Dielectrophoretic separation of Bacillus subtilis spores from environmental diesel particles

Fatoyinbo, Henry O.; Hughes, Michael; Martin, Stacey P.; Pashby, Paul; Labeed, Fatima H.

doi:10.1039/b614556f

Cited by 26 publications

(25 citation statements)

References 15 publications

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“…In 2007, Fatoyinbo et al designed a microdevice for the separation of biological agents and achieved the removal of 99% of diesel particulate matter from an airborne environmental sample [154]. In the study, B. anthracis, considered a Figure 6.…”

Section: Dep For Air Quality Assessmentmentioning

confidence: 99%

Dielectrophoretic monitoring of microorganisms in environmental applications

Jesús‐Pérez¹,

Lapizco‐Encinas²

2011

Electrophoresis

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Dielectrophoresis (DEP) is the motion of polarizable particles in the presence of nonuniform electric fields. This novel electrokinetic technique has successfully been employed in many miniaturized systems for the manipulation and detection of microbes. This review article depicts the application of dielectrophoresis for the monitoring of microorganisms in microfluidic devices for environmental applications. The research studies described here are mainly conceived for water- and air-monitoring assessments, and are classified considering the target aimed to detect, concentrate, and/or separate, including chemical and toxicant agents, and microorganisms ranging from virus to protozoa. Dielectrophoresis has also played an important role in biofilm formation studies. This review article comprises mainly studies published from 2000 to present. Even in this relatively short time frame, there have been many significant contributions of this powerful and nascent technique related to environmental monitoring; thus, unveiling its great potential for future research directions.

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Section: Dep For Air Quality Assessmentmentioning

confidence: 99%

Dielectrophoretic monitoring of microorganisms in environmental applications

Jesús‐Pérez¹,

Lapizco‐Encinas²

2011

Electrophoresis

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“…DEP has the potential to reduce time and cost for rapid detection in bioterrorism, the evaluation of disease causing agents, as well as monitoring water and food supplies for safety. Most of the recent work is focused on the separation of pathogen types from each other or other particle types [26][27][28][29], determining viability or life stage of pathogens [30][31][32][33][34][35], or pathogen separation from environmental samples [36][37][38]. Additionally, other work builds on fundamental understanding of the effects of DEP fields on pathogens [39][40][41][42][43] and single cell manipulation [44].…”

Section: Pathogens: Bacteria Spores and Virusesmentioning

confidence: 99%

Bioanalytical separations using electric field gradient techniques

2009

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The field of separations science will be strongly impacted by new electric-field-gradient-based strategies. Many new capabilities are being developed with analytical targets ranging from particles to small molecules, and soot to living cells. Here we review the emerging area of electric field gradient techniques, dividing the large variety of techniques by the target of separation. In doing so, we have contributions using dielectrophoresis, electric field gradient focusing (including dynamic, true moving bed, and pulsed field), electrocapture and electrophoretic focusing, temperature gradient focusing, and focusing with centrifugal force. We cover the literature from the start of 2007 to June 2008, along with some introductory discussions. Even with the relatively short time frame, this young and dynamic field of inquiry produced some 100 contributions describing new and unique techniques and several new applications.

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“…For example, DEP has been used to separate bacteria from water [56][57][58], erythrocytes [59][60][61], yeast [62][63][64][65][66][67], and sample debris [68][69][70][71][72][73][74]. Bacteria has also been separated from other bacteria based on differing genera [61,62,71,72,75,76], species [57], and serotypes [77].…”

Section: Raman Studies Of Mycobacteriummentioning

confidence: 99%

The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria

et al. 2016

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Traditional bacterial analyses take one to two days under favorable conditions where the bulk of the time is spent waiting for bacteria to divide and grow until visual colonies can be observed for identification. In the case of bacteria with slow doubling times, this process can take weeks. This delay in analysis is unacceptable, especially in cases of life threatening diseases or emergencies. It is clear that in order to decrease the analysis time of the bacteria, the culturing and growth step must be circumvented. The goal of this research is to design, build, and test a device that could decrease the analysis time of bacteria using label-free methods of dielectrophoresis and Raman spectroscopy.Testing for device design was performed with clinical samples in mind, which consist of bacteria grown in a variety of environmental conditions (i.e. available food sources, growth stage, temperature, etc.) and accompanied by sample debris. Raman spectra of bacteria grown in varying media and metabolic stages were collected and analyzed. Results indicate that growth phase and media have an impact on Raman spectra iv and is distinguishable by linear discriminant analysis (LDA). Despite these spectral differences, it was found that LDA classification of closely related bacteria remains fairly high (90%) regardless of growth phase. Sample debris were also considered in device design and accommodated for by dielectrophoresis. Devices were built with the goal to isolate bacteria from a mixed sample and simultaneously acquire Raman spectra for identification.For this dissertation, a device was designed, built, and tested that incorporates dielectrophoresis for particle isolation and Raman spectroscopy for identification. The device was modeled in COMSOL to ensure that an appropriate electrical field gradient could be obtained to isolate bacteria from 5 µm diameter polystyrene spheres. The device was built and successfully trapped bacteria away from polystyrene spheres and Raman spectra of the bacteria were collected while trapped. These results indicate a clear potential for contactless dielectrophoresis-Raman devices to isolate and identify bacteria from sample debris, and thereby decrease the analysis time of bacteria. Typical bacterial analysis involves culturing and visualizing colonies on an array of agar plates. The growth patterns and colors among the array are used to identify the bacteria. For fast growing bacteria such as Escherichia coli, analysis will take one to two days. However, slow growing bacteria such as mycobacteria can take weeks to identify.In addition, there are some species of bacteria that are viable but nonculturable. This lengthy analysis time is unacceptable for life-threatening infections and emergency situations. It is clear that to decrease the analysis of the bacteria, the culturing and growth steps must be avoided. The goal of this research is to design, build, and test a device that could decrease the analysis time of bacteria.

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Dielectrophoretic separation of Bacillus subtilis spores from environmental diesel particles

Cited by 26 publications

References 15 publications

Dielectrophoretic monitoring of microorganisms in environmental applications

Dielectrophoretic monitoring of microorganisms in environmental applications

Bioanalytical separations using electric field gradient techniques

The use of microfluidics and dielectrophoresis for separation, concentration, and identification of bacteria

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