Diet-induced glucose homeostasis dysregulation is enhanced by taurine supplementation in ovariectomized mice

Abstract

Low levels of estrogens are associated with obesity-related comorbidities. Mice with lower levels of estrogens are thereby more sensitive to the effects of a high-fat-diet (HFD) for the development of glucose intolerance and insulin resistance. Studies in vivo have demonstrated that taurine (TAU) supplementation prevents glucose and insulin resistance. Thus, we aimed to investigate the potential beneficial effects of TAU supplementation on glucose homeostasis of mice with low levels of estrogens fed with a HFD. 3-month-old female C57BL/6J mice underwent bilateral ovariectomy (OVX). After 1 week of recovery, mice were divided into 4 groups and either received: a standard chow diet (OVXC), chow diet plus drinking water enriched with 3% of TAU (OVXCT), HFD (OVXH), and HFD plus supplementation of TAU (OVXHT) for 14 weeks. Exposure to the HFD increased adiposity and plasma levels of glucose and insulin. Contrary to our prediction, the addition of TAU enhanced the deleterious effects of the HFD. Glucose and insulin tolerance tests (ipGTT and ipITT) indicated that mice maintained on the HFD + TAU had worse glucose intolerance and insulin resistance that was linked to lower insulin signaling in skeletal muscle and liver. Insulin secretion of isolated pancreatic islets of OVXH mice was higher than OVXC, and the addition of TAU associated with a HFD did not modulate insulin secretion, suggesting a failure of pancreatic β cells of OVXHT mice. These results suggest that despite the beneficial reports of TAU, it should be used cautiously in situations where the levels of estrogens are low.

Ação anti-diabetogênica do butirato de sódio e seu efeito protetor sobre a barreira epitelial intestinal em camundongos alimentados com dieta hiperlipídica

“…For both procedures, mice from the different experimental groups were housed individually under a light and dark cycle of 12 hours at 24ºC, with free access to water and food (Branco et al, 2019). The computerized microtomography technique was used to evaluate the fat and lean masses per total body mass (Judex et al, 2010;Raposo et al, 2015;Santos et al, 2018). Anesthetized mice (with 80 mg/kg ketamine hydrochloride and 10 mg/kg xylazine hydrochloride, intraperitoneal injection) were placed in the micro-CT scanner (Bruker -Skyscan 1178 -USA).…”