Dielectric Properties of the Plasma Membrane of Cultured Murine Fibroblasts Treated with a Nonterpenoid Extract of Azadirachta indica Seeds

Bonincontro, A.; Ilio, V. Di; Pedata, Osvaldo; Risuleo, Gianfranco

doi:10.1007/s00232-007-9007-2

Cited by 13 publications

(10 citation statements)

References 20 publications

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“…The results discussed in this work show that HeLa tumor cells are sensitive to a lower dosage of MEX as compared to stabilized mouse fibroblasts 3T6. Also, the cell membrane appears to be the main target of the extract as shown by lipoperoxidation assays: this observation, in particular, validates and expands former results published by our laboratory [ 7 ].…”

Section: Introductionsupporting

confidence: 88%

“…One of the research lines developed in our laboratory is the study of the biological activity of different natural compounds [ 4 , 5 , 6 , 7 ]. In particular, we recently evaluated the effects of MEX in cultured mouse fibroblasts where we observed a cytotoxic effect induced by this methanolic extract obtained from the whole Neem oil.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, we recently evaluated the effects of MEX in cultured mouse fibroblasts where we observed a cytotoxic effect induced by this methanolic extract obtained from the whole Neem oil. Our results indicate that MEX might cause cell death via the activation of apoptotic pathway following significant membrane damage [ 4 , 5 , 6 , 7 ]. In this report we address two different questions, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Differential Cytotoxicity of MEX: a Component of Neem Oil Whose Action Is Exerted at the Cell Membrane Level

2008

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Neem oil is obtained from the seeds of the tree Azadirachta indica. Its chemical composition is very complex, being rich in terpenoids and limonoids, as well as volatile sulphur modified compounds. This work focused on the evaluation of a component of the whole Neem oil obtained by methanolic extraction and defined as MEX. Cytotoxicity was assessed on two different cell populations: a stabilized murine fibroblast line (3T6) and a tumor cell line (HeLa). The data presented here suggest a differential sensitivity of these two populations, the tumor line exhibiting a significantly higher sensitivity to MEX. The data strongly suggest that its toxic target is the cell membrane. In addition the results presented here imply that MEX may contain one or more agents that could find a potential use in anti-proliferative therapy.

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Section: Introductionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential Cytotoxicity of MEX: a Component of Neem Oil Whose Action Is Exerted at the Cell Membrane Level

2008

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“…The conductance G is informative of the membrane function in terms metabolism/ion-transport. Therefore, this simplified model is commonly accepted as a good tool to investigate the biological membrane conditions [6]- [12].…”

Section: Theory and Experimentsmentioning

confidence: 99%

Electrorotation: A Spectroscopic Imaging Approach to Study the Alterations of the Cytoplasmic Membrane

Bonincontro¹,

Risuleo

2015

AMI

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Electrorotation (ER) is a technique allowing the characterization of the surface properties of a variety of supra-molecular aggregates and living cultured cells as well as cellular organelles and biological materials in general. In particular, this technique allows measuring two important physical parameters of the cell membrane: specific capacitance and specific conductance. These parameters are strictly related to the structure/function relationships of the biological membrane; ER becomes thus a powerful means to investigate a number of phenomena involving the membrane integrity. These phenomena may originate from treatments with exogenous molecules and/or from pathological effects. Concerning these aspects, the study of the transfer of exogenous material (i.e. cat-anionic vesicles or liposomes) across the cell membrane assumes a high importance. This review is focused on the physical functioning principles of ER and on the quantitative analysis of the experimental measurements. This work also reports on different fields of application of ER with particular reference to data obtained in our laboratory. The investigation of the alterations of the cytoplasmic membrane function, as evidenced by this strategy, will be illustrated in detail.

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“…In our laboratory MEX showed a significant and differential cytotoxic action, with the cancer cells being more sensitive than the normal ones [ 18 ]. The main target of MEX is the plasma membrane which, after treatment with this extract, becomes more fluid without a substantial loss of its structural properties [ 19 ]. In addition, preliminary experiments performed in our laboratory suggest that MEX has also an antiviral activity (Berardi et al ., in preparation); in any case a similar activity of neem leaf extracts was reported in a model of Dengue virus [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Resveratrol exhibits a strong cytotoxic activity in cultured cells and has an antiviral action against polyomavirus: potential clinical use

Berardi

Ricci

Castelli³

et al. 2009

J Exp Clin Cancer Res

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Background: Resveratrol is a non flavonoid polyphenol compound present in many plants and fruits and, at especially high concentrations, in the grape berries of Vitis vinifera. This compound has a strong bioactivity and its cytoprotective action has been demonstrated, however at high concentrations the drug exhibits also an effective anti-proliferative action. We recently showed its ability to abolish the effects of oxidative stress in cultured cells. In this work we assayed the bioactivity of resveratrol as antiproliferative and antiviral drug in cultured fibroblasts. Studies by other Authors showed that this natural compound inhibits the proliferation of different viruses such as herpes simplex, varicella-zoster and influenza A. The results presented here show an evident toxic activity of the drug at high concentrations, on the other hand at sub-cytotoxic concentrations, resveratrol can effectively inhibit the synthesis of polyomavirus DNA. A possible interpretation is that, due to the damage caused by resveratrol to the plasma membrane, the transfer of the virus from the endoplasmic reticulum to the nucleus, may be hindered thus inhibiting the production of viral DNA.

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Dielectric Properties of the Plasma Membrane of Cultured Murine Fibroblasts Treated with a Nonterpenoid Extract of Azadirachta indica Seeds

Cited by 13 publications

References 20 publications

Differential Cytotoxicity of MEX: a Component of Neem Oil Whose Action Is Exerted at the Cell Membrane Level

Differential Cytotoxicity of MEX: a Component of Neem Oil Whose Action Is Exerted at the Cell Membrane Level

Electrorotation: A Spectroscopic Imaging Approach to Study the Alterations of the Cytoplasmic Membrane

Resveratrol exhibits a strong cytotoxic activity in cultured cells and has an antiviral action against polyomavirus: potential clinical use

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