Dielectric Characterization of the Yeast Cell Budding Cycle

Franco, Jose Luis Sebastian; Otero, Aranzazu Sanchis; Madronero, Jose Roldan; Martin, and Sagrario Munoz San

doi:10.2528/pier12100406

Cited by 18 publications

(5 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…U wb is the average potential applied to the experiment, U box is the potential applied to the simulation box in order to simulate the current density j . n.a.=not applicable, E. coli values from [48] , [49] , S. cerevisiae values from [50] , B. subtilis subtilis thickness values from [51] and values for permittivity and conductivity are approximated by values for S. aureus and S. epidermidis from [52] , [48] , respectively. Cytoplasm values for THP-1 monocytes are derived from [53] , membrane values from [54] .…”

Section: Resultsmentioning

confidence: 99%

Behavioral study of selected microorganisms in an aqueous electrohydrodynamic liquid bridge

Paulitsch-Fuchs

Zsohár²,

Wexler³

et al. 2017

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

An aqueous electrohydrodynamic (EHD) floating liquid bridge is a unique environment for studying the influence of protonic currents (mA cm−2) in strong DC electric fields (kV cm−1) on the behavior of microorganisms. It forms in between two beakers filled with water when high-voltage is applied to these beakers. We recently discovered that exposure to this bridge has a stimulating effect on Escherichia coli.. In this work we show that the survival is due to a natural Faraday cage effect of the cell wall of these microorganisms using a simple 2D model. We further confirm this hypothesis by measuring and simulating the behavior of Bacillus subtilis subtilis, Neochloris oleoabundans, Saccharomyces cerevisiae and THP-1 monocytes. Their behavior matches the predictions of the model: cells without a natural Faraday cage like algae and monocytes are mostly killed and weakened, whereas yeast and Bacillus subtilis subtilis survive. The effect of the natural Faraday cage is twofold: First, it diverts the current from passing through the cell (and thereby killing it); secondly, because it is protonic it maintains the osmotic pressure in the cell wall, thereby mitigating cytolysis which would normally occur due to the low osmotic pressure of the surrounding medium. The method presented provides the basis for selective disinfection of solutions containing different microorganisms.

show abstract

Section: Resultsmentioning

confidence: 99%

Behavioral study of selected microorganisms in an aqueous electrohydrodynamic liquid bridge

Paulitsch-Fuchs

Zsohár²,

Wexler³

et al. 2017

Biochemistry and Biophysics Reports

View full text Add to dashboard Cite

show abstract

“…Recent studies showed that the DEP characteristic of yeast cells varies widely, depending on the cell size, shape, and electric properties of cell contents and membrane. 18,31 In particular, the reported values of the crossover frequency where the value of Re(b) of the cell changes its sign (e.g., from negative to positive) have a range that spans one order of magnitude of the field frequency. This situation may be prominent for cultured yeast cells because of the budding process, which leads to a change in shape and size during growth.…”

Section: Resultsmentioning

confidence: 99%

Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution

et al. 2016

View full text Add to dashboard Cite

The variability in cell response to AC electric fields is selective enough to separate not only the cell types but also the activation states of similar cells. In this work, we use dielectrophoresis (DEP), which exploits the differences in the dielectric properties of cells, to separate nonviable and viable cells. A parallel-plate DEP device consisting of a bottom face with an array of micro-fabricated interdigitated electrodes and a top face with a plane electrode was proposed to facilitate the separation of cells by creating a nonuniform electric field throughout the flow channel. The operation and performance of the device were evaluated using live and dead yeast cells as model biological particles. Further, numerical simulations were conducted for the cell suspensions flowing in a channel with a nonuniform AC electric field, modeled on the basis of the equation of motion of particles, to characterize the separation efficiency by changing the frequency of applied AC voltage. Results demonstrated that dead cells traveling through the channel were focused onto a site around the minimum electric field gradient in the middle of the flow stream, while live cells were trapped on the bottom face. Cells were thus successfully separated under the appropriately tuned frequency of 1 MHz. Predictions showed good agreement with the observation. The proposed DEP device provides a new approach to, for instance, hematological analysis or the separation of different cancer cells for application in circulating tumor cell identification. Published by AIP Publishing.

show abstract

“…In addition, plant cells, Gram-negative bacteria like Escherichia coli ( E. coli ), and fungi like Saccharomyces cerevisiae ( S. cerevisiae ) have outer cell walls. The electrical model of these cells could be represented by “multi-shell model” [ 31 ]. Based on MMT, these electrical models could be simplified step by step until it becomes a homogeneous sphere according to following equation:

where R i ( i = 1, 2, 3, 4) and

( i = 1, 2, 3, 4) are the radius and the complex permittivity of each shell.…”

Section: Theory and Modelingmentioning

confidence: 99%

Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

et al. 2021

View full text Add to dashboard Cite

Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.

show abstract

Dielectric Characterization of the Yeast Cell Budding Cycle

Cited by 18 publications

References 51 publications

Behavioral study of selected microorganisms in an aqueous electrohydrodynamic liquid bridge

Behavioral study of selected microorganisms in an aqueous electrohydrodynamic liquid bridge

Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution

Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis

Contact Info

Product

Resources

About