Dielectrophoresis tweezers for single cell manipulation

Hunt, Thomas P.; Westervelt, Robert

doi:10.1007/s10544-006-8170-z

Cited by 123 publications

(81 citation statements)

References 13 publications

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“…This technique has been widely used in biological applications to characterize yeast, 2 bacteria, 3 and mammalian cells. [4][5][6][7] Electrode based DEP (eDEP) technique is normally used to generate non-uniform electric fields in the channel. 8,9 Micro-patterned electrodes in the channel generate highly localized electric fields and trapping is observed to be concentrated around the electrodes.…”

Section: Introductionmentioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D pDEP) is presented. This technology combines the benefits of electrodebased DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D pDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 lm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D pDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 ll/h and 70% at flow rates as high as 750 ll/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed at applied voltages as low as 50 Vpp. We demonstrate selective trapping of live and dead bacteria at frequencies ranging from 30 to 60 kHz at 400 Vpp with over 90% of the live bacteria trapped while most of the dead bacteria escape. V C 2015 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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“…138 The cell could be captured by the positive DEP force and released by the negative DEP force. 139,140 Similarly, the electric field cages or wells can be formed by special electrode shape and signal configuration to capture single cells. [141][142][143] However, this method needs visual feedback and complex control and would totally stop working once out of the liquid environment.…”

Section: Dielectrophoretic Trapsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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“…6,7 The dielectrophoretic force has been used to make contact less tweezers for single cell manipulation. 8 Furthermore, it has been used to control biomolecules, 9 DNA, 10,11 nanowires, 12 bundles of carbon nanotubes, 13 and single-walled carbon nanotubes. 14 Polarization forces are also used in electrohydrodynamic continuous flow micropumps 15,16 and to operate dropletbased lab-on-a-chip.…”

Section: Introductionmentioning

confidence: 99%

Modeling the Kelvin polarization force actuation of micro- and nanomechanical systems

Schmid

Hierold

Boisen

2010

Journal of Applied Physics

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Polarization forces have become of high interest in micro-and nanomechanical systems. In this paper, an analytical model for a transduction scheme based on the Kelvin polarization force is presented. A dielectric beam is actuated by placing it over the gap of two coplanar electrodes. Finite element method simulations are used to characterize the scheme and to evaluate a field correction factor, which results from simplifying the form of the electric field. The model has been shown to be valid for dielectrics with different permittivities. The presented model facilitates the design of microresonators and nanoresonators with dielectric actuation, which offers a great freedom in the choice of structural material.

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Dielectrophoresis tweezers for single cell manipulation

Cited by 123 publications

References 13 publications

Three dimensional passivated-electrode insulator-based dielectrophoresis

Three dimensional passivated-electrode insulator-based dielectrophoresis

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Modeling the Kelvin polarization force actuation of micro- and nanomechanical systems

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