Die Zona pellucida des S�ugetiereies

Dietl, Johannes

doi:10.1007/bf00365833

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…-sera of fertile women (4,6). In addition these antiThe zona pellucida is an exträcellular glycoprotein zona pellucida-antibodies have also been found in matrix surrounding the mammalian oocyte (1). Be^ sera of fertile men (4,13).…”

Section: Introductionmentioning

confidence: 99%

Solid-Phase Antigen Immunoassay for the Detection of Anti-Zona Pellucida-Antibodies in Clinically Defined Sera

Czuppon¹,

Dietl²,

Tripatzis³

1987

Clinical Chemistry and Laboratory Medicine

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Summary:A new solid-phase antigen immunoassay was developed for the detection of anti-zona pellucidaantibodies. The assay was validated in a double blind study with clinically defmed sera. The new assay is easy to perform and large numbers of sera can be processed in one day. The antigen can be prepared in advance and stored until use. This represents an advantage over other methods, such äs immunofluorescence and RIA, which require fresh antigen preparation for each set of assays. With this new test ca. 7% of females and ca. 20% of males with unexplained infertility have shown elevated antibody concentrations compared with fertile controls. The estimated intra-assay and inter-assay CV were 8.5% and less than 10% respectively.

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Section: Introductionmentioning

confidence: 99%

Solid-Phase Antigen Immunoassay for the Detection of Anti-Zona Pellucida-Antibodies in Clinically Defined Sera

Czuppon¹,

Dietl²,

Tripatzis³

1987

Clinical Chemistry and Laboratory Medicine

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Polarized distribution of binding sites for concanavalin A and wheat-germ agglutinin in the zona pellucida of goodeid oocytes (teleostei)

Schindler

Vries

1989

Histochemistry

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Zonae pellucidae of the viviparous goodeid teleosts Girardinichthys viviparus, Xenoophorus captivus, and Xenotoca eiseni were investigated ultrastructurally, and binding sites for ConA and WGA were localized on cross-sections using a colloidal gold technique. In late stages of development, the oocytes are surrounded by a three-zonated acellular matrix multiply perforated by pore canals allowing long microvilli of the oocyte to penetrate interstices of the follicle epithelium. Together, the surface of the microvilli and zona pellucida is coated by a thin layer of homogeneous slightly electron-dense material. In early oogenesis, the thin acellular layer is entirely packed with binding sites for WGA, whereas those for ConA occur only sparsely. Three-zonated zonae pellucidae amply contain both WGA and ConA receptors. The asymmetric labelling pattern obtained with both lectin protein gold preparations indicates a polarized organization of the different glycoconjugates. WGA receptors are concentrated within the outer region of the zona pellucida. Labelling with ConA-HRP-Au complexes produced heavy deposits of marker beads within the inner two thirds of the zona pellucida and weak labelling of the superficial coat. After prolonged digestion with neuraminidase, WGA binding sites were no longer detectable.

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Die Zona pellucida des S�ugetiereies

Cited by 2 publications

References 40 publications

Solid-Phase Antigen Immunoassay for the Detection of Anti-Zona Pellucida-Antibodies in Clinically Defined Sera

Solid-Phase Antigen Immunoassay for the Detection of Anti-Zona Pellucida-Antibodies in Clinically Defined Sera

Polarized distribution of binding sites for concanavalin A and wheat-germ agglutinin in the zona pellucida of goodeid oocytes (teleostei)

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