Diet and gender influence survival of transgenic Berkley sickle cell mice

Jahagirdar, Om B.; Mittal, Aditya; Song-Naba, Waogwende Leonce; Jha, Ritu; Kiven, Stacy B; Thompson, Susan T.; Connett, John E.; Gupta, Kalpna

doi:10.3324/haematol.2018.208322

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…The sickle diet contained increased arginine, ω-3 fatty acids and glutamate, compared to the regular diet. The observations on the effect of sickle diet on reducing pain are complemented by our recent observations on the improvement in survival of pups upto 5 months of age of sickle mice fed sickle diet compared to regular diet 117 . In this study, feeding sickle diet to the parents significantly improved the survival of male offsprings, suggesting the vulnerability of male survival in utero and post-natally and sensitivity to the environmental changes.…”

Section: Discussionmentioning

confidence: 91%

Diet and companionship modulate pain via a serotonergic mechanism

Tran

Sagi

Jarrett

et al. 2021

Sci Rep

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Treatment of severe chronic and acute pain in sickle cell disease (SCD) remains challenging due to the interdependence of pain and psychosocial modulation. We examined whether modulation of the descending pain pathway through an enriched diet and companionship could alleviate pain in transgenic sickle mice. Mechanical and thermal hyperalgesia were reduced significantly with enriched diet and/or companionship. Upon withdrawal of both conditions, analgesic effects observed prior to withdrawal were diminished. Serotonin (5-hydroxytryptamine, 5-HT) was found to be increased in the spinal cords of mice provided both treatments. Additionally, 5-HT production improved at the rostral ventromedial medulla and 5-HT accumulated at the dorsal horn of the spinal cord of sickle mice, suggesting the involvement of the descending pain pathway in the analgesic response. Modulation of 5-HT and its effect on hyperalgesia was also investigated through pharmaceutical approaches. Duloxetine, a serotonin-norepinephrine reuptake inhibitor, showed a similar anti-nociceptive effect as the combination of diet and companionship. Depletion of 5-HT through p-chlorophenylalanine attenuated the anti-hyperalgesic effect of enriched diet and companionship. More significantly, improved diet and companionship enhanced the efficacy of a sub-optimal dose of morphine for analgesia in sickle mice. These findings offer the potential to reduce opioid use without pharmacological interventions to develop effective pain management strategies.

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Section: Discussionmentioning

confidence: 91%

Diet and companionship modulate pain via a serotonergic mechanism

Tran

Sagi

Jarrett

et al. 2021

Sci Rep

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“…13,15,18,19 Obtaining large cohorts of 5-to 6-month-old male HbSS-BERK sickle mice in numbers required for a similar study was challenging because fewer male pups are born; they also have higher mortality in the early postnatal months compared with females. 21,22 HbSS-BERK feature a severe disease pathology that resembles human sickle cell anemia, involving hemolysis, reticulocytosis, anemia, extensive organ damage, reduced life span, and pain. 13,20 Mice were bred and phenotyped following established protocols as described.…”

Section: Methods Animalsmentioning

confidence: 99%

Effect of chronic opioid therapy on pain and survival in a humanized mouse model of sickle cell disease

et al. 2019

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“…All protocols using live animals must first be approved by an Institutional Animal Care and Use Committee (IACUC) and must conform to governmental regulations regarding the care and use of laboratory animals. factor in the survival of pups (Jahagirdar et al, 2017). Maintaining the sickle breeder pairs and sickle offspring up to 4-5 weeks of age on a protein-and fat-enriched sickle diet before switching to standard rodent diet increases survival.…”

Section: Notementioning

confidence: 99%

“…The breeder diet recommended below does not contain soy products and is designed to support gestation, lactation, and growth of rodents. Diet changes do not affect the health of mice instantly, but cause changes in pain and survival over time (Jahagirdar et al, 2017;Tran et al, personal comm. ).…”

Section: Notementioning

confidence: 99%

Mouse Models of Pain in Sickle Cell Disease

et al. 2018

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Sickle cell disease (SCD) is a genetic blood disorder that impacts millions of individuals worldwide. SCD is characterized by debilitating pain that can begin during infancy and may continue to increase throughout life. This pain can be both acute and chronic. A characteristic feature specific to acute pain in SCD occurs during vaso‐occlusive crisis (VOC) due to the blockade of capillaries with sickle red blood cells. The acute pain of VOC is intense, unpredictable, and requires hospitalization. Chronic pain occurs in a significant population with SCD. Treatment options for sickle pain are limited and primarily involve the use of opioids. However, long‐term opioid use is associated with numerous side effects. Thus, pain management in SCD remains a major challenge. Humanized transgenic mice expressing exclusively human sickle hemoglobin show features of pain and pathobiology similar to that in patients with SCD. Therefore, these mice offer the potential for investigating the mechanisms of pain in SCD and allow for development of novel targeted analgesic therapies. © 2018 by John Wiley & Sons, Inc.

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Diet and gender influence survival of transgenic Berkley sickle cell mice

Cited by 8 publications

References 25 publications

Diet and companionship modulate pain via a serotonergic mechanism

Diet and companionship modulate pain via a serotonergic mechanism

Effect of chronic opioid therapy on pain and survival in a humanized mouse model of sickle cell disease

Mouse Models of Pain in Sickle Cell Disease

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