Dientamoeba fragilis prevalence coincides with gastrointestinal symptoms in children less than 11 years old in Sweden

Ögren, Jessica; Dienus, Olaf; Löfgren, Sture; Einemo, Ing-Marie; Iveroth, Peter; Matussek, Andreas

doi:10.1007/s10096-015-2442-6

Cited by 30 publications

(20 citation statements)

References 24 publications

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“…A more comprehensive study with a broad age distribution is needed to resolve this issue. These limitations aside, this finding is similar to studies showing trends of higher infection rates in adults [9,14,37] and in contrast to some reports suggesting that children are common D. fragilis carriers [9,[38][39][40]. Other studies have shown no influence of gender or age on rates of D. fragilis infection [10,27].…”

Section: Discussioncontrasting

confidence: 54%

“…Many studies have shown correlation of infection with D. fragilis and clinical symptoms [3,8,9,13,39], while others report no relationship between symptomatic infection and this parasite [44][45][46]. This disparity is not surprising, as manifestations ranging from subclinical to severe gastrointestinal symptoms is typically observed in parasitic enteropathogen infections.…”

Section: Discussionmentioning

confidence: 72%

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Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Hamidi

Meamar

Akhlaghi

et al. 2018

J Infect Dev Ctries

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Introduction: Dientamoeba fragilis, an intestinal trichomonad, occurs in humans with and without gastrointestinal symptoms. Its presence was investigated in individuals referred to Milad Hospital, Tehran. Methodology: In a cross-sectional study, three time-separated fecal samples were collected from 200 participants from March through June 2011. Specimens were examined using traditional techniques for detecting D. fragilis and other gastrointestinal parasites: direct smear, culture, formalin-ether concentration, and iron-hematoxylin staining. The presence of D. fragilis was determined using PCR assays targeting 5.8S rRNA or small subunit ribosomal RNA. Results: Dientamoeba fragilis, Blastocystis sp., Giardia lamblia, Entamoeba coli, and Iodamoeba butschlii were detected by one or more traditional and molecular methods, with an overall prevalence of 56.5%. Dientamoeba was not detected by direct smear or formalin-ether concentration but was identified in 1% and 5% of cases by culture and iron-hematoxylin staining, respectively. PCR amplification of SSU rRNA and 5.8S rRNA genes diagnosed D. fragilis in 6% and 13.5%, respectively. Prevalence of D. fragilis was unrelated to participant gender, age, or gastrointestinal symptoms. Conclusions: This is the first report of molecular assays to screen for D. fragilis in Iran. The frequent finding of D. fragilis via fecal analysis indicated the need to include this parasite in routine stool examination in diagnostic laboratories. As the length of amplification target correlates to the sensitivity of PCR, this assay targeting the D. fragilis 5.8S rRNA gene seems optimal for parasite detection and is recommended in combination with conventional microscopy for diagnosing intestinal parasites.

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Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 72%

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Hamidi

Meamar

Akhlaghi

et al. 2018

J Infect Dev Ctries

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“…Dientamoeba was associated with diarrhea, fever, vomiting, and abdominal pain in this patient group (99). Ogren et al (124) found an association between D. fragilis and GI symptoms in children aged 1 to 10 years from the county of Jönköping, Sweden.…”

Section: Clinical Aspects and Epidemiologymentioning

confidence: 99%

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

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SUMMARYDientamoeba fragilisis a protozoan parasite of the human bowel, commonly reported throughout the world in association with gastrointestinal symptoms. Despite its initial discovery over 100 years ago, arguably, we know less about this peculiar organism than any other pathogenic or potentially pathogenic protozoan that infects humans. The details of its life cycle and mode of transmission are not completely known, and its potential as a human pathogen is debated within the scientific community. Recently, several major advances have been made with respect to this organism's life cycle and molecular biology. While many questions remain unanswered, these and other recent advances have given rise to some intriguing new leads, which will pave the way for future research. This review encompasses a large body of knowledge generated on various aspects ofD. fragilisover the last century, together with an update on the most recent developments. This includes an update on the latest diagnostic techniques and treatments, the clinical aspects of dientamoebiasis, the development of an animal model, the description of aD. fragiliscyst stage, and the sequencing of the firstD. fragilistranscriptome.

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“…Organisms seen in direct wet mounts may appear as refractile, round forms; the nuclear structure cannot be seen without examination of the permanent stained smear. It has also been confirmed that molecular methods are far more sensitive than wet mounts (36). With the recent confirmation of the cyst stage, one needs to take into account the more shrunken appearance of this form compared with the trophozoite.…”

Section: Diagnosismentioning

confidence: 99%

Dientamoeba fragilis, One of the Neglected Intestinal Protozoa

Garcia¹

2016

J Clin Microbiol

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Dientamoeba fragilis is a single-celled protozoan, closely related to the trichomonads. Reported worldwide as causing human gastrointestinal symptoms, D. fragilis is very common and is second only to Blastocystis spp. Dientamoebiasis equals or exceeds the incidence of giardiasis. This minireview includes diagnostic options, clinical relevance, therapy, an animal model, the confirmed cyst stage, and sequencing data. The development of a rodent model, fulfilling Koch's postulates, and the confirmation of a cyst stage have clarified transmission routes, including fecal-oral transmission. The prevalence of D. fragilis varies between 0% to over 82%; results depend on the geographic location, group studied, and diagnostic methods used.

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Dientamoeba fragilis prevalence coincides with gastrointestinal symptoms in children less than 11 years old in Sweden

Cited by 30 publications

References 24 publications

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Dientamoeba fragilis, One of the Neglected Intestinal Protozoa

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