Dietary cadmium and benzo(a)pyrene increased intestinal metallothionein expression in the fish Fundulus heteroclitus

Roesijadi, G.; Rezvankhah, Saeid; Pérez‐Matus, Alejandro; Mitelberg, A.; Torruellas, K.; Veld, Peter A. Van

doi:10.1016/j.marenvres.2008.10.002

Cited by 15 publications

(5 citation statements)

References 32 publications

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“…Moreover, they evaluated developmental deformities by “visual inspection” at 36 hpf at which time point development (organogenesis) is still occurring. Other effects of dietary BaP exposures to fish have been investigated (Couillard et al, 2009; Roesijadi et al, 2009; Yuen and Au, 2006), but none of these studies investigated offspring development or multigenerational effects.…”

Section: Discussionmentioning

confidence: 99%

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

et al. 2014

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In the aquatic environment, adverse outcomes from dietary polycyclic aromatic hydrocarbon (PAH) exposure are poorly understood, and multigenerational developmental effects following exposure to PAHs are in need of exploration. Benzo[a]pyrene (BaP), a model PAH, is a recognized carcinogen and endocrine disruptor. Here adult zebrafish (F0) were fed 0, 10, 114, or 1012 μg BaP/g diet at a feed rate of 1% body weight twice/day for 21 days. Eggs were collected and embryos (F1) were raised to assess mortality and time to hatch at 24, 32, 48, 56, 72, 80, and 96 hours post fertilization (hpf) before scoring developmental deformities at 96 hpf. F1 generation fish were raised to produce the F2 generation followed by the F3 and F4 generations. Mortality significantly increased in the higher dose groups of BaP (2.3 and 20 μg BaP/g fish) in the F1 generation while there were no differences in the F2, F3, or F4 generations. In addition, premature hatching was observed among the surviving fish in the higher dose of the F1 generation, but no differences were found in the F2 and F3 generations. While only the adult F0 generation was BaP-treated, this exposure resulted in multigenerational phenotypic impacts on at least two generations (F1 and F2). Body morphology deformities (shape of body, tail, and pectoral fins) were the most severe abnormality observed, and these were most extreme in the F1 generation but still present in the F2 but not F3 generations. Craniofacial structures (length of brain regions, size of optic and otic vesicles, and jaw deformities), although not significantly affected in the F1 generation, emerged as significant deformities in the F2 generation. Future work will attempt to molecularly anchor the persistent multigenerational phenotypic deformities noted in this study caused by BaP exposure.

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Section: Discussionmentioning

confidence: 99%

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

et al. 2014

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“…Such increase in MT mRNA levels after Cd exposure was observed in Crassostrea virginica (Unger and Roesijadi 1993), M. galloprovincialis (Dondero et al 2005), and C. edule (Paul-Pont et al 2010). Several recent studies were interested to the MT mRNA expression as a biomarker of metal pollution instead of the MT/MTLP quantification in bivalves (Unger and Roesijadi 1993;Lemoine et al 2000;Lemoine and Laulier 2003;Dondero et al 2005;Banni et al 2007;LadharChaabouni et al 2008;Choi et al 2008;Bigot et al 2009), fishes (Tom et al 2004;Kim et al 2008;Gao et al 2009;Roesijadi et al 2009), crab (Gao et al 2011) etc., and showed an increase of MT mRNA levels after exposure to metals. Nevertheless, Chang et al (2007) showed a decrease of MT mRNA levels in gills of the clam Meretrix lusoria exposed to 200 and 1,000 μg/l of Cd during 5 days.…”

Section: Mt Induction and Physiological Variabilitymentioning

confidence: 99%

Use of metallothioneins as biomarkers for environmental quality assessment in the Gulf of Gabès (Tunisia)

Ladhar-Chaabouni

Machreki-Ajmi

Hamza-Chaffai

2011

Environ Monit Assess

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Detection and assessment of the impact of pollution on biological resources imply increasing research on early-warning markers such as metallothioneins (MTs) in metal exposure. In this paper, we have collated published information on the use of metallothioneins and metallothionein-like proteins (MTLPs) as biomarkers for environmental quality assessment in the Gulf of Gabès. In this area, some species of fish and bivalve were used as bioindicators of pollution. In these species, an induction of MTs/MTLPs by the essential metals such as Cu and Zn and the non-essential metals such as Cd was observed by different authors who suggest the potential use of these proteins as biomarkers. However, MT concentrations can be influenced by many biotic (sex, maturity stages, and tissues) and abiotic factors (temperature, salinity, and pH). This is essentially the case in field studies where many parameters can randomly affect MT levels, so the endogeneous regulation of MTs must be considered before using MTs as an indicator of heavy metal exposure. Moreover, the use of biomarker cannot be examined independently of the evaluation of techniques that enable its quantification. Therefore, the approach to the use of MTs/MTLP as biomarkers of exposure for an assessment of the physiological status of aquatic organisms is discussed in this paper.

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“…The majority of contaminants rarely occur as single compounds, and are most commonly present in the form of mixtures containing chemicals that either do not interact or mutually interact to exert joint actions. Thus, studies of their combined effect have become increasingly important for predicting toxic impact of contaminants on marine organisms (Maria and Bebianno, 2011;Ragusa et al, 2017;Roesijadi et al, 2009). Of all the major contaminants of coastal ecosystems, metals have received significant attention given their ubiquitous distribution and long term persistence in the sediment and biota.…”

Section: Introductionmentioning

confidence: 99%

The effect of copper and chlorpyrifos co-exposure on biomarkers in the marine mussel Mytilus galloprovincialis

Perić

Burić

2019

Chemosphere

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Metals and organophosphorous pesticides commonly co-occur in marine environment, but the effect of their mixtures on non-target organisms is still poorly understood. This study investigated the combined effect of the essential metal copper (Cu) and organophosphorous pesticide chlorpyrifos (Chp) in mussels Mytilus galloprovincialis after short-term exposure to their sublethal concentrations. Mussels were exposed for four days to 5 and 15 µg l -1 Cu and 0.05 and 5 µg l -1 Chp, and to their binary mixtures. The investigated biomarkers, namely acetylcholinesterase activity (AChE), glutathione S-transferase activity (GST), metallothioneins content (MTs) and lipid peroxide levels (LPO) displayed unspecific and inconsistent response patterns that varied depending on the concentration of chemicals and composition of mixtures. The exposure to Cu or Chp alone did not induce AChE activity changes, whereas only Cu provoked a significant GST activity increase. Exposure to lower and higher concentration of Chp resulted in MTs content and LPO level increase, respectively.Response of biomarkers to mixtures was generally inconsistent. Data integration by IBR index and PCA revealed different stress levels for given exposure conditions, but no explicit differentiation between single and joint exposures was found. The present results showed that low and environmentally relevant concentrations of Cu and Chp in mixtures may result in a detectable biological response, stressing the need for further investigation of joint effects of widespread marine contaminants in sentinel organisms.

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Dietary cadmium and benzo(a)pyrene increased intestinal metallothionein expression in the fish Fundulus heteroclitus

Cited by 15 publications

References 32 publications

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

Use of metallothioneins as biomarkers for environmental quality assessment in the Gulf of Gabès (Tunisia)

The effect of copper and chlorpyrifos co-exposure on biomarkers in the marine mussel Mytilus galloprovincialis

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