Dielectric Properties of Yeast Cells Expressed With the Motor Protein Prestin

Miller, John H.; Nawarathna, Dharmakeerthi; Warmflash, David; Pereira, Fred A.; Brownell, William E.

doi:10.1007/s10867-005-6064-6

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Similar results were obtained with a radical different technology when Nawarathna, Miller and co-workers used both bipolar and tetrapolar analyzers. The former was depicted with two gold electrode and the current was sensed with a magnetic device [ 21 ], and the latter was a four gold electrodes arrangement, similar to Woodward's [ 22 ]. They could partially reproduce Woodward's result using the bipolar configuration [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Non-linear dielectric spectroscopy of microbiological suspensions

Treo

Felice

2009

BioMed Eng OnLine

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Background: Non-linear dielectric spectroscopy (NLDS) of microorganism was characterized by the generation of harmonics in the polarization current when a microorganism suspension was exposed to a sinusoidal electric field. The biological nonlinear response initially described was not well verified by other authors and the results were susceptible to ambiguous interpretation. In this paper NLDS was performed to yeast suspension in tripolar and tetrapolar configuration with a recently developed analyzer.

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Section: Introductionmentioning

confidence: 99%

Non-linear dielectric spectroscopy of microbiological suspensions

Treo

Felice

2009

BioMed Eng OnLine

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“…Enzymatic proteins are supposed to change their conformation during reactions and also by the modulation of the strong interfacial electric field. Protein conformational changes and associated ion translocation creates a nonlinear response resulting in the generation of harmonics. , Miller et al report the absence of the second harmonic and enhancement of third harmonic for membrane transport controlled by enzyme-catalyzed reactions . The generation of higher harmonics was recently described in a photochemical electron chain reaction involving methyl viologen as electron receptor system.…”

Section: Resultsmentioning

confidence: 99%

Stochastic Resonance in Electron Transfer Oscillations of Extended Viologen

et al. 2014

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Selective generation of only odd harmonics and the absence of even harmonics were never described for electrochemical oscillators though this phenomenon is known in special fields of physics and in biological systems. The heterogeneous electron transfer to the extended viologen with four repetitive viologen units yields electrochemical current oscillations. Small perturbation of the applied voltage by only a few millivolts sine wave results in current oscillations, which selectively enhance only the odd harmonic higher frequencies. The effect is explained in terms of the theory of stochastic resonance occurring in driven oscillating reactions. The described interplay of heterogeneous and homogeneous coupled electron transfers complies with the behavior of a nonlinear potential-symmetric system.

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“…The contrast between the dielectric constant of cell suspensions and that of the surrounding fluid has been shown to be frequency dependent, where with increasing frequency the dielectric constant of the cells decreases, approaching that of the suspending fluid [ Carstensen , 1967; Asami et al , 1980; Prodan et al , 2004]. This phenomenon is thought to occur as a result of numerous dispersions caused by the dielectric properties of membranes (MHz range), the relaxation of biopolymers and bound water (MHz to GHz range), and reorientation of water and other molecules (>1 GHz) [ Miller et al , 2005]. In the frequency range of ground‐penetrating radar (GPR) (MHz to GHz), dielectric spectroscopy studies of cell suspensions show that small increases in dielectric constant relative to the suspending fluid can be measured [ Carstensen , 1967; Asami et al , 1980].…”

Section: Introductionmentioning

confidence: 99%

Ground‐penetrating radar observations of enhanced biological activity in a sandbox reactor

Schillig¹,

Tsoflias²,

Roberts³

et al. 2010

J. Geophys. Res.

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[1] In this study, we evaluate the use of ground-penetrating radar (GPR) to investigate the effects of bacterial activity in water saturated sand. A 90-day laboratory-scale controlled experiment was conducted in a flow-through polycarbonate sandbox using groundwater from the Kansas River alluvial aquifer as inoculum. After 40 days of collecting baseline data, bacterial growth was stimulated in the sandbox by the addition of a carbon and nutrient solution on a weekly basis. Radar signal travel time and attenuation were shown to increase downgradient of the nutrient release wells relative to upgradient locations. After 60 days, the frequency of nutrient injections was increased to twice per week, after which gaseous bubbles were visually observed downgradient of the nutrient release wells. Visual observation of active gas production correlated spatially and temporally with a rapid decrease in radar signal travel time, confirming that GPR can monitor the generation of biogenic gases in this system. Analysis of the sediments indicated microbial lipid biomass increased by approximately one order of magnitude and there were no changes in the inorganic carbon content of bulk sediment mineralogy. These findings suggest that the increase in biomass and gas production may be responsible for the observed changes in radar signal travel time reported in this study. Therefore, this study provides evidence that GPR can be used to monitor biological activity in water saturated sand.

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Dielectric Properties of Yeast Cells Expressed With the Motor Protein Prestin

Cited by 7 publications

References 22 publications

Non-linear dielectric spectroscopy of microbiological suspensions

Non-linear dielectric spectroscopy of microbiological suspensions

Stochastic Resonance in Electron Transfer Oscillations of Extended Viologen

Ground‐penetrating radar observations of enhanced biological activity in a sandbox reactor

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