Dielectric response of biconcave erythrocyte membranes to D- and L-Glucose

Abstract

In this paper, we report on the influence of D- and L-glucose on the dielectric properties of native shaped (biconcave) human erythrocytes using time domain dielectric spectroscopy. The dielectric spectra of biconcave cells were analysed using a modified form of the model originally reported for spheroid particle suspensions (Asami and Yonezawa 1995 Biochim. Biophys. Acta. 1245 317–24) The observed increase in the specific membrane capacitance of the biconcave erythrocytes was correlated with an increase in the concentration of D-glucose. In contrast, no associated correlation was found to changes in the membrane capacitance with increasing concentrations of L-glucose. A similar analysis of the dielectric response of osmotically swollen erythrocytes to changes in D-glucose concentration revealed a significantly different calculated specific cell membrane capacitance at elevated (>12 mM) D-glucose concentrations. The paper outlines and discusses the possible biochemical mechanisms that could be responsible for the measured dielectric properties of the erythrocyte membrane capacitances.

Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

“…To analyze the dielectrophoretic responses of erythrocytes in suspension, two mathematical models previously utilized were single shell and double shell models for spherical particles with insulative shells and conductive cores. However, erythrocytes display a complex biconcave shape and agreement with these models and AC data is not robust [38][39][40]. More recent work with other cell systems has invoked multi-shelled ellipsoidal models [41], which are more applicable and the focus of future erythrocyte research.…”

A Microwave Metamaterial Inspired Sensor for Non-Invasive Blood Glucose Monitoring

“…physiological saline-glucose [24] and blood plasmaglucose solutions [25,26]. In [27,28], it was shown that glucose influences the dielectric properties of blood significantly, but a mathematical model was not published. To the authors' best knowledge, the only papers that have published the mathematical model of dielectric properties of bloodglucose solutions are [29,30].…”

d-glucose-induced alterations in the electrical parameters of human erythrocyte cell membrane

“…The glucose-induced dielectric response, appearing in an increase of the membrane permittivity close to 20 mM glucose concentration, has been discussed by Feldman et al [10][11][12][13][14] in the light of a conformational changes in the membrane glucose transporter GLUT1 following glucose uptake. Moreover, it has recently been proposed [24] that glucose transport by means of GLUT1 is regulated by binding of ATP to the transporter.…”

“…The influence of glucose on the dielectric and conductometric properties of erythrocyte cell membrane has been investigated extensively in the last few years, especially by Feldman et al [10][11][12][13][14]. These studies have shown that the electrical properties (mainly the membrane capacitance or, equivalently, the membrane permittivity) of the erythrocyte cell membrane are altered by the presence of Dglucose, while effects produced by L-glucose (a biologically inactive isomer of D-glucose) were practically negligible.…”

“…Second, we have extended the glucose concentration well above the normal physiological concentration (up to 50 mM), in order to explore a wider concentration range and to point out possible saturation effects. As we have stated above, we have investigated the effects induced by D-glucose, since it is well known that L-glucose does not alter appreciably the electrical parameters of the erythrocyte cell membrane, as shown by Feldman et al [10][11][12][13][14].…”