Dietary and genetic modulation of DNA repair in healthy human adults

Tyson, John R.; Mathers, John C.

doi:10.1017/s0029665107005289

Cited by 22 publications

(6 citation statements)

References 55 publications

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“…Next to the effect of single nucleotide polymorphisms (SNP) on DNA-repair activity, also other factors, such as diet and specific dietary compounds, are thought to modulate DNA-repair capacities. Although there is sufficient evidence for chemopreventive effects of certain dietary compounds ( 19 ) , only a few studies have reported that dietary compounds influence DNA-repair processes (for a review, see Tyson & Mathers ( 20 ) ). Several of these studies investigated the dietary modulation of BER or the repair of oxidative lesions.…”

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confidence: 99%

Modulation of nucleotide excision repair in human lymphocytes by genetic and dietary factors

et al. 2009

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Gene-environment interactions determine inter-individual variations in nucleotide excision repair (NER) capacity. Oxidative stress was previously found to inhibit NER, thus supplementation with dietary antioxidants could prevent this inhibition, especially in genetically susceptible subjects. To study the effects of genetic polymorphisms in NER-related genes and dietary intake of antioxidants on an individual's NER capacity, lymphocytes of 168 subjects were isolated before and after a 4-week blueberry and apple juice intervention. Twelve genetic polymorphisms in NER genes XPA, XPC, ERCC1, ERCC2, ERCC5, ERCC6 and RAD23B were assessed by multiplex PCR with single base extension. Based on specific genotype combinations, a subset of individuals (n 36) was selected for phenotypical assessment of NER capacity, which was significantly affected by the total sum of low-activity alleles (P¼ 0·027). The single polymorphism XPA G23A was the strongest predictor of NER capacity (P¼ 0·002); carriers of low-activity alleles AA had about three times lower NER capacity than XPA GG carriers. NER capacity assessed before and after intervention correlated significantly (R 2 0·69; P,0·001), indicating that inter-individual differences in NER capacity are maintained over 4 weeks. Although the intervention increased plasma trolox equivalent antioxidant capacity from 791 (SE 6·61) to 805 (SE 7·90) mM (P¼ 0·032), on average it did not affect NER capacity. Nonetheless, carriers of twelve or more low-activity alleles seemed to benefit from the intervention (P¼ 0·013). Among these, carriers of the variant allele for RAD23B Ala249Val showed improved NER capacity upon intervention (P¼ 0·020). In conclusion, improved NER capacity upon dietary intervention was detected in individuals carrying multiple low-activity alleles. The XPA G23A polymorphism might be a predictor for NER capacity.

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Modulation of nucleotide excision repair in human lymphocytes by genetic and dietary factors

et al. 2009

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“…This enhancement is correlated with the upregulation of some critically important proteins that govern the NER process ( Figure 9 ). Nevertheless, it is also important to recognize that there is a wide inter-individual variation in DNA repair capacities which could be due to polymorphisms in the genes encoding DNA repair proteins [ 46 ] However, BRBE had a much smaller impact on the BER pathway using Gh as the substrate. Despite its weak effect on BER activity, anthocyanins in BRB have been shown to scavenge reactive oxygen species (ROS) and thus protect cells from oxidative stress and DNA damage [ 47 ].…”

Section: Perspectivesmentioning

confidence: 99%

Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism

et al. 2022

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As demonstrated by us earlier and by other researchers, a diet containing freeze-dried black raspberries (BRB) inhibits DNA damage and carcinogenesis in animal models. We tested the hypothesis that the inhibition of DNA damage by BRB is due, in part, to the enhancement of DNA repair capacity evaluated in the human HeLa cell extract system, an established in vitro system for the assessment of cellular DNA repair activity. The pre-treatment of intact HeLa cells with BRB extracts (BRBE) enhances the nucleotide excision repair (NER) of a bulky deoxyguanosine adduct derived from the polycyclic aromatic carcinogen benzo[a]pyrene (BP-dG) by ~24%. The NER activity of an oxidatively-derived non-bulky DNA lesion, guanidinohydantoin (Gh), is also enhanced by ~24%, while its base excision repair activity is enhanced by only ~6%. Western Blot experiments indicate that the expression of selected, NER factors is also increased by BRBE treatment by ~73% (XPA), ~55% (XPB), while its effects on XPD was modest (<14%). These results demonstrate that BRBE significantly enhances the NER yields of a bulky and a non-bulky DNA lesion, and that this effect is correlated with an enhancement of expression of the critically important NER factor XPA and the helicase XPB, but not the helicase XPD.

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“…Macronutrient (carbohydrate, protein and lipid) deficiencies are also associated with DNA damage. For instance, severe protein calorie deficiency and excessive calorie intake, which lead to overweight/ obesity, are also associated with DNA damage (19,20). From this point of view, preservation of nutrition sheds light on that DNA damage and circadian rhythm regulation but the effect of these three events on each other is remains unexplained.…”

Section: Our Cells Have Complex Dna Repair To Protectmentioning

confidence: 99%

“…In cell metabolism, DNA repair, mRNA, tRNA, rRNA synthesis, protein and other biomolecules are possible by the presence of sufficient amount and variety of nutrients in the cytoplasm. Tyson and Mathers reported the amount and variety of nutritional support affecting DNA repair in malnourished individuals (20). There are many bioactive components that repair DNA in fruits and vegetables (33).…”

Section: Dna Repair and Nutrition Biochemistrymentioning

confidence: 99%

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The circadian rhythm works as a clock that regulates many physiological processes including metabolism, sleep, neurobehavior, epigenetic and hormone secretion so that disruption of the circadian rhythm causes adverse effects on human health. The mutations in DNA increase the risk of developing cancer (a genetic disease caused by genome damage). Cancer formation is also caused by disruption of the circadian rhythm. Therefore, a cell's ability to properly respond to DNA damage and repair DNA is critical in preventing cancer formation. Prevention of DNA damage is important for the occurrence of cancer. Epidemiological and experimental evidence explain the variation of dietary intake effects in cancer prevalence. Dietary factors may affect the efficiency of DNA repair, their effectiveness on epigenetic mechanisms, as well as circadian rhythm and inhibit cancer formation. The purpose of this review is to reveal the effects of nutrition on circadian rhythm and DNA repair, emphasize the scarcity of existing studies, and pave the way for possible experimental studies. There are very few studies in this field, and the future studies of nutrients that affect circadian rhythm are very valuable in order to find new solutions in cancer treatment. Nutrition may have many effects on the circadian system and DNA repair, and ultimately help reduce the burden of chronic diseases.

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Dietary and genetic modulation of DNA repair in healthy human adults

Cited by 22 publications

References 55 publications

Modulation of nucleotide excision repair in human lymphocytes by genetic and dietary factors

Modulation of nucleotide excision repair in human lymphocytes by genetic and dietary factors

Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism

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