Dielectric Properties of BAT Collagen in the Temperature Range of Thermal Denaturation

Abstract

The measurements of dielectric properties of collagen BAT in the solid state were made over a frequency of the electric field range of 10 Hz to 10 kHz and at temperatures from 298 to 503 K. The temperature dependence of the dielectric constant ε and the dielectric loss factor ε″ of collagen show that thermal denaturation involves two stages. The first one corresponds to the removal of loosely bound water. The second stage is related to the release of structural bound water and probably to the beginning of the thermal transition from triple helix to coil. The observed frequency dependence of the constants ε″ and ε″ may be due to Maxwell‐Wagner‐Sillars polarization and a lesser degree to dipolar polarization of water molecules. The method of time‐temperature superposition was used to calculate the energy of activation from dielectric date of collagen.

The effect of different methods of cross-linking of collagen on its dielectric properties

“…In addition, as follows from literature data [18], the chemical changes in the triple helical structure of the collagen molecule do not occur even though the water concentration is low of about 3%. The results of this paper and our earlier dielectric studies of collagen [27,45,50] confirm the appearance of the denaturation process in collagen above 200 o C reported by other authors. In fact, the weight loss of wet and dry samples in the range 200-230 o C given in Table I corresponds to the release of strongly bound water.…”

“…categories, the methods of water elimination from collagen even up to 0% water concentration and also the function of water in stabilizing the triple-helical structure of collagen. In vivo this protein occurs in the solid state but only in the restricted temperature range, and therefore, many works [19,20,[31][32][33]38,[41][42][43][44][45] have been devoted physical properties of collagen above physiological temperature. The temperature ranges corresponding to the decomposition of water and collagen have been established.…”

Collagen films from swim bladders: Preparation method and properties

“…These properties are relevant for the design of biocompatible polymer materials with potential application in coating cardiovascular prostheses, support for cell growth and in systems for controlled drug delivery [22]. These properties have been extensively investigated for collagen material from bovine and pig skin [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38].…”

Advanced craniosacral therapy a combination of Quantum theories and Einstein’s relativities

“…Water molecules have electric, conductive and polarization properties. Polarization waves are possible, and protons can "jump" along the collagen fi bers much faster than electrical signals which can be conducted by nerves [6].…”