Dielectric spectroscopy study of specific glucose influence on human erythrocyte membranes

Abstract

Time domain dielectric spectroscopy has been used to study spherical erythrocytes, suspended in diluted phosphate buffered saline (PBS) buffers at varying concentrations of D-and L-glucose at 25˚C. The osmolarity for each glucose solution was adapted, equalling that of a 63% PBS (183 mOsm). The strong effect of the electrode polarization was corrected using the fractal approach in time domain. For analysis of the dielectric properties of suspensions of erythrocytes, the Maxwell-Wagner model is used for small volume fractions. Values of the permittivity and conductivity of the cell membrane were obtained from a fitting procedure according to the one-shell model. The non-monotonic and specific response of membrane electric properties on D-glucose concentrations were observed, with a dramatic decrease around 12 mM. No changes of membrane properties have been observed in the presence of increasing concentrations of L-glucose, the biologically inactive enantiomer of D-glucose. The effect is thus specific to D-glucose. The possible mechanism of specific cell reaction to D-glucose is discussed in this paper.

Impedance spectroscopy of solutions at physiological glucose concentrations

“…The most relevant phenomenon is the plasma sodium concentration lowering in the presence of hyperglycaemia [18,19]. In [2] it was claimed that these effects are entirely responsible for the impedance variation of blood and underlying tissues, since glucose variations do not directly affect the dielectric properties of the investigated medium in the MHz band, as also stressed in other studies from the same research group [20,21]. In fact, some references were provided to other studies where the effect of variations in glucose concentration was studied in water [22,23].…”

“…Some studies investigated the dielectric properties for different glucose concentrations of PBS buffers with suspended erythrocytes [20][21]. In [20] different glucose concentrations were considered ranging from zero to about 400 mg/dl, and the analysis was performed between 10 kHz to 100 MHz.…”

“…In [20] different glucose concentrations were considered ranging from zero to about 400 mg/dl, and the analysis was performed between 10 kHz to 100 MHz. Variations in the dielectric properties of the buffers were found for the different glucose concentrations.…”

Noninvasive Glucose Monitoring: Increasing Accuracy by Combination of Multi-Technology and Multi-Sensors

“…25,26 Primarily, a change in the dielectric properties is observed, 27 which, consequently, is reflected in changes of the whole tissue impedance.…”

The Effect of a Global, Subject, and Device-Specific Model on a Noninvasive Glucose Monitoring Multisensor System

“…The link between the measurable impact of glucose variations in the tissue and underlying biophysical processes and phenomena has been discussed in a series of publications of the Feldman group and others. [12][13][14][15][16][17] Considering in vivo evidence, it has been argued that additional sensors allow to independently characterize biophysical processes contributing to the main signal and allows for the compensation of disturbing effects. The contribution of disturbance to the major dielectric signal would depend on the individual skin characteristics, such as thickness of layers, hydration, and micro-vascularization.…”