Dietary carbohydrate levels and lipid sources modulate the growth performance, fatty acid profiles and intermediary metabolism of blunt snout bream Megalobrama amblycephala in an interactive pattern

Wang, Bingke; Liu, Wenbin; Xu, Chao; Cao, Xiufei; Zhong, Xiao-Qun; Shi, Haojie; Li, Xiangfei

doi:10.1016/j.aquaculture.2017.08.034

Cited by 52 publications

(24 citation statements)

References 55 publications

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“…With the increase of dietary lipid, fatty acid synthesis always being inhibited (Yan et al, ), in this study, the expression of FAS in hepatopancreas also showed the decreased tendency. PK is a critical gene involved in glycolysis to produce pyruvate (Wang et al, ), and in previous research, suitable carbohydrate to lipid ratios would enhance the PK activity of large yellow croaker (Zhou, Wang, Xie, Deng, & Zhou, ), which might indicate L12.85 obtained the optimal carbohydrate to lipid ratio in this study. AMPK is a sensor of energy status; usually, the activation of AMPK indicated the energy was in shortage and energy metabolism was activated (Xu, Yang, Zhao, & Yan, ); in this study, the mRNA expression of AMPK was highest in shrimp fed the L12.85 diet, which indicated that the energy metabolism might be more active in postlarval L. vannamei fed the diet with 128.5 g/kg lipid, while the possible reason was still not clear.…”

Section: Discussionmentioning

confidence: 53%

Optimal dietary lipid requirement of postlarval white shrimp, Litopenaeus vannamei in relation to growth performance, stress tolerance and immune response

et al. 2019

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A 25‐day experiment was conducted to evaluate the optimal lipid level for postlarval Litopenaeus vannamei. Shrimp (1.7 mg) were fed five isonitrogenous diets containing grade levels of lipid (96.6, 114.3, 128.5, 136.5 and 154.5 g/kg diet, respectively). Each diet was assigned to four tanks (500 shrimp), and shrimp were fed six times a day. Weight gain was increased with the increasing dietary lipid levels, and the highest weight gain was observed in shrimp fed diet with 154.5 g/kg lipid (p < 0.05). On the contrary, the survival was lowest in shrimp fed the L15.45 and highest in shrimp fed the L11.43. Triglyceride in hepatopancreas was increased, and cholesterol was decreased with the increasing dietary lipid. Pyruvate kinase and AMPK mRNA expression were highest in shrimp fed the L12.85. Malondialdehyde in whole body was positively correlated with the dietary lipid levels. The mRNA expression of SOD and Caspase 3 was highest in shrimp fed the L12.85. After hypoxia stress, shrimp fed the L12.85 showed highest survival. The mRNA expression of superoxide dismutase and Akirin was highest in shrimp fed the L11.43 and L15.45, respectively. Based on the survival after 25‐day feeding trail and after the hypoxia stress, the optimal dietary lipid for postlarval L. vannamei should be 118–124 g/kg.

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Section: Discussionmentioning

confidence: 53%

Optimal dietary lipid requirement of postlarval white shrimp, Litopenaeus vannamei in relation to growth performance, stress tolerance and immune response

et al. 2019

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“…In this study, whole‐body, liver and muscle lipid contents were positively correlated with dietary lipid levels and the highest value was observed in high‐fat diets. This result may be explained that a mixture of fish oil and soybean oil in high‐fat diets could up‐regulate the activities of lipoprotein lipase and acetyl‐CoA carboxylase in fish, as might consequently promote lipid accumulation (Liang, Ogata, & Oku, ; Wang et al, ). However, the addition of simvastatin down‐regulated significantly liver and muscle lipid contents in high‐fat diet, indicating that dietary simvastatin supplementation might reduce the lipid deposition in tissues.…”

Section: Discussionmentioning

confidence: 99%

Effects of high-fat diets on growth performance, endoplasmic reticulum stress and mitochondrial damage in blunt snout breamMegalobrama amblycephala

et al. 2018

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This study aimed to investigate the relationship between fat deposition and endoplasmic reticulum stress of blunt snout bream through changing dietary lipid levels and adding lipolytic agents. The results showed that high fat diet (HFD) remarkably increased VSI, IPF, whole‐body lipid, tissue lipid contents, plasma levels of LDL‐C, TC and TG contents, cytosolic cytochrome c activity and Ca2+ content and the transcriptions of GRP78, IRE1, XBP1s and SREBP1, whereas the opposite was true for plasma HDL‐C content, SDH and Na+, K+‐ATPase activities in the mitochondria, Ca2+‐ATPase activity in the ER and the transcriptions of XBP1u, PPARα and CPT I. In addition, simvastatin supplementation remarkably decreased VSI, tissue lipid contents, plasma LDL‐C and TC levels, cytosolic cytochrome c activity and Ca2+ content and the transcriptions of GRP78, IRE1, XBP1s and SREBP1, while the opposite was true for plasma HDL‐C level, Ca2+‐ATPase activity and the transcriptions of XBP1u, PPARα and CPT I. Furthermore, the histological results showed that large lipid droplets and ultrastructural impairments of the mitochondria, nucleus and ER were present in hepatocytes of fish fed the HFD, while simvastatin supplementation had the potential to alleviate these damages. Taken together, these results indicated that there was an interaction between lipid deposition and endoplasmic reticulum stress induced by HFD.

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“…In the present study, excess dietary DHA (more than 2.3 g/kg) inhibited SGR and lipid contents of M. amblycephala . The previous study of M. amblycephala found that fish fed fish oil had the lowest SGR than those fed soybean oil and palm oil (Wang et al, ). Glencross and Rutherford () found that high DHA level leads to the increase in plasma urea concentration and fish redness of juvenile barramundi ( Lates calcarifer ).…”

Section: Discussionmentioning

confidence: 92%

“…Besides, DHA sources in diet and life stage of fish are also important reasons. The supplement of fishmeal in the commercial diets of M. amblycephala is generally 30–80 g/kg, the lipid of FM is about 120 g/kg and the content of DHA in fish oil is about 107.69 g/kg (Wang et al, ). It is estimated that the content of DHA in the M. amblycephala commercial diets is approximately 1.0 g/kg, which was consistent with optimum DHA level in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The supplement of fishmeal in the commercial diets of M. amblycephala is generally 30-80 g/kg, the lipid of FM is about 120 g/kg and the content of DHA in fish oil is about 107.69 g/kg (Wang et al, 2017). It is estimated that the con- (Wang et al, 2017). Glencross and Rutherford (2011) found that high DHA level leads to the increase in plasma urea concentration and fish redness of juvenile barramundi (Lates calcarifer).…”

Section: Discussionmentioning

confidence: 99%

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Effects of dietary docosahexaenoic acid on growth performance, fatty acid profile and lipogenesis of blunt snout bream ( Megalobrama amblycephala )

et al. 2019

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Six diets were designed to investigate the effects of dietary docosahexaenoic acid (22:6n‐3; DHA) levels (0.5, 1.3, 2.3, 4.2, 8.1 and 15.9 g/kg diets) on growth performance, fatty acid profile and expression of some lipogenesis‐related genes of blunt snout bream (Megalobrama amblycephala). Fish (average weight: 26.40 ± 0.11 g) were randomly fed one of six diets for 8 weeks. Results indicated that the final body weight (FBW) and specific growth rate (SGR) of fish fed 1.3 g/kg DHA were significantly higher than other groups except for the 2.3 g/kg DHA (p < .05). Compared with other groups, the number of lipid droplet clusters of the liver stained with oil red O in the 2.3 g/kg DHA group was the highest, which was consistent with the lipid contents of whole body and liver. The DHA proportion in liver and muscle significantly increased with the increasing dietary DHA levels (p < .05), which reflected fatty acid profiles of diets. The highest mRNA expressions of acetyl‐CoA carboxylase α (ACCα), fatty acid synthase (FAS) and sterol regulatory element‐binding protein‐1 (SREBP‐1) occurred in the 1.3 g/kg DHA group, followed by 2.3 g/kg DHA. In summary, the supplementation of 1.3–2.3 g/kg DHA could improve growth performance and lipogenesis, and the dietary DHA could improve DHA and PUFA proportion in liver and muscle.

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Dietary carbohydrate levels and lipid sources modulate the growth performance, fatty acid profiles and intermediary metabolism of blunt snout bream Megalobrama amblycephala in an interactive pattern

Cited by 52 publications

References 55 publications

Optimal dietary lipid requirement of postlarval white shrimp, Litopenaeus vannamei in relation to growth performance, stress tolerance and immune response

Optimal dietary lipid requirement of postlarval white shrimp, Litopenaeus vannamei in relation to growth performance, stress tolerance and immune response

Effects of high-fat diets on growth performance, endoplasmic reticulum stress and mitochondrial damage in blunt snout breamMegalobrama amblycephala

Effects of dietary docosahexaenoic acid on growth performance, fatty acid profile and lipogenesis of blunt snout bream ( Megalobrama amblycephala )

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