Dietary açai modulates ROS production by neutrophils and gene expression of liver antioxidant enzymes in rats

Guerra, Joyce Ferreira da Costa; Magalhães, Cíntia Lopes de Brito; Costa, Daniela Caldeira; Silva, Marcelo Eustáquio; Pedrosa, Maria Lúcia

doi:10.3164/jcbn.11-02

Cited by 66 publications

(43 citation statements)

References 52 publications

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“…All analyses were performed in triplicate. It is important to note that all primers used were previously tested in other studies from our lab (19,20).…”

Section: Serum Laboratory Testsmentioning

confidence: 99%

Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers

Abreu

Guerra

Pereira

et al. 2014

Arq Bras Endocrinol Metab

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Objective: This study aimed to determine whether a hypercholesterolemic diet induces hepatic steatosis, alterations in mRNA expression of NADPH oxidase subunits, and antioxidant defenses. Materials and methods: Fischer rats were divided into two groups of eight animals according to the treatment, control (C) and hypercholesterolemic diet (H). Those in group C were fed a standard diet (AIN-93M), and those of the group H were fed a hypercholesterolemic diet (25% soybean oil and 1% cholesterol). Results: The hypercholesterolemic diet did not affect body weight, but resulted in the accumulation of lipids in the liver, increased serum activities of aminotransferases and cholesterol levels. Biomarker of lipid peroxidation (TBARS) and mRNA expression of NADPH oxidase subunits p22 phox and p47 phox were increased in the liver of animals in group H. Besides, the activity and expression of antioxidant enzymes were altered. Conclusion:The results show increased mRNA expression of NADPH oxidase subunits and changes in antioxidant enzyme activities in diet-induced hepatic steatosis. Arq Bras Endocrinol Metab. 2014;58(3):251-9 Keywords Antioxidant enzymes; hepatic steatosis; hypercholesterolemic diet; NADPH oxidase; non-alcoholic fatty liver disease; oxidative stress RESUMO Objetivo: Determinar se uma dieta hipercolesterolemiante induz esteatose hepática, alterações na expressão de mRNA da NADPH oxidase e nas defesas antioxidantes. Materiais e métodos: Ratas Fischer foram divididas em dois grupos de oito animais de acordo com o tratamento recebido, controle (C) e hipercolesterolêmico (H). Aquelas do grupo C foram alimentadas com dieta padrão (AIN-93M) e as do grupo H foram alimentadas com dieta hipercolesterolemiante (25% de óleo de soja e 1% de colesterol). As dietas foram oferecidas por oito semanas. Resultados: O grupo H apresentou acúmulo de lipídios no fígado, aumento das atividades de ALT e AST e da concentração de colesterol no soro comparado ao grupo C. O marcador da peroxidação lipídica (TBARS) e os níveis de mRNA das subunidades p47 phox da NADPH-oxidase e p22 phox foram aumentados no fígado de animais do grupo H, além de alteração da atividade e expressão de enzimas antioxidantes. Conclusão: Os resultados mostram um aumento na expressão de subunidades da NADPH oxidase e alterações na atividade das enzimas antioxidantes na esteatose hepática induzida por dieta hipercolesterolemiante. Arq Bras Endocrinol Metab. 2014;58(3):251-9 Descritores Enzimas antioxidantes; esteatose hepática; dieta hipercolesterolemiante; NADPH oxidase; doença hepática gordurosa não alcoólica; estresse oxidativo

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“…All analyses were performed in triplicate. It is important to note that all primers used were previously tested in other studies from our lab (19,20).…”

Section: Serum Laboratory Testsmentioning

confidence: 99%

Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers

Abreu

Guerra

Pereira

et al. 2014

Arq Bras Endocrinol Metab

Self Cite

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“…In recent years, Aç ai has gained attention because of its potent antioxidant properties. Biochemical studies have demostrated that Aç ai is rich in phytochemicals, particularly polyphenols such as anthocyanins, proanthocyanidins and other flavonoids (Guerra et al, 2011;Xie et al, 2011) Vitamin C (ascorbic acid) is an important nutrient obtained from food and, is needed for normal metabolic processes in the body such as the formation of bone, teeth and collagen as well as wound healing (Naziroğlu et al, 2011). Many studies have reported that vitamin C may be useful for treating chronic diseases such as cancer and cataract (Park;2013;Ravindran et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Açai in Combination with Vitamin C against Aluminum-Induced Toxicity in Rat Liver

Ahmed

Hamaad²

2017

JBLS

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Aluminum is associated with the pathogenesis of several diseases. Aç ai has recently emerged as a natural source of antioxidants. The present study was conducted to evaluate the protective effect of Aç ai in combination with vitamin C against the aluminum chloride induced toxicity. Seventy rats were divided into 7 groups:-Group (GP) 1: control group, GP 2: treated with AlCl3, GP 3: treated with Aç ai, GP 4: treated with vitamin C, GP 5: treated with AlCl3 and Aç ai , GP 6: treated with AlCl3 and vitamin C, GP 7: treated with AlCl3, Aç ai and vitamin C. After 4 weeks, blood and liver specimens were collected to evaluate biochemical alterations and hepatic antioxidant and inflammatory parameters. AlCl3 treatment decreased liver enzymes (alanine aminotransferase, aspartate amino transferase, alkaline phosphatase), tumor necrosis factor-α and IL-6 while hepatic malondialdehyde was elevated. In contrast, hepatic glutathione, super oxide dismutase, catalase were decreased. Aç ai and vitamin C treatment improved the adverse effects induced by AlCl3, while co-administration with vitamin C promoted the action of aç ai on hepatic damage and antioxidant parameters. Aç ai showed a protective effect against AlCl3 induced toxicity, particularly in combination with vitamin C.

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“…Moreover, conflicting data have been reported by different studies on GPX expression during oxidative stress in rats with experimentally formed diabetes. For instance, both decreased [16,36] and increased [37,38] GPX enzyme expression compared to controls have been reported by separate studies in rats with STZ-induced diabetes. On the other hand, it has also been reported that, when compared with the controls, no significant difference was observed for GPX at hepatic mRNA level [39] .…”

Section: Discussionmentioning

confidence: 90%

“…This study also observed that there was no significant difference between diabetic rats and the controls with respect to GPX expression. Such different results obtained in GPX expression of diabetic rats was attributed to various experimental conditions such as age and race of the rats and the duration of the experiment [16] .…”

Section: Discussionmentioning

confidence: 99%

“…Increased lipid peroxidation and reduced glutathione levels are characteristic in diabetes [15] . Moreover, oxidative stress plays an important role in eliciting diabetic complications and underlies its pathogenesis [16] . Majority of the data suggesting a role of oxidative stress in initiating diabetes comes from animal studies that have employed alloxan and streptozotocin (STZ) to induce diabetes [10] .…”

Section: Introductionmentioning

confidence: 99%

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Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1’in Real Time-PCR ile Expresyon Profilinin Çıkarılması

Kürüm¹,

Kocamiş²,

Deprem³

et al. 2015

Kafkas Univ Vet Fak Derg

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The purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P<0.05). As a result, capsaicin causes an increase in GPX 1 gene expression at transcription level in the diabetic rat liver. Further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis. Keywords: GPX 1, RT-PCR, Capsaicin, Diabetes, Liver, Rat Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1'in Real Time-PCR ile Expresyon Profilinin Çıkarılması ÖzetBu çalışmada, Kapsaisin (CAP) uygulanan, sağlıklı ve diabetik sıçanların karaciğer dokusunda Glutatyon Peroksidaz 1' in (GPX 1) RT-PCR ile belirlenmesi amaçlanmıştır. Araştırmada 20 adet Sprague-Dawley ırkı sıçan, Diyabet (Grup I), Sadece Kapsaisin Uygulanan (Grup II), Kapsaisin uygulanmış Diabetli (Grup III) ve Kontrol (Grup IV) olmak üzere dört gruba ayrıldı. Kapsaisin uygulamasına, streptozotosin (STZ) enjeksiyonundan 72 saat sonra başlanıp, 2 hafta boyunca hem diabetik gruba hem de sadece kapsaisin uygulanan gruba her gün 1 mg/kg kapsaisin, %10 ethanol, %1 Tween 20 ve %80 distile su ile çözdürüldükten sonra subkutan olarak uygulandı. Karaciğerdeki GPX 1 geninin ekspresyonu için yapılan RT-PCR analizi sonucunda; kapsaisin uygulanan diabet grubunda GPX 1'in gen ekspresyonunun kontrol grubu da dahil diğer gruplara göre daha yüksek olduğunu belirlendi (P<0.05). Sonuç olarak diabette kullanılan kapsaisinin GPX 1 transkripsiyon artışına neden olduğu ancak protein seviyesinde bir artışa yansıyıp yansımadığının, western-blot gibi metodlarla da belirlenmesi gerektiği sonucuna varıldı.

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Dietary açai modulates ROS production by neutrophils and gene expression of liver antioxidant enzymes in rats

Cited by 66 publications

References 52 publications

Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers

Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers

Protective Effects of Açai in Combination with Vitamin C against Aluminum-Induced Toxicity in Rat Liver

Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1’in Real Time-PCR ile Expresyon Profilinin Çıkarılması

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