Dietary administration of silk microparticles improves the epidermal and dermal regeneration after a skin wounding in gilthead seabream (Sparus aurata L.)

Albaladejo-Riad, Nora; Espinosa, Cristóbal; Esteban, María Ángeles

doi:10.1016/j.fsi.2022.03.049

Cited by 7 publications

(14 citation statements)

References 76 publications

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“…These results suggest that SF was able to enhance one of the major cellular immune responses carried out by leucocytes, phagocytosis. These results agree with previous reports on different animals in which it was demonstrated that ingestion of SF improves their immune status (Albaladejo‐Riad et al, 2022; Diez‐Echave et al, 2021; Zhang et al, 2017). The HKLs respiratory burst and peroxidase activity decreased in the wounded fish, although the respiratory burst of wounded fish fed the SF100 diet did not differ from that of non‐wounded fish.…”

Section: Discussionsupporting

confidence: 93%

“…As for the phagocytic capacity of leucocytes, it was significantly increased because of the wound. As previously reported, a skin wound can affect all fish, increasing the gene expression of many proinflammatory factors (such as interleukin‐1β, interleukin‐6, interleukin‐8 and tumoral necrosis factor‐α), which could explain this effect (Albaladejo‐Riad et al, 2022; Chen et al, 2020a). Concomitantly, leucocytes from fish fed the SF microparticle‐enriched diets showed significantly increased phagocytic capacity compared to those fed the control diet.…”

Section: Discussionmentioning

confidence: 86%

“…However, few studies have focused on the changes produced by wounds not only in the skin mucus but also in other parts of the body. Our group has shown that experimental wounds like those performed on the fish used in this work, and performed on healthy fish, are sufficient to produce remarkable changes in humoral immune parameters, not only in the skin mucus (Albaladejo‐Riad et al, 2022) but also in the serum of gilthead seabream. More specifically, the presence of a wound significantly altered the levels of IgM, proteases, antiprotease, peroxidase and lysozyme and bactericidal activity against Vibrio harveyi and Photobacterium damsealae in skin mucus.…”

Section: Discussionmentioning

confidence: 90%

“…Our research team has recently shown that administering silk microparticles in the diet enhances the regeneration of both the epidermal and dermal layers in the skin of wounded gilthead seabream ( Sparus aurata L.) (Albaladejo‐Riad et al, 2022). Based on the aforementioned findings, our group embarked on an investigation to assess the impact of these same microparticles on the cellular immunity and liver health of seabream, both with and without skin wounds.…”

Section: Introductionmentioning

confidence: 99%

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Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Albaladejo‐Riad,

Espinosa‐Ruiz,

Esteban

2024

Animal Physiology Nutrition

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Silk fibroin (SF) microparticles were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercially available diet was enriched with varying amount of SF: 0, 50 and 100 mg kg−1 (representing the control, SF50 and SF100 diets respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed on the rest of fish, and they continued to be fed the same diet. At 7 days post‐wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularisation and congestion of blood vessels). In addition, it influences the expression of genes that serve as markers of oxidative stress, endoplasmic reticulum stress and apoptosis. The highest dose of SF (SF100) increased the phagocytic capacity of leucocytes the most, as well as the expression of genes related to blood vessel formation in the liver. Furthermore, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Albaladejo‐Riad,

Espinosa‐Ruiz,

Esteban

2024

Animal Physiology Nutrition

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“…According to relevant studies, adding SF to the diet of gilthead seabream can improve wound healing and increase the expression of important genes involved in tissue repair and extracellular matrix formation. SF is considered an appropriate feed additive [116]. Zinc oxide NPs have been extensively studied as antibacterial agents and animal feed additives in livestock production [117].…”

Section: Nano-feedmentioning

confidence: 99%

Applications and Potentials of a Silk Fibroin Nanoparticle Delivery System in Animal Husbandry

Guo,

Awais,

Fei

et al. 2024

Animals

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Silk fibroin (SF), a unique natural polymeric fibrous protein extracted from Bombyx mori cocoons, accounts for approximately 75% of the total mass of silk. It has great application prospects due to its outstanding biocompatibility, biodegradability, low immunogenicity, and mechanical stability. Additionally, it is non-toxic and environmentally friendly. Nanoparticle delivery systems constructed with SF can improve the bioavailability of the carriers, increase the loading rates, control the release behavior of the deliverables, and enhance their action efficiencies. Animal husbandry is an integral part of agriculture and plays a vital role in the development of the rural economy. However, the pillar industry experiences a lot of difficulties, like drug abuse while treating major animal diseases, and serious environmental pollution, restricting sustainable development. Interestingly, the limited use cases of silk fibroin nanoparticle (SF NP) delivery systems in animal husbandry, such as veterinary vaccines and feed additives, have shown great promise. This paper first reviews the SF NP delivery system with regard to its advantages, disadvantages, and applications. Moreover, we describe the application status and developmental prospects of SF NP delivery systems to provide theoretical references for further development in livestock production and promote the high-quality and healthy development of animal husbandry.

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Effects of Juniperus root extract on wound healing, gene expression, and biochemical response in yellowtail acei Pseudotropheus sp. fish

Önalan,

Kankaya

2024

Aquacult Int

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Injuries are an undesirable situation in fish kept in aquariums. The use of antibiotics in wound healing is not preferred due to the development of antibiotic resistance in bacteria and for economic reasons. This study was conducted to demonstrate the efficacy of Juniperus root extract in healing experimentally induced incised wounds in yellowtail acei. Wound closure time, morphology, malondialdehyde content, glutathione s-transferase activity, changes in gene expression levels, non-dispersibility of the extract in aqueous environment, and retention on the wound were analyzed. In the antibiotic group, the scarring of the wound was observed on the sixth day. On the other hand, in the root extract group, wound healing was completed on the second day. In addition, a significant decrease in malondialdehyde and glutathione s-transferase levels was observed in the extract group compared to the control group. It was found that SWS2a, RH2B, and LWS gene expression levels started to decrease during wound healing at the end of the second day in the extract-treated group. In the antibiotic group, the same effect was observed on the seventh day. Important properties of the extract used, such as not dispersing in the water environment and remaining on the wound, were also determined. It would be useful to continue the experiments with Juniperus root extract, which has been shown to be effective in wound healing, with microscopic studies on aquaculture and diseases.

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Dietary administration of silk microparticles improves the epidermal and dermal regeneration after a skin wounding in gilthead seabream (Sparus aurata L.)

Cited by 7 publications

References 76 publications

Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries

Applications and Potentials of a Silk Fibroin Nanoparticle Delivery System in Animal Husbandry

Effects of Juniperus root extract on wound healing, gene expression, and biochemical response in yellowtail acei Pseudotropheus sp. fish

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