Diel photosynthetic production of cellular organic matter in natural phytoplankton populations, measured with13C and gas chromatography/mass spectrometry

Hama, Junko; Handa, Nobuhiko

doi:10.1007/bf00702460

Cited by 8 publications

(5 citation statements)

References 38 publications

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“…Moreover, the ribose content increased during the growth of the alga. This is in agreement with earlier studies reporting the abundance of ribose and ribose containing compounds during the growth of algae and natural phytoplankton (Weiler & Karl, 1979;Hama & Handa, 1992).…”

Section: Discussionsupporting

confidence: 94%

“…and glucose. These monosaccharides are of common occurrence in the carbohydrates of marine diatoms (Handa, 1969;Hecky et al, 1973;Haug & Myklestad, 1976;Parsons, 1984b;Hama & Handa, 1992). Nevertheless, the relative contributions of the individual sugars varied.…”

Section: Discussionmentioning

confidence: 97%

“…From this it can be concluded that the synthesis of high molecular weight polymers containing predominantly glucose was responsible for the observed abundance of carbohydrate in A. coffeaeformis cells. Such polymers have been recognized widely as algal storage material (Handa, 1969;Cuhel^a/., 1984;Varum etal, 1986;Hama & Handa, 1992).…”

Section: Discussionmentioning

confidence: 99%

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Carbohydrate production byAmphora coffeaeformis, a marine fouling diatom

1993

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Amphora coffeaeformis var. perpusilla (Grunow) Cleve was grown under continuous illumination at 18°C in batch cultures using f/2 medium. Cell biomass (measured as dry weight, chlorophyll a, protein and carbon) reached a maximum on day 5. Thereafter cell biomass either remained constant or showed a small decrease. Cellular carbohydrate and protein were the major biochemical constituents. In contrast to cell protein, cellular carbohydrate showed continuous increase with time. The carbohydrate group composition data indicate that acid soluble carbohydrate was the major component of cellular carbohydrate. The observed increase in cellular carbohydrate was due to acid soluble carbohydrate which accounted for 56% of the total cell carbon. Protein:carbohydrate ratios ranged from 1·5 to 0·2 over the period of culture, corresponding to the ratios observed in cultures developing nitrogen deficiency. Monosaccharide analysis of the algal cells indicated that glucose was the most abundant monosaccharide.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 97%

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Carbohydrate production byAmphora coffeaeformis, a marine fouling diatom

1993

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“…1) and a strong correlation between glucose concentrations and chlorophyll fluorescence (Table 1) were observed in the present study. Previous studies of dissolved carbohydrates in surface ocean waters have also noted a diel pattern with higher concentrations during daylight hours (Burney et al 1982;Burney 1986;Hama and Handa 1992). Studies have shown that algae exude large amounts of carbohydrates (e.g., Mopper et al 1995;Biddanda and Benner 1997 and references therein), and herbivorous grazing releases dissolved carbohydrates (Strom et al 1997).…”

Section: Discussionmentioning

confidence: 93%

Bacterial utilization of dissolved glucose in the upper water column of the Gulf of Mexico

Skoog¹,

Biddanda

Benner

1999

Limnology & Oceanography

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Several aspects of bacterial glucose assimilation and bacterial production (BP) were investigated over 2 d in the upper 300 m of the Gulf of Mexico. Glucose concentrations ranged from 2 to 15 nM in surface seawater; glucose utilization rates were 1-3 nM d Ϫ1 in the upper 150 m of the water column and 0.02-0.8 nM d Ϫ1 in deeper water (150-300 m). Turnover of glucose and bacteria were similar and were in the range of 0.01-0.4 d Ϫ1 . Measured glucose assimilation rates imply that glucose supports ϳ5-10% of the observed BP in surface waters. The limiting factors for bacterial glucose utilization varied with depth. Glucose assimilation seemed to be limited by availability of inorganic N in the chlorophyll maximum. Additions of nitrate or ammonium had similar effects on glucose assimilation. BP was limited by C below and above the chlorophyll maximum. Additions of high-molecular-weight (HMW) dissolved organic matter (DOM) decreased bacterial glucose uptake rates, indicating that substances competing with glucose assimilation were derived from HMW DOM extracellularly. Thus, the measurement of free glucose assimilation appears to integrate bacterial utilization of combined glucose and indicates that many of the free sugars in seawater may be derived from polymers. The higher glucose assimilation rates achieved with inorganic nutrient additions indicate that bacterial degradation of C-rich organic matter (OM) could vary between ocean regions with varying inorganic nutrient concentrations.

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“…Nevertheless, GC was used for analysis of dissolved carbohydrates (SAKUGaWa & HANDA 1983;Come & HEDGES 1984;HAMA & HANDA 1992). Gaschromatography (GC) and liquid chromatography (LC) were used.…”

Section: Carbohydratesmentioning

confidence: 99%