Diencephalic placement of estradiol and sexual receptivity in the female rat

Lisk, Robert D.

doi:10.1152/ajplegacy.1962.203.3.493

Cited by 231 publications

(70 citation statements)

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“…The clearest differences were apparent in the ventromedial nucleus and preoptic area. Electrical or chemical stimulation of the ventromedial nucleus facilitates feminine sexual behavior (Lisk, 1962;Barfield and Chen, 1977;Pfaff and Sakuma, 1979), but does not alter masculine sexual behavior (Dorner et aI. , 1968).…”

Section: Discussionmentioning

confidence: 99%

Local Cerebral Glucose Utilization in Normal Female Rats: Variations during the Estrous Cycle and Comparison with Males

Nehlig

Porrino

Crane

et al. 1985

J Cereb Blood Flow Metab

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Summary:The quantitative 2-[14C]deoxyglucose autora diographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. A consistent though statistically nonsignificant cyclic variation in average glucose utili zation of the brain as a whole was observed. Highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. Statistically significant fluctuations were found specifically in the hypothalamus and in some limbicThe quantitative 2-[ 1 4 C]deoxyglucose autoradio graphic method (Sokoloff et aI., 1977) has been ex tensively used to determine local CMR g 1u in discrete areas of the brain in a variety of physiologic, phar macologic, and behavioral states (Sokoloff, 1981). Although there is generally equal interest in the ef fects of these experimental conditions in both sexes, males have been routinely used to avoid pos sible complications arising from the cyclicity in fe males of both reproductive and nonreproductive be haviors.In the female rat, the 4-to 5-day estrous cycle consists of four well-defined stages that are regu lated by interactions of brain, pituitary, and ovaries and by the hormones produced by each. Briefly, during metestrus, levels of estrogen and proges terone are low, as are levels of luteinizing hormone 393structures. Rates of glucose utilization in the female rat brain were compared with rates in normal male rats. Sta tistically significant differences between males and fe males at any stage of the estrous cycle were confined mainly to hypothalamic areas known to be involved in the control of sexual behavior. Glucose utilization in males and females was not significantly different in most other cerebral structures.

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Section: Discussionmentioning

confidence: 99%

Local Cerebral Glucose Utilization in Normal Female Rats: Variations during the Estrous Cycle and Comparison with Males

Nehlig

Porrino

Crane

et al. 1985

J Cereb Blood Flow Metab

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“…In the rat [L is k , 1960], rabbit [D a v id s o n and S a w y e r , 1961] and hamster [L is k and F e r g u s o n , in press], estrogen acts at the same site, namely the median eminence, to inhibit ovulation. In contrast, the estrogen sensitive sites mediating sexual receptivity are located in the pre-optic area in the rat [Lisk, 1962], the premammillary nucleus in the rabbit [P a l k a and S a w y e r , 1966], and the anterior hypothalamic area -ven tral filiform nucleus in the hamster. The significance of this variation in the functional anatomy of the hypothalamus is not known but may reflect the evolution of adaptations allowing each species to utilize different environmental and social cues to order their reproduction.…”

Section: Discussionmentioning

confidence: 99%

Central Control of Estrous Behavior in the Female Golden Hamster. Estrogen Sensitivity within the Hypothalamus

Ciaccio¹,

Lisk²

1973

Neuroendocrinology

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Following a period of estrogen priming, the spayeds hamster displays normal estrous behavior within 2–3 h after subcutaneous placement of progesterone. To determine the locus of the estrogen priming effect, a single midline implant of estradiol, fused within the lumen of a 27 or 23 gauge hypodermic tube, was stereotaxically implanted into various sites of the hypothalamus of females which had been spayed 9 days. At the time of lights out on the 3rd and on the 7th day following implantation, all females were injected with 200 µg of progesterone in 0.1 cm3 of propylene glycol. Females were tested for estrous behavior by placing them with experienced males for a period of 10 min prior to progesterone injection, and at 90 min, 2, 3, 4, 6 and 10 h thereafter. A 23 gauge estradiol implant provides a sufficient priming treatment if the hormone is placed in the anterior dorsal hypothalamus in an area including: the dorsal half of the anterior hypothalamic area, the ventral third of the filiform nucleus, and the area anterior to the dorsomedial nucleus; extending 0.5 mm laterally and 1.5 mm in the anterior-posterior plane and 1.0 mm in the dorsal-ventral plane. Implants containing radioactive estradiol indicated that the hormone did not diffuse to other areas of the hypothalamus. A 23 gauge estradiol tube was ineffective if implanted into other areas of the hypothalamus or if placed subcutaneously. A 27 gauge estradiol implant into the sensitive area did not constitute a sufficient priming treatment.

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“…Oxygen consumption [Schiaffini and M a r in , 1971; M oguilevsky and M a l in o w , 1964], C 0 2 production [Sacchi et al, 1973], succinic dehy drogenase [M oguilevsky et al, 1964], cyctochrome oxidase [M oguilevsky et al, 1971], lactic dehydrogenase [M o rishita et al, 1972] and glucose-6-phosphate dehydrogenase [H u n t er and H a g y , 1969] are elevated during the estrous phase of the cycle. The metabolic changes we have observed in the present study occur in hypothalamic nuclei which have been shown to control ovulation and sexual behavior in electrical stimulation [Teresawa and Saw y e r , 1969], lesion production [A verill and P urves, 1963;Bishop et al, 1972;C arrer et al, 1973/74 [Lisk, 1962[Lisk, ,1967 or L H -R H localization [K in g et al, 1975;Palkovitsc/ o/., 1974;W heaton et al, 1975] experiments. The exact relationship between these metabolic events and the hormone release and sexual behavior has yet to be elucidated.…”

Section: Discussionmentioning

confidence: 83%

Quantitative Histochemical Studies of the Hypothalamus. Control Point Enzymes during the Estrous Cycle

Packman¹,

Bragdon²,

Boshans³

1977

Neuroendocrinology

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Three control point enzymes of the Embden-Meyerhof pathway, hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK), have been measured in individual hypothalamic nuclei during the 5-day estrous cycle of adult rats by quantitative histochemical methods. PK levels, low during proestrus, rise to maximum activity during estrus; this rise is significantly greater than on all other days of the cycle in the lateral preoptic area (LP), ventromedial pars medialis (VMM) and pars lateralis (VML) and posterior hypothalamic (Post) nuclei. HK activity also rises from low proestrous levels during the cycle but, in contrast to PK, reaches maximum activity during diestrus-1 (D-l) or diestrus-2 (D-2). PFK showed variable changes during the estrous cycle with peaks occurring during estrus in some nuclei and during diestrus in others, but these changes were not significantly different. These metabolic changes occur in specific hypothalamic nuclei which have been shown by electrical stimulation, lesion production, stereotaxic hormone implantation and localization of luteinizing hormone-releasing factor experiments to have an important role in reproductive physiology and sexual behavior.

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Diencephalic placement of estradiol and sexual receptivity in the female rat

Cited by 231 publications

References 0 publications

Local Cerebral Glucose Utilization in Normal Female Rats: Variations during the Estrous Cycle and Comparison with Males

Local Cerebral Glucose Utilization in Normal Female Rats: Variations during the Estrous Cycle and Comparison with Males

Central Control of Estrous Behavior in the Female Golden Hamster. Estrogen Sensitivity within the Hypothalamus

Quantitative Histochemical Studies of the Hypothalamus. Control Point Enzymes during the Estrous Cycle

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