Dietary bovine lactoferrin induces changes in immunity level and disease resistance in Asian catfish Clarias batrachus

Kumari, Jaya; Swain, T.; Sahoo, P.K.

doi:10.1016/s0165-2427(03)00065-5

Cited by 89 publications

(94 citation statements)

References 32 publications

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“…γ-globulins in LF group showed insignificant differences comparing with control group. This result was the same with Jaya and Shaoo [27] who reported that oral feeding of LF potentially stimulate non-specific immune response while specific immunity was not influenced by LF feeding.…”

Section: Groups T Protein Gm/dl Albumin Gm/dl α-Globulin Gm/dl β-Glosupporting

confidence: 90%

Studies on the Immunomodulatory Effects of Lactoferrin in Rats Infected with E. coli

El‐Boshy¹,

Abdalla²,

Hassan³

2013

JIBTVA

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Eighty male albino rats of Westar strain (350 ± 10 g), 10 to 12 weeks old were divided into four groups. The groups treated are as following. The first control group (Gp. I) was given intraperitoneally in normal saline (1 mL). The second group (Gp. II) was orally infected with 3 × 10 12 CFU of E. coli /Kg. BW. The third group (Gp. III) was infected with E. coli and treated with (0.5%) lactoferrin (LF) 72 hours before E. coli infection in filtered tap water for the duration of the experiment (21 days). The fourth group (Gp. IV) was administrated with LF only (0.5%) in drinking water. Two separate blood samples were collected from heart puncture at the end of 1 st , and 3 rd week post-treatment for immunological studies. The Leukogram in E. coli treated group was insignificant compared with the control group while lymphocytosis was clear compared with the infected group. Total protein, albumin, α-globulin and β-globulin were insignificantly changed in LF & E. coli treatment group comparing with infected and control groups. TNF-α and γ-globulin are significantly increased in infected group comparing with other treated groups. In conclusion, lactoferrin has powerful antibacterial activity in a variety of ways as well as a safe immunostimulant protein when it is orally administrated.

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Section: Groups T Protein Gm/dl Albumin Gm/dl α-Globulin Gm/dl β-Glosupporting

confidence: 90%

Studies on the Immunomodulatory Effects of Lactoferrin in Rats Infected with E. coli

El‐Boshy¹,

Abdalla²,

Hassan³

2013

JIBTVA

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“…46 Thus, it is possible that the anabolic actions of lactoferrin might continue into the neonatal period. In adult animals, although some groups studied the biological effects of orally administered lactoferrin, [47][48][49] the effect on bone formation has not been determined. The main source of lactoferrin in the circulation of the adult is the secondary granules of neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Lactoferrin - A Novel Bone Growth Factor

Naot¹,

Grey²,

Reid³

et al. 2005

Clinical Medicine & Research

148

101

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Lactoferrin is an iron-binding glycoprotein that belongs to the transferrin family. It is present in breast milk, in epithelial secretions, and in the secondary granules of neutrophils. In healthy subjects lactoferrin circulates at concentrations of 2-7 x 10 -6 g/ml. Lactoferrin is a pleiotropic factor with potent antimicrobial and immunomodulatory activities. Recently, we have shown that lactoferrin can also promote bone growth. At physiological concentrations, lactoferrin potently stimulates the proliferation and differentiation of primary osteoblasts and also acts as a survival factor inhibiting apoptosis induced by serum withdrawal. Lactoferrin also affects osteoclast formation and, in murine bone marrow culture, lactoferrin potently inhibits osteoclastogenesis. In vivo, local injection of lactoferrin above the hemicalvaria of adult mice results in substantial increases in the dynamic histomorphometric indices of bone formation and bone area.The mitogenic effect of lactoferrin in osteoblast-like cells is mediated mainly through LRP1, a member of the family of low-density lipoprotein receptor-related proteins that are primarily known as endocytic receptors. Using confocal laser scanning microscopy, we demonstrated that fluorescently labeled lactoferrin is endocytosed and can be visualized in the cytoplasm of primary osteoblastic cells. Lactoferrin also induces activation of p42/44 MAPK signaling in primary osteoblasts, but the two pathways seem to operate independently as activation of MAPK signaling, but not endocytosis, is necessary for the mitogenic effect of lactoferrin. We conclude that lactoferrin may have a physiological role in bone growth and healing, and a potential therapeutic role as an anabolic factor in osteoporosis.

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“…Similar attempts have been made to counteract natural environmental pollutants, viz. organophosphorus and organochlorine pesticides, through the administration of chitosan, Finnstim, dimerised lysozyme and vitamin C in fish (Siwicki 1989, Dunier et al 1995.Previous findings showed that administration of dietary bovine lactoferrin, glucan, levamisole and vitamin C increased resistance to different bacterial diseases caused by Aeromonas sp., Vibrio sp., Edwardsiella sp., Yersinia ruckeri and Ichthyophthirius multifiliis in different freshwater and marine fish species (as reviewed by Mulero et al 1998b, Sakai 1999, Sahoo & Mukherjee 2001a,b, 2002, Kumari et al 2003, Kumari & Sahoo 2005b.The present study evaluated the comparative influence of the dietary administration of the 4 proven fish immunostimulants in healthy and CYP-induced immunocompromised Asian catfish by screening specific immunity against Aeromonas hydrophila bacterin and disease resistance after challenge with a virulent strain of A. hydrophila. …”

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confidence: 99%

“…The basal or control feed mix contains: fish meal, 35%; groundnut cake, 25%; soybean meal, 20%; rice bran, 12%; wheat flour, 5%; carboxy methyl cellulose, 1%; and vitamin-mineral mix, 2% (as per Kumari & Sahoo 2005b). Based on our previous studies (Kumari et al 2003, Kumari & Sahoo 2005b, immunostimulants were added individually to the control diet. The doses and durations of feeding for immunostimulants are as follows: lactoferrin, 100 mg kg -1 feed for 7 d; β-1, 3 glucan, 0.1% in feed for 7 d; levamisole, 50 mg kg -1 feed for 10 d; and vitamin C, 500 mg kg -1 feed for 30 d. The immunostimulant-supplemented pelleted feeds were stored at -20°C after air drying, except for vitamin C, which was prepared weekly and stored at -20°C.…”

mentioning

confidence: 99%

“…Subgroups a1 to e1 were treated as non-vaccinated healthy animals, whereas fish of Subgroups a2 to e2 were injected with CYP i.p. on Days 26, 27 and 28 in a previously described manner ( All fish were anaesthetised with a sedative dose of 2-phenoxyethanol (Sigma) (100 l phenoxyethanol 1 -1 water) (Kumari et al 2003) prior to any injection or blood collection from the caudal vein, to minimise the possible stress induced during handling.Bacterin preparation and vaccination. Formalinkilled Aeromonas hydrophila bacterin was prepared according to Kumari et al (2003), and 0.1 ml of suspension was injected i.p.…”

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confidence: 99%

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Dietary immunostimulants influence specific immune response and resistance of healthy and immunocompromised Asian catfish Clarias batrachus to Aeromonas hydrophila infection

Kumari

Sahoo²

2006

Dis. Aquat. Org.

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In order to determine the efficacy and immunoreversal effect of the 4 dietary immunomodulators, viz. lactoferrin, β-1, 3 glucan, levamisole and vitamin C, on disease resistance of a commercially important catfish, Clarias batrachus, fish were fed diets supplemented with various levels of these substances in 2 subgroups, healthy and immunocompromised, during a 30 d trial. An artificial immunosuppressive state was induced by giving 3 intraperitoneal (i.p.) injections of cyclophosphamide (CYP) at a dose level of 200 mg kg -1 body weight at 1 wk intervals in the immunocompromised vaccinated subgroup and 3 consecutive injections 3 d before challenge in the immunocompromised non-vaccinated subgroup. On the first day of the experiment, the fish were vaccinated against a formalin-killed Aeromonas hydrophila bacterin. After 30 d, antibody titre (as measured through bacterial agglutination titre) and disease resistance against A. hydrophila were determined. The results demonstrate that all 4 immunomodulators were capable of significantly (p < 0.05) enhancing the specific immune response; this was evident through raised antibody titre and protection against A. hydrophila in both healthy and immunocompromised vaccinated subgroups compared to their respective controls. Similarly, all 4 substances significantly raised the survival rates in immunocompromised and healthy non-vaccinated fish. Thus, these substances were capable of reducing the immunosuppression induced by CYP injections in both vaccinated and non-vaccinated fish compared to their respective controls. Among the 4 substances studied, β-1, 3 glucan was found to be the most effective immunomodulator, followed by levamisole, lactoferrin and vitamin C in Asian catfish. Therefore, the results support the introduction of these substances into the diet of fish grown in farms under immunosuppressive/stressful conditions in order to enhance protection against infection and offer economic benefits. KEY WORDS: Immunomodulators · Cyclophosphamide · Specific immunity · Disease resistance · Clarias batrachus · Aeromonas hydrophila Resale or republication not permitted without written consent of the publisherDis Aquat Org 70: [63][64][65][66][67][68][69][70] 2006 suppressant in Clarias batrachus. Previous studies have shown that low levels of CYP exposure in fish, though not lethal, cause marked immunosuppression (Chen et al. 1983, Kitao & Yoshida 1986, Kumari & Sahoo 2005a.Aeromonas hydrophila, a ubiquitous bacterium responsible for stress-associated pathogenicity in warmwater fish (e.g. carp, catfish and salmonids) is associated with small surface lesions, local haemorrhaging and septicaemia (Yin et al. 1996). Different vaccination strategies have been employed with different bacterin preparations against A. hydrophila, inducing protection against aeromoniasis in carp and catfish species with varying degrees of success (Yin et al. 1996, Nayak et al. 2004. No commercial vaccine against A. hydrophila is currently available for Asian catfish farming.Recent studies of...

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Dietary bovine lactoferrin induces changes in immunity level and disease resistance in Asian catfish Clarias batrachus

Cited by 89 publications

References 32 publications

Studies on the Immunomodulatory Effects of Lactoferrin in Rats Infected with E. coli

Studies on the Immunomodulatory Effects of Lactoferrin in Rats Infected with E. coli

Lactoferrin - A Novel Bone Growth Factor

Dietary immunostimulants influence specific immune response and resistance of healthy and immunocompromised Asian catfish Clarias batrachus to Aeromonas hydrophila infection

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