Diet Influences the Accumulation of Short-Chain Fatty Acids Associated With the Gut Microbiota in the Grass Carp (Ctenopharyngodon Idellus) Hindgut

Abstract

Diet changes the composition and function of the gut microbiota (GM). Short-chain fatty acids (SCFAs) are mainly produced through carbohydrate fermentation by the GM. It remains unclear whether changing the diet influences the accumulation of SCFAs in the grass carp (Ctenopharyngodon idellus) gut. To address this question, we compared SCFA accumulation with GM changes in the hindgut contents of grass carp in groups fed hybrid giant napier (Pennisetum sinese Roxb, HG), formula feed (FG), and broad bean (Vicia faba, BG). Our results showed that diet significantly influenced the SCFA concentrations in the hindgut of grass carp. By eliminating the influences of dietary SCFA, acetate and total SCFA accumulation in the hindgut of grass carp turned out to be positively associated with dietary cellulose contents. Most of the enriched bacterial biomarkers in the HG group that consumed the most dietary cellulose were SCFA-producing bacteria. These results implied that diet could regulate the relative abundances of potential SCFA-producing bacteria and SCFA accumulation in the grass carp hindgut. These findings shed light onto GM functions related to SCFA production and associative functions between SCFAs and the GM of grass carp.

Different Types of Non-Starch Polysaccharides Alter the Growth, Intestinal Flora and Serum Metabolite Profile of Grass Carp, Ctenopharyngodon idella

“…The enhancement of the intestinal antioxidant system by dietary NSPs may be related to their fermentation products. To our knowledge, dietary NSPs can be fermented by intestinal flora to produce large amounts of short-chain fatty acids (SCFAs) [ 4 , 16 , 50 ]. Furthermore, recent studies showed that dietary supplementation with SCFAs activated the zebrafish intestinal antioxidant defence system, including an increase in the activity of SOD, CAT and glutathione peroxidase [ 51 ].…”

Strengthening and microbial regulation mechanism of Bacillus on purification device for grass carp culture wastewater

“…Currently, the main method of aquaculture in China is intensive aquaculture, which has the advantage of increasing the output of aquatic products and profits (Edwards, 2015). However, owing to the high stocking density of aquatic animal and large amount of feed input, the discharge of aquaculture wastewater (AW) causes eutrophication, hypoxia, and other adverse effects on the natural water environment (Li et al, 2019). Therefore, efficient treatment OPEN ACCESS EDITED BY of pollutants in AW is necessary to ensure the sustainable development of the aquaculture industry.…”