Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (<i>Danio rerio</i>) with genomic hypermethylation changes in brain

Gorrochategui, Eva; Li, Junyi; Fullwood, Nigel J.; Ying, Guang‐Guo; Tian, Meiping; Li, Cui; Shen, Heqing; Lacorte, Sı́lvia; Tauler, Romà; Martin, Francis

doi:10.1093/mutage/gew050

Cited by 25 publications

(15 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The toxicity of C 60 has been described in bacteria (Freitas Cordiero et al, 2014), crustaceans (Klaper et al, 2009), bivalves (Canesi et al, 2010; Al-Subiai et al, 2012), chironomids (Waissi et al., 2017) and fishes (Ferreira et al, 2012;Gorrochategui et al, 2017) as well as the potential for trophic transfer (Fortner et al, 2010; Chen et al, 2014). Besides its inherent toxicity, C 60 has also exceptional sorption capacity towards hydrophobic chemicals (Hu et al, 2014;Velzeboer et al, 2014) that may significantly affect their bioavailability, bioconcentration and toxicity.…”

Section: Introductionmentioning

confidence: 99%

The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos

Torre

Maggioni

Ghilardi

et al. 2018

Environmental Pollution

View full text Add to dashboard Cite

This study aimed to assess the toxicological consequences related to the interaction of fullerene nanoparticles (C) and Benzo(α)pyrene (B(α)P) on zebrafish embryos, which were exposed to C and B(α)P alone and to C doped with B(α)P. The uptake of pollutants into their tissues and intra-cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C interaction with B(α)P. The carrier role of C for B(α)P was observed, however adsorption on C did not affect the accumulation and localization of B(α)P in the embryos. Instead, C doped with B(α)P resulted more prone to sedimentation and less bioavailable for the embryos compared to C alone. As for toxicity, our results suggested that C alone elicited oxidative stress in embryos and a down-regulation of proteins involved in energetic metabolism. The C + B(α)P induced cellular response mechanisms similar to B(α)P alone, but generating greater cellular damages in the exposed embryos.

show abstract

Section: Introductionmentioning

confidence: 99%

The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos

Torre

Maggioni

Ghilardi

et al. 2018

Environmental Pollution

View full text Add to dashboard Cite

show abstract

“…Results of the application of the ROIMCR procedure to other datasets from recent studies [16–22, 26–28] are briefly described in “Applications of the ROIMCR procedure” section.…”

Section: Datasetsmentioning

confidence: 99%

“…Finally, as an additional confirmation of the advantages of the ROIMCR procedure, the results obtained in the analysis of a new dataset are provided here to complete the assessment of this method. This dataset was obtained in a previous study [28] where three tissues (brain, gonads and gastrointestinal tract) were obtained from male and female zebrafish exposed to low dietary doses of four different carbon nanotubes (CBNs): C60 fullerene (C60), single-walled carbon nanotubes (SWCNT), short multi-walled carbon nanotubes (MWCNTs), and long multi-walled carbon nanotubes (MWCNTs). The lipid extracts of these samples were analyzed using LC-MS.…”

Section: Applications Of the Roimcr Proceduresmentioning

confidence: 99%

“…An example is brain tissues from females presented in Additional file 7: Figure S6, which showed very similar LC-MS elution profiles for the resolved MCR-ALS components in controls and zebrafish treatment with the distinct carbon nanoparticles. More details and specifically a discussion of the results obtained in the study of this system are provided in a published study [28] and at 10.1093/mutage/gew050. Again, the valuable contribution of the MCR-ALS methodology to the evaluation of LC-MS omic profiles in target organisms exposed to environmental contaminants has been validated.…”

Section: Applications Of the Roimcr Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

ROIMCR: a powerful analysis strategy for LC-MS metabolomic datasets

2019

Self Cite

View full text Add to dashboard Cite

Background The analysis of LC-MS metabolomic datasets appears to be a challenging task in a wide range of disciplines since it demands the highly extensive processing of a vast amount of data. Different LC-MS data analysis packages have been developed in the last few years to facilitate this analysis. However, most of these strategies involve chromatographic alignment and peak shaping and often associate each “feature” (i.e., chromatographic peak) with a unique m/z measurement. Thus, the development of an alternative data analysis strategy that is applicable to most types of MS datasets and properly addresses these issues is still a challenge in the metabolomics field. Results Here, we present an alternative approach called ROIMCR to: i) filter and compress massive LC-MS datasets while transforming their original structure into a data matrix of features without losing relevant information through the search of regions of interest (ROIs) in the m/z domain and ii) resolve compressed data to identify their contributing pure components without previous alignment or peak shaping by applying a Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) analysis. In this study, the basics of the ROIMCR method are presented in detail and a detailed description of its implementation is also provided. Data were analyzed using the MATLAB (The MathWorks, Inc., www.mathworks.com ) programming and computing environment. The application of the ROIMCR methodology is described in detail, with an example of LC-MS data generated in a lipidomic study and with other examples of recent applications. Conclusions The methodology presented here combines the benefits of data filtering and compression based on the searching of ROI features, without the loss of spectral accuracy. The method has the benefits of the application of the powerful MCR-ALS data resolution method without the necessity of performing chromatographic peak alignment or modelling. The presented method is a powerful alternative to other existing data analysis approaches that do not use the MCR-ALS method to resolve LC-MS data. The ROIMCR method also represents an improved strategy compared to the direct applications of the MCR-ALS method that use less-powerful data compression strategies such as binning and windowing. Overall, the strategy presented here confirms the usefulness of the ROIMCR chemometrics method for analyzing LC-MS untargeted metabolomics data. Electronic supplementary material The online version of this article (10.1186/s12859-019-2848-8) contains supplementary material, which is available to authorized users.

show abstract

“…Occupational exposure to specific industrial processes, such as the production and use of nanotubes or fullerenes, can induce epigenetic alterations directly or indirectly through reactive oxygen species (ROS) [ 67 – 69 ]. Occupational asthma [ 70 ] and some metabolic diseases can modify the epigenetic status and can contribute to modification of the epigenome.…”

Section: Wg 5 Hazards Characterization Risk Identification: Carcinogmentioning

confidence: 99%

Diagnosis, monitoring and prevention of exposure-related non-communicable diseases in the living and working environment: DiMoPEx-project is designed to determine the impacts of environmental exposure on human health

Budnik

Ádám

Albin

et al. 2018

J Occup Med Toxicol

View full text Add to dashboard Cite

The WHO has ranked environmental hazardous exposures in the living and working environment among the top risk factors for chronic disease mortality. Worldwide, about 40 million people die each year from noncommunicable diseases (NCDs) including cancer, diabetes, and chronic cardiovascular, neurological and lung diseases. The exposure to ambient pollution in the living and working environment is exacerbated by individual susceptibilities and lifestyle-driven factors to produce complex and complicated NCD etiologies.Research addressing the links between environmental exposure and disease prevalence is key for prevention of the pandemic increase in NCD morbidity and mortality. However, the long latency, the chronic course of some diseases and the necessity to address cumulative exposures over very long periods does mean that it is often difficult to identify causal environmental exposures.EU-funded COST Action DiMoPEx is developing new concepts for a better understanding of health-environment (including gene-environment) interactions in the etiology of NCDs. The overarching idea is to teach and train scientists and physicians to learn how to include efficient and valid exposure assessments in their research and in their clinical practice in current and future cooperative projects.DiMoPEx partners have identified some of the emerging research needs, which include the lack of evidence-based exposure data and the need for human-equivalent animal models mirroring human lifespan and low-dose cumulative exposures. Utilizing an interdisciplinary approach incorporating seven working groups, DiMoPEx will focus on aspects of air pollution with particulate matter including dust and fibers and on exposure to low doses of solvents and sensitizing agents. Biomarkers of early exposure and their associated effects as indicators of disease-derived information will be tested and standardized within individual projects. Risks arising from some NCDs, like pneumoconioses, cancers and allergies, are predictable and preventable. Consequently, preventative action could lead to decreasing disease morbidity and mortality for many of the NCDs that are of major public concern. DiMoPEx plans to catalyze and stimulate interaction of scientists with policy-makers in attacking these exposure-related diseases.Electronic supplementary materialThe online version of this article (10.1186/s12995-018-0186-9) contains supplementary material, which is available to authorized users.

show abstract

Diet-sourced carbon-based nanoparticles induce lipid alterations in tissues of zebrafish (Danio rerio) with genomic hypermethylation changes in brain

Cited by 25 publications

References 47 publications

The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos

The interactions of fullerene C60 and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish embryos

ROIMCR: a powerful analysis strategy for LC-MS metabolomic datasets

Diagnosis, monitoring and prevention of exposure-related non-communicable diseases in the living and working environment: DiMoPEx-project is designed to determine the impacts of environmental exposure on human health

Contact Info

Product

Resources

About