Dietary and Endogenous Long-Chain Fatty Acids in the Intestine of Sheep, with an Appendix on Their Estimation in Feeds, Bile, and Faeces

Heath, TJ; Hill, Lyn

doi:10.1071/bi9691015

Cited by 19 publications

(18 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In sheep, diversion of bile from the intestine is followed by substantial decreases in the flow of bile [Harrison, 1962], and in the rate of secretion of total fatty acids [Heath and Hill, 1969].…”

mentioning

confidence: 99%

Effect of the Enterohepatic Circulation of Bile Salts on the Flow of Bile and Its Content of Bile Salts and Lipids in Sheep

Heath¹,

Caple²,

Redding³

1970

Exp Physiol

View full text Add to dashboard Cite

When bile was diverted from the intestine of four sheep for 12-48 hr, they secreted 0-31±0-019 ml. bile/min. This contained 5 0+0 55 ,tEq total fatty acids, principally in phosphatidylcholine (48±1.9%) and lysophosphatidylcholine (31±5-0%). The output of total fatty acids increased when taurocholate was infused into the portal vein or duodenum, and this was due to an increased secretion of phosphatidylcholine. Stimulation of total fatty acid secretion by bile salts was most marked at low rates of bile salt transfer, and reached a maximum when taurocholate was infused into the portal vein at 37 ,uM/min or more. The mean maximum output of total fatty acids in the bile of 12 sheep was 15-2±1-41 ,tM/min. When 14C-palmitic acid was infused into the portal vein with the taurocholate, it appeared in biliary phospholipids, and by the sixth hour of the infusion the level of radioactivity in bile was 8.3% of the rate of infusion. When taurocholate was infused into the portal vein at 45 pLM/min or less it was transferred quantitatively into bile, but the efficiency of transfer decreased at higher infusion rates. Secretion of bile salts into bile was accompanied by increased secretion of water, but the volume of water accompanying each micromole of bile salt decreased with increases in the rate of secretion. The bile flow did not increase further when more than 127 tM/min taurocholate was infused into the portal vein, and in four sheep the maximum rate was 1F06 ±0-052 ml./min. It is suggested that availability of bile salt may limit secretion of phospholipid into bile when the rate of secretion of bile salt is low, but that when large amounts of bile salt are secreted into bile some other factor, possibly the rate of phospholipid synthesis, limits the secretion of phospholipids into bile.

show abstract

mentioning

confidence: 99%

Effect of the Enterohepatic Circulation of Bile Salts on the Flow of Bile and Its Content of Bile Salts and Lipids in Sheep

Heath¹,

Caple²,

Redding³

1970

Exp Physiol

View full text Add to dashboard Cite

show abstract

“…Total biliary fatty acids were used as an indication of phospholipid concentration, and were estimated after hydrolysis of fatty acid esters in alkaline ethanol (Heath and Hill 1969). Radioactive nickel soaps were formed from the fatty acids with 6 3 Ni, and liquid scintillation counting used to estimate the level of radioactivity in the soaps (M. W. Simpson-Morgan, personal communication).…”

Section: Methodsmentioning

confidence: 99%

Effect of Oestradiol Benzoate on Biliary Phospholipids in the Rat

Holloway¹,

Heath²

1973

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Bile was collected from conscious, ovariectomized, young female rats which had been treated with either oestradiol benzoate or peanut oil for 3 weeks. L-[Me-14 C]-Methionine was given intravenously immediately before collection of bile was started. The bile flow increased and the concentration of biliary phospholipids decreased as a result of the oestrogen treatment, but increases occurred in the output of radioactivity in bile and in the specific activity of biliary phospholipids. It seems most likely that oestrogen treatment increased the proportion of biliary phospholipids formed by methylation of phosphatidylethanolamine.

show abstract

“…The samples of bile collected during the experiments were analysed for total fatty acids by the method tested and described by Heath and Hill [1969] and for total cholates by the method of Irvin, Johnston and Kopala [1944]. The concentration of sodium was estimated with a Baird Atomic Model KY2 Flame Photometer, and osmolality was estimated with a Fiske osmometer.…”

Section: Methodsmentioning

confidence: 99%

Effect of Secretin on Bile Formation in Sheep

Heath

1970

Exp Physiol

View full text Add to dashboard Cite

The rate of flow of bile increased rapidly when pure natural secretin was infused into the portal vein of conscious, standing sheep. The increment in flow was related significantly to the logarithm of the rate of administration but was not affected by the simultaneous infusion of sodium taurocholate into the portal vein. When secretin and taurocholate were given together the rate of flow of bile was five times that during the control period of bile salt deprivation. Secretin stimulated the secretion of total fatty acids into the bile of sheep when they were deprived of recirculated bile salts and also when they received an infusion of taurocholate into the portal vein. The mean maximum rate of total fatty acid secretion was 38 per cent higher during infusion of secretin with taurocholate than during infusion of taurocholate alone. When taurocholate was infused into the portal vein at 37 ,umole/min or less, it was transferred quantitatively into the bile, and secretin did not appear to affect this process. However, when taurocholate was infused alone at 112 ,umole/min it was not transferred quantitatively into the bile, but the efficiency of this transfer was increased by the simultaneous infusion of secretin. The concentration in the bile of total fatty acids and of bile salts decreased during infusion of secretin, but no significant changes occurred in the concentration of sodium. The infusion of secretin caused no significant changes in the osmolality of the bile in five sheep, but in three experiments on a sixth sheep it caused a highly significant increase in osmolality. When secretin was infused into the portal vein, no changes occurred in the arterial pressure, pulse rate, portal venous pressure or respiratory rate. These experiments indicate that secretin could play a significant role in regulating bile secretion in the sheep.Secretin may be an important stimulus to the secretion of water and electrolytes into the bile in carnivorous and omnivorous animals [Still, McBean and

show abstract

Dietary and Endogenous Long-Chain Fatty Acids in the Intestine of Sheep, with an Appendix on Their Estimation in Feeds, Bile, and Faeces

Cited by 19 publications

References 20 publications

Effect of the Enterohepatic Circulation of Bile Salts on the Flow of Bile and Its Content of Bile Salts and Lipids in Sheep

Effect of the Enterohepatic Circulation of Bile Salts on the Flow of Bile and Its Content of Bile Salts and Lipids in Sheep

Effect of Oestradiol Benzoate on Biliary Phospholipids in the Rat

Effect of Secretin on Bile Formation in Sheep

Contact Info

Product

Resources

About