Dietary calcium attenuation of body fat gain during high-fat feeding in mice

Parra, Pilar; Bruni, G; Palou, Andreu; Serra, Francisca

doi:10.1016/j.jnutbio.2007.01.009

Cited by 45 publications

(40 citation statements)

References 34 publications

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“…No differences in food intake and in the expression of UCP-1 in BAT or in muscle UCP-3 were found, indicating that lower fat accretion in high-Ca-fed animals was not accompanied by lower energy intake either by activation of BAT or muscle thermogenesis, in accordance with results previously found in normal animals (7,14) but not in a largely characterised transgenic model (15) .…”

Section: Discussionsupporting

confidence: 90%

“…Using a transgenic animal model (over-expressing agouti protein under the control of the aP2 promoter), Zemel and co-workers showed that dietary Ca could influence fat deposition by direct modulation on adipocyte metabolism, increasing thermogenesis and lipolysis and decreasing lipogenesis following a high-Ca diet (5,6) . However, in normal mice, activation of thermogenesis does not seem to be responsible for the lower rate of weight gain seen with a high-fat diet enriched with dairy Ca (7) .Minerals have been suggested to beneficially modulate cardiovascular risk factors (8,9) . However, there are few data available concerning dietary mineral interactions.…”

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confidence: 99%

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Effect of calcium-enriched high-fat diet on calcium, magnesium and zinc retention in mice

Pérez-Gallardo

Gomez

Parra

et al. 2008

BJN

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The aim of this work was to assess the effects of a high-fat diet enriched in Ca, which accompanies lower body fat deposition, on mineral depots, as well as to assess the potential role of adaptive thermogenesis in mice. Male mice were fed ad libitum a high-fat (43 %) diet with a Ca content of 4 g/kg from calcium carbonate (control group) or 12 g/kg (42 % from milk powder and the rest from calcium carbonate) (Ca group) for 56 d. Body weight, food intake and urine were periodically collected. Tissue samples were collected when the mice were killed and the composition was determined. Expression of uncoupling proteins was determined by Western blotting. Mineral content was measured by flame atomic absorption spectrometry. Lower body weight gain and fat accretion was found in the Ca group. This could not be attributable to lower gross energy intake or to activation of adaptive thermogenesis. Although significant urine mineral loss was found in the Ca group, preservation of mineral depots in bone was observed. Our data support the fact that adding more Ca to the diet, using a combination of calcium carbonate plus milk powder containing among other things higher Zn and Mg, contributes to counteracting obesity and improving lipid metabolism.Dietary calcium: Zinc: Magnesium: Obesity Observational and epidemiological data have shown that a low-Ca diet may be a risk factor for obesity development, and beneficial aspects of milk components on metabolic syndrome are gaining strength (1,2) , even though intervention trials have yielded inconsistent results to date (3) .The molecular mechanisms responsible for the impact of dairy products on body weight and fat have been studied in animal models with important contributions from the group of Zemel (4) . Using a transgenic animal model (over-expressing agouti protein under the control of the aP2 promoter), Zemel and co-workers showed that dietary Ca could influence fat deposition by direct modulation on adipocyte metabolism, increasing thermogenesis and lipolysis and decreasing lipogenesis following a high-Ca diet (5,6) . However, in normal mice, activation of thermogenesis does not seem to be responsible for the lower rate of weight gain seen with a high-fat diet enriched with dairy Ca (7) .Minerals have been suggested to beneficially modulate cardiovascular risk factors (8,9) . However, there are few data available concerning dietary mineral interactions. The aim of the present work was to assess the effects of a high-fat diet enriched in Ca on body fat and mineral bone retention, particularly on Ca, Mg and Zn, as well as to assess the potential involvement of adaptive thermogenic mechanisms in mice. Experimental methods Animals and dietsTwelve male mice (C57BL/6J) (Charles River, Spain) weighing approximately 21 g were housed in groups of three and kept in a single metabolic cage throughout the experiment under controlled conditions. After a 1-week adaptation, mice received ad libitum either a control diet or a high-Ca diet (Research Diets Inc., New Brunswick, NJ, USA) for ...

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Section: Discussionsupporting

confidence: 90%

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confidence: 99%

Effect of calcium-enriched high-fat diet on calcium, magnesium and zinc retention in mice

Pérez-Gallardo

Gomez

Parra

et al. 2008

BJN

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“…Age-matched male WT, PXR-KO, and hPXR mice were each randomly separated at weaning (ϳ3 weeks old) into two groups (n ϭ 7-13) for either diet: control diet (12% of calories as fat; catalogue #D03032702) or a HFD (45% of calories from fat; catalogue #D03032705) from Research Diets Inc. (New Brunswick, NJ) for 16 weeks. The detailed diet compositions were previously described except that HFD in this study provided 45% of calories from lard and soybean oil instead of 43% (26). Furthermore, the HFD and control chow diets were matched in the type of fat and other nutrients.…”

Section: Methodsmentioning

confidence: 99%

Role of Pregnane X Receptor in Obesity and Glucose Homeostasis in Male Mice

Spruiell

Richardson

Cullen

et al. 2014

Journal of Biological Chemistry

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Background: PXR is a xenobiotic nuclear receptor that defends against toxic agents. Results: In male mice fed a HFD, the mouse PXR gene promoted obesity, whereas mice lacking the PXR or possessing the human transgene were hyperglycemic. Conclusion: The impact of PXR on HFD-induced obesity and hyperglycemia is species-dependent. Significance: The current data provide in vivo significance of PXR in metabolic syndrome.

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“…High intake of dairy products is related to lower risk of insulin resistance [9] , type two diabetes [10,11] and metabolic syndrome [12][13][14][15] , but the mechanism of action has not been established. The increased intake of dairy products or calcium has also been shown to augment weight loss both in humans and mice [16][17][18][19] . Although part of the effect of dairy products on weight loss can be attributed to calcium, it has been repeatedly demonstrated that the anti-obesity effect of dairy is superior to that of calcium alone [18,20] .…”

Section: Introductionmentioning

confidence: 99%

Metabolomic changes in fatty liver can be modified by dietary protein and calcium during energy restriction

Pilvi¹,

Seppänen‐Laakso²,

Simolin³

et al. 2008

WJG

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AIM:To characterise the effect of energy restriction (ER) on liver lipid and primary metabolite profile by using metabolomic approach. We also investigated whether the effect of energy restriction can be further enhanced by modification of dietary protein source and calcium. METHODS: Liver metabolomic profile of lean and obese C57Bl/6J mice (n = 10/group) were compared with two groups of weight-reduced mice. ER was performed on control diet and whey protein-based high-calcium diet (whey + Ca). The metabolomic analyses were performed using the UPLC/MS based lipidomic platform and the HPLC/MS/MS based primary metabolite platform. RESULTS: ER on both diets significantly reduced hepatic lipid accumulation and lipid droplet size, while only whey + Ca diet significantly decreased blood glucose (P < 0.001) and serum insulin (P < 0.01). In hepatic lipid species the biggest reduction was in the level of triacylglycerols and ceramides while the level of cholesterol esters was significantly increased during ER. Interestingly, diacylglycerol to phospholipid ratio, an indicator of relative amount of diabetogenic diglyceride species, was increased in the control ER group, but decreased in the whey + Ca ER group (P < 0.001, vs obese). ER on whey + Ca diet also totally reversed the obesity induced increase in the relative level of lipotoxic ceramides (P < 0.001, vs obese; P > 0.05, vs lean). These changes were accompanied with up-regulated TCA cycle and pentose phosphate pathway metabolites. CONCLUSION: ER-induced changes on hepatic metabolomic profile can be significantly affected by dietary protein source. The therapeutic potential of whey protein and calcium should be further studied. Pilvi TK, Seppänen-Laakso T, Simolin H, Finckenberg P, Huotari A, Herzig KH, Korpela R, Orešič M, Mervaala EM. Metabolomic changes in fatty liver can be modified by dietary protein and calcium during energy restriction.

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Dietary calcium attenuation of body fat gain during high-fat feeding in mice

Cited by 45 publications

References 34 publications

Effect of calcium-enriched high-fat diet on calcium, magnesium and zinc retention in mice

Effect of calcium-enriched high-fat diet on calcium, magnesium and zinc retention in mice

Role of Pregnane X Receptor in Obesity and Glucose Homeostasis in Male Mice

Metabolomic changes in fatty liver can be modified by dietary protein and calcium during energy restriction

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