Dielectrophoresis of proteins: experimental data and evolving theory

Hayes, Mark A.

doi:10.1007/s00216-020-02623-7

Cited by 31 publications

(37 citation statements)

References 64 publications

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“…In fact, the word 'substantially' can be considered as an understatement of the situation. As reviewed elsewhere, at least 22 different globular proteins have now been investigated for their DEP responses [3][4][5][6][7]. In all the analyses by the authors of the cited studies, the so-called Clausius-Mossotti (CM) function has been invoked.…”

Section: Introductionmentioning

confidence: 99%

“…In all the analyses by the authors of the cited studies, the so-called Clausius-Mossotti (CM) function has been invoked. However, the macroscopic electrostatic concepts and assumptions used in the theoretical derivation of CM arguably fail to describe the situation for nanoparticles, such as proteins, that possess a permanent dipole moment, interact with water dipoles of hydration, and possess other physico-chemical attributes at the molecular scale [6][7][8]. The fact that standard DEP theory does not provide a basis for understanding protein DEP is recognized as "a well-accepted paradigm, repeated in numerous studies" [6].…”

Section: Introductionmentioning

confidence: 99%

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Protein Dielectrophoresis: I. Status of Experiments and an Empirical Theory

2020

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The dielectrophoresis (DEP) data reported in the literature since 1994 for 22 different globular proteins is examined in detail. Apart from three cases, all of the reported protein DEP experiments employed a gradient field factor ∇ E m 2 that is much smaller (in some instances by many orders of magnitude) than the ~4 × 1021 V2/m3 required, according to current DEP theory, to overcome the dispersive forces associated with Brownian motion. This failing results from the macroscopic Clausius–Mossotti (CM) factor being restricted to the range 1.0 > CM > −0.5. Current DEP theory precludes the protein’s permanent dipole moment (rather than the induced moment) from contributing to the DEP force. Based on the magnitude of the β-dispersion exhibited by globular proteins in the frequency range 1 kHz–50 MHz, an empirically derived molecular version of CM is obtained. This factor varies greatly in magnitude from protein to protein (e.g., ~37,000 for carboxypeptidase; ~190 for phospholipase) and when incorporated into the basic expression for the DEP force brings most of the reported protein DEP above the minimum required to overcome dispersive Brownian thermal effects. We believe this empirically-derived finding validates the theories currently being advanced by Matyushov and co-workers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein Dielectrophoresis: I. Status of Experiments and an Empirical Theory

2020

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“…The present report is a pioneer study that considers nonlinear behavior of PNPs. This is still an evolving theory and, as such, more research regarding this topic is required as stated in three recent review articles on protein electrokinetics [ 30 , 60 – 61 ].…”

Section: Resultsmentioning

confidence: 99%

Electrokinetic characterization of synthetic protein nanoparticles

Quevedo¹,

Lentz²,

Peña³

et al. 2020

Beilstein J. Nanotechnol.

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The application of nanoparticle in medicine is promising for the treatment of a wide variety of diseases. However, the slow progress in the field has resulted in relatively few therapies being translated into the clinic. Anisotropic synthetic protein nanoparticles (ASPNPs) show potential as a next-generation drug-delivery technology, due to their biocompatibility, biodegradability, and functionality. Even though ASPNPs have the potential to be used in a variety of applications, such as in the treatment of glioblastoma, there is currently no high-throughput technology for the processing of these particles. Insulator-based electrokinetics employ microfluidics devices that rely on electrokinetic principles to manipulate micro- and nanoparticles. These miniaturized devices can selectively trap and enrich nanoparticles based on their material characteristics, and subsequently release them, which allows for particle sorting and processing. In this study, we use insulator-based electrokinetic (EK) microdevices to characterize ASPNPs. We found that anisotropy strongly influences electrokinetic particle behavior by comparing compositionally identical anisotropic and non-anisotropic SPNPs. Additionally, we were able to estimate the empirical electrokinetic equilibrium parameter (eE EEC) for all SPNPs. This particle-dependent parameter can allow for the design of various separation and purification processes. These results show how promising the insulator-based EK microdevices are for the analysis and purification of clinically relevant SPNPs.

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“…An electrode with tip edges (triangular electrode) requires less applied voltage as compared to flat rectangular/square electrode edges, that can generate a strong DEP force required for effective cell separation [ 31 , 32 ]. However, it creates a strong thermal gradient across the micro channel, especially around the electrode tip thereby creating strong joule-heating region.…”

Section: Introductionmentioning

confidence: 99%

Numerical Study of Joule Heating Effects on Microfluidics Device Reliability in Electrode Based Devices

Yousuff¹,

Tirth

Irshad³

et al. 2021

Materials

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In electrode-based microfluidic devices, micro channels having narrow cross sections generate undesirable temperature inside the microfluidic device causing strong thermal distribution (joule heating) that eventually leads to device damage or cell loss. In this work, we investigate the effects of joule heating due to different electrode configuration and found that, electrodes with triangular arrangements produce less heating effect even at applied potential of 30 V, without compromising the performance of the device and separation efficiency. However, certain electrode materials have low thermal gradients but erode the channel quickly thereby affecting the reliability of the device. Our simulation also predicts optimal medium conductivity (10 mS/m with 10 V) for cells to survive inside the channel until they are selectively isolated into the collection outlet. Our investigations will aid the researchers in the designing of efficient and reliable microfluidic devices to overcome joule heating inside the microchannels.

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Dielectrophoresis of proteins: experimental data and evolving theory

Cited by 31 publications

References 64 publications

Protein Dielectrophoresis: I. Status of Experiments and an Empirical Theory

Protein Dielectrophoresis: I. Status of Experiments and an Empirical Theory

Electrokinetic characterization of synthetic protein nanoparticles

Numerical Study of Joule Heating Effects on Microfluidics Device Reliability in Electrode Based Devices

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