IL-8 [44] has been shown to promote this change in configuration which occurs within seconds and turns into tight adherence predominated by CDllICD18. The third event is analyzed as a dynamic interplay which includes haptotaxis and finally leads to transmigration [2].…”

Section: Inhibition Of Transendothelial Migration Of Neutrophils By Cmentioning

confidence: 99%

“…Focusing on the fact that non-activated neutrophils can adhere to cytokine-activated endothelium [2] and upregulation of CD11/CD18 is not required for this interaction [24, 451, the influence of neutrophil chemotactic activation was of interest. As shown for formylated peptides, chemotactic activation caused dose-dependent inhibition of neutrophil adherence independent of the mode of endothelial activation.…”

Section: Inhibition Of Transendothelial Migration Of Neutrophils By Cmentioning

confidence: 99%

“…The ability of the vascular endothelial lining to control localization [l] and transendothelial migration [2,3] has significantly extended the understanding how the neutro-phi1 traffic is guided in early inflammation. Since induction of neutrophil adhesion was found in response to endothelial activation with IL-1 [l], TNF [4] and LPS [S], extensive work has been done to elucidate the molecular mechanism of this interaction.…”

Section: Introductionmentioning

confidence: 99%

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Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine‐activated endothelium: Correlation to the expression of L‐selectin

et al. 1993

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Non-activated neutrophils strongly adhere to cytokine-activated human umbilical vein endothelial cells (HUVE). However, activation of neutrophils by different chemotactic mediators led to potent inhibition of this endothelial-dependent interaction. For different formylated peptides, concentrations leading to maximal adherence inhibition coincided with those known for inducing maximal chemotactic migration of neutrophils. In terms of maximal adherence inhibition, a rank list was found in the order of N-formyl-Met-Leu-Phe > C5adesArg > interleukin-8 > C5a > or = leukotriene B4, whereas platelet-activating factor, and lipopolysaccharide showed no inhibition. This rank order was congruent to that of down-regulation of neutrophil L-selectin detected by the monoclonal antibody Leu-8. Moreover, the dose-dependent increase of neutrophil adherence inhibition corresponded to the loss of L-selectin expression. Concentrations higher than that required for maximal inhibition led to a dose-dependent decrease of inhibition, which was accompanied by increasing expression of neutrophil CD11/CD18. In contrast to the capacity of non-activated neutrophils to migrate across interleukin-1-activated HUVE monolayers, transmigration was significantly impaired after chemotactic activation.

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Section: Preparation Of Cultured Cellsmentioning

confidence: 99%

Section: Preparation Of Endothelial Cell Monolayers On Extracellular mentioning

confidence: 99%

Section: Inhibition Of Transendothelial Migration Of Neutrophils By Cmentioning

confidence: 99%

Section: Inhibition Of Transendothelial Migration Of Neutrophils By Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine‐activated endothelium: Correlation to the expression of L‐selectin

et al. 1993

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Dié Reibung, Wärmeleitung und Diffusion in Gasmischungen. III. Die Reibung von H₂C₂H₄‐Gemischen

Traütz¹,

Stauf²

1929

Annalen der Physik

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Vom M a x T r a u t x und Z r d e d r . Wdlh. S t a u f(16. Mitteilung aus dem Physikalisch-Chemischen Institut in Heidelberg) 1. Der Isosterie und Isobarie wegen haben wir die Reibung von N,und CO-Gemischen mit H, gemessenl), und die erwarteten Snalogien gefunden. Ein drittes Gas dieser Gruppe ist C,H,, durch Reibungsbetrag und Atomzahl von den beiden anderen unterschieden. Die Reibung liegt hier um so vie1 tiefer, daB ein Maximum bereits im MeBbereich zu erwarten war und auch gefunden wurde. Die Messungen sind ebenso angestellt wie bisher; desgl. die Berechnung der q-Werte daraus. Urn unter Zimmertemperatur messen zu konnen, wurde eine zweite Kapillare niit engerem Radius (0,135 mm) benutzt. Sie bestand aus drei nebeneinanderliegenden Teilen von je 30 cm Lange, die durch weitere U-formige Qlasrohrchen verbunden waren. Das Vorwarmerohr nmgab die Kapillare spiralformig. -41s Thermostat diente ein versilbertes LeerwandgefaB in einem mit Asbestwolle gefullten Holzkasten verpackt, als Kaltebad bei O o ein Gemisch von Wasser und fein zerstoaenem Eis, bei ungefahr-SOo eine Mischnng von fester Kohlensaure mit wasserfreiem Toluol. Um eine zwischen diesen beiden liegende Temperatur zu erhalten, wurde einige Zeit gewartet, bis das mit Kohlensaure-Toluol gefiillte GefaS sich auf etwa -40° erwarmt hatte. Durch Zugeben kleiner Stuckchen fester Kohlensaure in regelmagigen Zeitabstanden konnte die Temperatur nach einiger nbung auf ' I l , , ( ' konstant gehalten werden. Um im Kaltebad uberall gleiche Temperatur zu erhalten, ward mit 1) M.Trautz u. P. B. Baumann, Ann. d. Phys. [5] 2. S. 733. 1929.

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Die Industrie des Glykols

Schrader

1929

Angewandte Chemie

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Die Reindarstellung von �thylen

Cited by 3 publications

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Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine‐activated endothelium: Correlation to the expression of L‐selectin

Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine‐activated endothelium: Correlation to the expression of L‐selectin

Dié Reibung, Wärmeleitung und Diffusion in Gasmischungen. III. Die Reibung von H₂C₂H₄‐Gemischen

Die Industrie des Glykols

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Die Reindarstellung von �thylen

Cited by 3 publications

References 0 publications

Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine‐activated endothelium: Correlation to the expression of L‐selectin

Chemotaxins inhibit neutrophil adherence to and transmigration across cytokine‐activated endothelium: Correlation to the expression of L‐selectin

Dié Reibung, Wärmeleitung und Diffusion in Gasmischungen. III. Die Reibung von H2C2H4‐Gemischen

Die Industrie des Glykols

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Dié Reibung, Wärmeleitung und Diffusion in Gasmischungen. III. Die Reibung von H₂C₂H₄‐Gemischen