Dietary Beta-Hydroxy-Beta-Methyl Butyrate Supplementation Inhibits Hepatic Fat Deposition via Regulating Gut Microbiota in Broiler Chickens

Zhang, Shiyu; Tang, Zhiyi; Zheng, Chunyan; Zhong, Yinzhao; Zheng, Jie; Duan, Geyan; Yin, Yulong; Duan, Yehui; Song, Zehe

doi:10.3390/microorganisms10010169

Cited by 12 publications

(16 citation statements)

References 43 publications

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“…An interesting finding from the present study was that crude lipid content in shrimp muscle significantly decreased in response to dietary HMB supplementation, which may partially contribute to the improved muscle firmness of the HMB2 group. Previous studies reported that dietary HMB could decrease fat deposition in Bama Xiang mini-pigs [ 63 ] and broiler chickens [ 64 ] through regulating the Bacteroidetes -acetic acid-AMPK α axis and gut microbiota respectively, which needs to be further investigated and verified in aquatic animals.…”

Section: Discussionmentioning

confidence: 99%

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Mao¹,

Yang²,

Chen³

et al. 2023

Aquaculture Nutrition

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An 8-week feeding trial was performed to evaluate the effects of dietary β-hydroxy-β-methylbutyrate (HMB) supplementation on growth performance and muscle quality of kuruma shrimp (Marsupenaeus japonicas) (initial weight: 2.00 ± 0.01 g ) fed a low protein diet. The positive control diet (HP) with 490 g/kg protein and negative control diet (LP) with 440 g/kg protein were formulated. Based on the LP, 0.25, 0.5, 1, 2 and 4 g/kg β-hydroxy-β-methylbutyrate calcium were supplemented to design the other five diets named as HMB0.25, HMB0.5, HMB1, HMB2 and HMB4, respectively. Results showed that compared with the shrimp fed LP, the HP, HMB1 and HMB2 groups had significantly higher weight gain and specific growth rate, while significantly lower feed conversion ratio ( p < 0.05 ). Meanwhile, intestinal trypsin activity was significantly elevated in the above three groups than that of the LP group. Higher dietary protein level and HMB inclusion upregulated the expressions of target of rapamycin, ribosomal protein S6 kinase, phosphatidylinositol 3-kinase, and serine/threonine-protein kinase in shrimp muscle, accompanied by the increases in most muscle free amino acids contents. Supplementation of 2 g/kg HMB in a low protein diet improved muscle hardness and water holding capacity of shrimp. Total collagen content in shrimp muscle increased with increasing dietary HMB inclusion. Additionally, dietary inclusion of 2 g/kg HMB significantly elevated myofiber density and sarcomere length, while reduced myofiber diameter. In conclusion, supplementation of 1-2 g/kg HMB in a low protein diet improved the growth performance and muscle quality of kuruma shrimp, which may be ascribed to the increased trypsin activity and activated TOR pathway, as well as elevated muscle collagen content and changed myofiber morphology caused by dietary HMB.

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Section: Discussionmentioning

confidence: 99%

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Mao¹,

Yang²,

Chen³

et al. 2023

Aquaculture Nutrition

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“…Thus, it is urgent to seek drugs or feed additives to improve fat metabolism in laying hens. While HMB, a metabolite of L-leucine in the liver, was recently reported to modulate hepatic lipid metabolism in broiler chickens [ 22 ], it is still unknown whether dietary supplementation with HMB could alleviate fat over-accumulation in laying hens. This is because there are huge differences between broiler chickens and layer chickens in terms of genetics, nutrition, physiology, and feeding management [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Frankly, this is an expected finding because the gut microbiota is well-known for its broad range of roles in the regulation of many physiological and pathological processes, particularly metabolism [ 46 ]. In addition, studies in mice and broilers have already indicated that the gut microbiota plays a role in the lipid-lowering action of HMB [ 20 , 22 ]. However, until now, it has been unclear how HMB shapes the gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

“…Although our work has demonstrated that dietary supplementation with HMB facilitates lipid catabolism and protects layers against fat over-accumulation, it is still unknown whether HMB improves metabolic health, egg quality, as well as egg production in laying hens under standard poultry farming conditions. Furthermore, it should be noted that the beneficial roles of HMB are highly dose- and species-dependent and that lower doses seem to have better effects than higher doses [ 19 , 20 , 22 ]. However, the optimal doses of HMB for different species of birds and animals are as yet undetermined.…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, recent studies have indicated that low-dose dietary HMB supplementation alleviates dorsal subcutaneous fat deposition in pigs and global obesity in high-fat diet (HFD)-induced obese mice though remodeling of gut microbiota composition and function, especially Bacteroidetes −mediated SCFA production [ 19 , 20 ]. Moreover, studies on broiler chickens have revealed that 0.10% HMB improves growth performance, meat quality, and hepatic lipid accumulation [ 21 , 22 ]. However, until now, there have been no reports regarding HMB in laying hens.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens

Liao

et al. 2022

CIMB

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Excessive lipid deposition in layer chickens due to inappropriate feeding adversely affects egg production; however, nutritional manipulation methods to deal with this issue are still limited. β-hydroxy-β-methylbutyrate (HMB), a metabolite of L-leucine, was recently reported as a lipid-lowering nutrient in mice and pigs, although its role in layers had not been investigated. Here, we employed high-fat and high-cholesterol diet (HFHCD)−challenged growing layers as an obese model to explore HMB function in the regulation of lipid metabolism and the potential mechanisms involved. We found that dietary supplementation with (0.05% or 0.10%) HMB significantly reduced HFHCD−induced bodyweight growth in layers, mainly due to reduction in abdominal fat deposition. Mechanistically, HMB supplementation enhanced hepatic bile acid synthesis from cholesterol through elevating expression of Cyp7a1, a gene coding a key enzyme in bile acid synthesis. Furthermore, 16S rRNA gene sequencing revealed that HMB supplementation remodeled the diversity and composition of the layers’ cecal microbiota, and the abundance of Bacteroidetes at the phylum level were especially affected. Correlation analysis further indicated a strong negative association between Bacteroidetes abundance and lipid metabolism−related parameters. Taken together, these data suggest that dietary HMB supplementation could improve abdominal fat deposition in layers, probably through modulating hepatic bile acid synthesis and gut microbiota function.

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Beta‐hydroxy‐β‐methyl butyrate‐supplemented diet for broiler chickens is more conducive to dietary protein reduction than a leucine‐supplemented diet until 21 days old

Kop Bozbay,

Yılmaz,

Ocak

2023

J Sci Food Agric

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BACKGROUNDDietary L‐leucine or its metabolite 𝛽‐hydroxy‐𝛽‐methylbutyrate (HMB) has a crucial role in the muscle protein metabolism of broilers during the first few‐week growing period. This study aimed to evaluate the effects of L‐leucine (LLPD) or HMB (HLPD) supplementation in a low‐protein diet (20%, LPD) until 21 days old on performance, carcass weight, muscle yield, and meat quality, as well as intestinal morphometry in broiler chickens.RESULTSFrom d 1 to 42, LPD decreased body weight gain (BWG) and feed intake (FI) and increased feed conversion ratio (FCR) compared to a standard protein diet (22%, SPD). The LLPD and HLPD did not affect FI, but the LLPD decreased the BWG and increased the FCR compared to the SPD. The LPD group had lower body and muscle weights than other groups. Compared to LPD, HLPD increased dressing percentage. The LPD decreased the serum IGF‐1 content compared to the SPD and LLPD. The duodenal villus height of the LPD and LLPD broilers was smaller than those of the SPD and HLPD birds. The HLPD broilers had lower duodenal villus width than the SPD birds. The duodenal crypt depth and ileal mucosal thickness were higher in the HLPD group than in other groups. The HLPD and LLPD enhanced the ileal villus height compared to the SPD. The LLPD and HLPD treatments did not affect meat quality traits compared to the SPD treatment.CONCLUSIONDietary HMB could be a conducive approach to reducing dietary protein for broilers until 21 days old.This article is protected by copyright. All rights reserved.

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Dietary Beta-Hydroxy-Beta-Methyl Butyrate Supplementation Inhibits Hepatic Fat Deposition via Regulating Gut Microbiota in Broiler Chickens

Cited by 12 publications

References 43 publications

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Dietary β-Hydroxy-β-Methylbutyrate Supplementation Affects Growth Performance, Digestion, TOR Pathway, and Muscle Quality in Kuruma Shrimp (Marsupenaeus japonicas) Fed a Low Protein Diet

Effects of Dietary Inclusion of β-Hydroxy-β-Methylbutyrate on Growth Performance, Fat Deposition, Bile Acid Metabolism, and Gut Microbiota Function in High-Fat and High-Cholesterol Diet-Challenged Layer Chickens

Beta‐hydroxy‐β‐methyl butyrate‐supplemented diet for broiler chickens is more conducive to dietary protein reduction than a leucine‐supplemented diet until 21 days old

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