Dietary Administration ofAsimina triloba.(Paw Paw) Extract Increases Tumor Latency inN.-Methyl-N.-nitrosourea–Treated Rats

Cuendet, Muriel; Oteham, Carol P.; Moon, Richard C.; Keller, William J.; Peaden, Paul; Pezzuto, John M.

doi:10.1080/13880200701729497

Cited by 15 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…being marketed as beneficial for overall health (www.naturessunshine.com) and as a safe complement to cancer therapy. 8,11,26,142 Therefore, future studies must corroborate the finding of As.…”

Section: Conclusion and Future Considerationsmentioning

confidence: 92%

“…The Annonaceae, or custard-apple, family consists of many different The Annonaceae are widely studied due to their high concentrations of cytotoxic alkaloids and acetogenins, which may be useful as anti-cancer, antifungal , anti-viral, and pesticidal agents. 3,[11][12][13] Annona muricata ( Figure 3. An.…”

Section: Annonaceous Plantsmentioning

confidence: 99%

“…Contrarily, rats orally consuming pawpaw extract showed no trace of acetogenins in their blood via LC-MS/MS analysis even though the treated rats showed a delay in tumor growth compared to untreated controls. 11 Considering these limited and conflicting reports, there is a need for detailed pharmacological studies to accurately determine the bioavailability of annonacin and other acetogenins after oral ingestion in order to truly evaluate the risk of fruit or extract consumption.…”

Section: Respectively As Determined By Non-linear Regression Analysimentioning

confidence: 99%

See 2 more Smart Citations

Annonacin in Asimina triloba fruit : implications for neurotoxicity.

Potts¹

View full text Add to dashboard Cite

The acetogenin annonacin, from the tropical annonaceous plant Annona muricata, is a lipophilic, mitochondrial complex I inhibitor reported to be more toxic than rotenone to mesencephalic neurons. The temperate annonaceous plant Asimina tri/oba (pawpaw) is native to the Eastern United States and its products are available online. This study determined whether annonacin is in the pawpaw fruit pulp and whether it or the crude ethyl acetate (EtOAc) extract is toxic to cortical neurons and induces pathological changes in the microtubule associated protein tau.Pawpaw extract was prepared by pulp extraction with methanol and liquidliquid partitioning with EtOAc. Annonacin was isolated from the crude EtOAc extract via column chromatography using a gradient solvent system of increasing polarity. Mass spectroscopy, nuclear magnetic resonance and infrared v spectroscopy were used to compare isolated material with synthetic annonacin and a natural annonacin sample. Toxicity of isolated annonacin and the total EtOAc extract were determined in primary rat cortical neurons using the MIT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The effects of annonacin on tau phosphorylation were determined by immunocytochemistry and western blot analysis.The average concentration of annonacin in the fruit pulp was 0.07 ± 0.03 mg/g. Purified annonacin (30.07 !-Ig/ml) and crude EtOAc extract (47.96 !-I9/ml) reduced relative viability of cortical neurons by 50% 48 hours post treatment.Annonacin toxicity was enhanced in the presence of crude extract. Annonacin treatment resulted in a decrease in the neuronal specific marker microtubule associated protein 2 (MAP2) and tau immunoreactivity, and tau expression was decreased on western blot.In conclusion, Asimina triloba fruit contains a high concentration of annonacin, which is toxic to cortical neurons. Based on these results, annonacin acts to reduce overall levels of tau, though it remains elusive whether or not this is a direct or indirect effect of annonacin action. Crude fruit extract also induced neurotoxicity, highlighting the need for additional studies to determine the bioavailability after consumption and potential risks of neurodegeneration associated with chronic exposure to pawpaw products. vi

show abstract

Section: Conclusion and Future Considerationsmentioning

confidence: 92%

Section: Annonaceous Plantsmentioning

confidence: 99%

Section: Respectively As Determined By Non-linear Regression Analysimentioning

confidence: 99%

See 1 more Smart Citation

Annonacin in Asimina triloba fruit : implications for neurotoxicity.

Potts¹

View full text Add to dashboard Cite

show abstract

“…Acetogenins exhibit various biological activities, including antibacterial, antifungal, anticancer, and insecticidal effects (Cuendet et al., ). The anticancer activity of acetogenin has been demonstrated in various human carcinoma cell lines, including prostate, colon, liver, and gastric cancer cells (Chin, Yoon, & Kim, ), with few side effects compared to those of standard anticancer drugs (Kim & Park, ).…”

Section: Introductionmentioning

confidence: 99%

Correlation Between Acetogenin Content and Antiproliferative Activity of Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp Grown in Korea

Nam

Park

Lee

et al. 2018

Journal of Food Science

View full text Add to dashboard Cite

Pawpaw (Asimina triloba) contains acetogenins that can inhibit the growth of cancer cells. In our study, we demonstrate that the antiproliferative activity is higher in unripe than in ripe fruit and depends on acetogenin content. Our results indicate that the extract of unripe pawpaw fruit has value not only as a functional food, but has therapeutic potential for the treatment of cancer as a naturally derived substance that may be less toxic than conventional chemotherapy drugs.

show abstract

“…As McLaughlin et al did, they used three potent bioactive AGEs, bullatacin (14), asimicin (15), and trilobacin (65), in their standardised extract. Mammary tumour latency was increased from 55 to 66 days in Sprague-Dawley rats given a diet containing paw paw extract (1250 and 2500 mg/kg diet; based on maximum tolerated dose studies) [155]. In Taiwan, plants of the genus Annona are important economic crops for their edible fruits.…”

Section: Ages As Cation Ionophoresmentioning

confidence: 99%

Historic Perspectives on Annonaceous Acetogenins from the Chemical Bench to Preclinical Trials

Liaw¹,

Wu²,

Chang³

et al. 2010

Planta Med

113

View full text Add to dashboard Cite

Studies on the Annonaceous acetogenins began after the first cytotoxic acetogenin, uvaricin, was isolated in 1982. This attractive finding made many medicinal and natural product chemists direct their efforts on the isolation and identification of these classes of compounds. As more Annonaceous acetogenins were isolated, more information about them was uncovered. From their structural identification to the total synthesis of natural product analogues and from cell-based screening and molecular-based targeting to animal testing, the mechanisms of action of the Annonaceous acetogenins became clearer. The purpose of this review is to give an account of recent studies on this class of compounds and their analogues, which will aid us not only in clarifying how the Annonaceous acetogenins act but also in establishing principles for the further development of this class of compounds

show abstract

Dietary Administration ofAsimina triloba.(Paw Paw) Extract Increases Tumor Latency inN.-Methyl-N.-nitrosourea–Treated Rats

Cited by 15 publications

References 24 publications

Annonacin in Asimina triloba fruit : implications for neurotoxicity.

Annonacin in Asimina triloba fruit : implications for neurotoxicity.

Correlation Between Acetogenin Content and Antiproliferative Activity of Pawpaw (Asimina triloba [L.] Dunal) Fruit Pulp Grown in Korea

Historic Perspectives on Annonaceous Acetogenins from the Chemical Bench to Preclinical Trials

Contact Info

Product

Resources

About