Dietary aluminosilicate supplement enhances immune activity in mice and reinforces clearance of porcine circovirus type 2 in experimentally infected pigs

Jung, Barbara; Toan, Nguyen Tat; Cho, Sun-Ju; Ko, Jae-Hyung; Jung, Yeon-Kwon; Lee, Bong‐Joo

doi:10.1016/j.vetmic.2009.11.009

Cited by 43 publications

(52 citation statements)

References 30 publications

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“…Serum was obtained by centrifugation at 2,000 × g for 10 min at 4 o C, and the separated serum was inactivated at 56 o C for 30 min. Antibody production level was measured by ELISA as previously described [17] with some modifications. Briefly, 96-well plates (Iwaki) were coated with 100 µl of a solution containing 20 µg whole formalin-killed P. multocida type A (used as antigen) in 1 ml of 0.1 M carbonate-bicarbonate buffer (pH 9.6) and left overnight at 4 o C. After three washes with PBS containing 0.05% Tween 20 (PBS-T), the wells were saturated with 200 µl of 5% skim milk (BD Biosciences).…”

Section: Methodsmentioning

confidence: 99%

“…Hence, there is an urgent need to identify ecofriendly feed supplements to reduce antibiotic use. One of the most promising methods of reducing antibiotics in industrial animals is strengthening the defense mechanisms of animals through prophylactic administration of natural immunostimulants including probiotics, clay minerals and herbs [15,17,24]. Immunostimulants increase resistance to infectious diseases by enhancing nonspecific immunity [12].…”

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confidence: 99%

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Dietary Supplementation with a Probiotic Fermented Four-Herb Combination Enhances Immune Activity in Broiler Chicks and Increases Survivability against Salmonella Gallinarum in Experimentally Infected Broiler Chicks

Jung

Lee

2010

The Journal of Veterinary Medical Science

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ABSTRACT. Herbs including Curcuma longa, Houttuynia cordata, Prunus mume and Rubus coreanus have potential immune enhancing and antimicrobial effects. Probiotics also have antibacterial effects, and some are important in regulating the immune system. The aims of the present study were to evaluate the immune enhancing effects of a probiotic fermented four-herb combination (PFH) in broiler chicks and to demonstrate the prophylactic effect of PFH against Salmonella Gallinarum in experimentally infected broiler chicks as an initial step towards the development of feed supplements for promotion of immune activity and disease prevention. Continuous ingestion of PFH markedly increased lysozyme activity in serum and the spleen, peripheral blood mononuclear cell (PBMC) proliferation, the CD4 + :CD8 + T lymphocyte ratio in the spleen and antibody production level in broiler chicks. Conversely, prostaglandin E 2 synthesis in serum and PBMC culture medium was significantly decreased in the PFH-fed chicks compared with the control group in a dose-dependent manner. In the chicks experimentally infected with S. Gallinarum, mortality was delayed in the 2% PFH-fed chicks. Moreover, the survival rates in the 2% PFH-fed group remained the highest among all the trial groups throughout the experimental period. Taken together, these findings suggest that PFH enhances immune activity in broiler chicks and increases survivability against Salmonella Gallinarum in experimentally infected broiler chicks, likely because of potent stimulation of nonspecific immune responses.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Dietary Supplementation with a Probiotic Fermented Four-Herb Combination Enhances Immune Activity in Broiler Chicks and Increases Survivability against Salmonella Gallinarum in Experimentally Infected Broiler Chicks

Jung

Lee

2010

The Journal of Veterinary Medical Science

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“…However, only a small number of these have been shown to be effective. Amongst the vast variety of alternatives to dietary AGP tested, clinoptilolite (CPL), which is the most common member of the naturally occurring zeolite family of crystalline hydrated aluminosilicate minerals (Mumpton 1999), has shown promise as a growthpromoting (Defang and Nikishov 2009;Prvulovic et al 2012), immune enhancing/antiviral (Jung et al 2010) and gut health restoring (Vondruskova et al 2010;Hrenovic et al 2012) antibacterial dietary supplement in pigs. However, studies reported thus far have shown inconsistent results, suggesting that the reported potential of dietary CPL may have certain limitations due to the lack of standardisation of its physicochemical properties (especially particle size), and the heterogeneity of rearing conditions, e.g.…”

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In-feed supplementation of clinoptilolite favourably modulates intestinal and systemic immunity and some production parameters in weaned pigs

Valpotić¹,

Terzić²,

Vince³

et al. 2016

Vet. Med.

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ABSTRACT:The ban on dietary antibiotic growth promoters (AGP) in swine production has focused increasing research efforts on the development of alternative feed supplements. One such alternative to AGP is the dietary zeolite clinoptilolite (CPL). The aims of this study were to evaluate, in weaned pigs, the effects of CPL on: (a) growth performance, (b) gut health (reduction of harmful bacteria and, incidence/severity of diarrhoea) and (c) circulating or ileal mucosal subsets of lymphoid immune cells, over the course of five weeks post weaning. The non-treated pigs received standard Phase 1 diet from Day 0 to 21 and Phase 2 diet from Day 22 to 35, whereas both diets of experimental pigs were supplemented with 0.5% CPL. The pigs receiving diet supplemented with CPL had significantly higher average daily gain at Day 28 but significantly lower daily gain at Day 35 of the experiment (P < 0.05). The CPL group exhibited non-significantly improved feed conversion ratio (1.83 vs. 2.17) for the total duration of the experiment (Day 0 to Day 35). Although shedding of haemolytic/enterotoxigenic E. coli was more frequent in the CPL group, the sum of their diarrhoea severity score was 12.96% lower (47 vs 54) than that of the non-treated controls. The proportions of circulating lymphoid cell subsets tested (CD45 + , CD4 + , CD21 + ), were significantly (P < 0.05 to P < 0.01) higher in CPL-treated pigs between Day 21 and Day 35 of the experiment. Immunohistology/ morphometry of ileal segments revealed an increased recruitment of CD45RA + cells in interfollicular (P < 0.05),but not in follicular areas of ileal PP of CPL-treated pigs at Day 35. In conclusion, CPL did not improve growth in weaned pigs, and generally it failed to improve their feed conversion efficiency. Further, it did not suppress faecal shedding of enterotoxigenic E. coli; however, it was shown to be effective as an immunomodulatory agent by promoting the recruitment of circulating and intestinal immune cell subsets.

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“…They support the immune activity [20,42,43] note that clinoptilolite with low toxicity and high adsorption capacity toward Pb 2+ in a strong acid solution has a potentiality for application in the life sciences. Clinoptilolites appears to be stable in the gastrointestinal tract [44], and as unique selective adsorbers, they could adsorb toxins, heavy metals, and free radicals from the body and excretes [17,45]. It is believed that the high affinity of clinoptilolite to Pb would significantly reduce the amount of dietary Pb available for absorption by the intestinal mucosa [20].…”

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confidence: 99%

Zeolites versus Lead Toxicity

R¹

2015

J Bioequiv Availab

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Dietary aluminosilicate supplement enhances immune activity in mice and reinforces clearance of porcine circovirus type 2 in experimentally infected pigs

Cited by 43 publications

References 30 publications

Dietary Supplementation with a Probiotic Fermented Four-Herb Combination Enhances Immune Activity in Broiler Chicks and Increases Survivability against Salmonella Gallinarum in Experimentally Infected Broiler Chicks

Dietary Supplementation with a Probiotic Fermented Four-Herb Combination Enhances Immune Activity in Broiler Chicks and Increases Survivability against Salmonella Gallinarum in Experimentally Infected Broiler Chicks

In-feed supplementation of clinoptilolite favourably modulates intestinal and systemic immunity and some production parameters in weaned pigs

Zeolites versus Lead Toxicity

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