Dietary cinnamaldehyde nanoemulsion boosts growth and transcriptomes of antioxidant and immune related genes to fight Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus)

El-Hamid, Marwa I. Abd; Ibrahim, Seham M.; Eldemery, Fatma; El-Mandrawy, Shefaa A.M.; Metwally, Aya Sh.; Khalifa, Eman; Elnahriry, Shimaa S.; Ibrahim, Doaa

doi:10.1016/j.fsi.2021.03.021

Cited by 48 publications

(37 citation statements)

References 66 publications

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“…vannamei fed with different dosages of NMC additives compared with the control especially at the treatment of 150 mg NMC/kg diet. In other studies used phytogenic materials, Abd El-Hamid et al ( 2021 ) found no significant ( P > 0.05) differences in serum AST and ALT levels in fish fed diets with different levels of cinnamaldehyde nanoemulsion. Abdel-Latif et al ( 2020 ) reported that activities of serum ALT, AST, and ALP levels were not significantly ( P < 0.05) differed in fish fed with different levels of oregano essential oil as compared to the control group.…”

Section: Discussionmentioning

confidence: 90%

“…The activities of digestive enzymes in fish serum or gut were higher in fish fed with curcumin when compared with the control (Midhun et al 2016 ; Jiang et al 2016 ; Sruthi et al 2018 ). In similar studies, increasing the digestive enzymes activities resulted in improving the growth performance of Nile tilapia fed with graded levels of cinnamon nanoparticles (Abdel-Tawwab et al 2018a ) and cinnamaldehyde nanoemulsion (Abd El-Hamid et al 2021 ).…”

Section: Discussionmentioning

confidence: 93%

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Dietary curcumin nanoparticles promoted the performance, antioxidant activity, and humoral immunity, and modulated the hepatic and intestinal histology of Nile tilapia fingerlings

Abdel‐Tawwab

Eissa

Tawfik

et al. 2022

Fish Physiol Biochem

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The current study investigated the effects of dietary curcumin nanoparticles (C-NPs) on the performance, hemato-biochemical profile, digestive enzymes activities, antioxidant status, humoral immunity, and liver and intestinal histology of Nile tilapia (Oreochromis niloticus). Fish (4.3 ± 0.5 g) were fed with diets enriched with 0.0 (control), 15, 30, 45, and 60 mg C-NPs/kg diet up to apparent satiety thrice a day for 60 days. The growth-stimulating effects of dietary C-NPs were significantly observed in terms of final weight, weight gain %, specific growth rate, and feed intake. Compared with the control group, serum amylase, lipase, and proteases activities of Nile tilapia significantly (P < 0.05) increased alongside the increase in dietary levels of C-NPs in a dose-dependent manner. The counts of red blood cells and white blood cells as well as hemoglobin and hematocrit levels of Nile tilapia fed with 30–60 mg C-NPs/kg diet were statistically (P < 0.05) higher than fish in the control group with no significant differences among them (P > 0.05). Moreover, lymphocytes and monocytes significantly (P > 0.05) increased; meanwhile neutrophils significantly (P > 0.05) decreased as C-NPs levels in diets increased. In a similar trend, antioxidant (malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase) and humoral immunity (lysozyme and total immunoglobulin) biomarkers were significantly higher in C-NPs-fed fish. Liver histology showed improvements in the cell architecture of fish fed with C-NPs containing diets up to 45 mg/kg diet. Compared with the control diet, feeding Nile tilapia with C-NPs diets resulted in a higher villi length/width and absorption area. According to the regression curves, the current study recommends using the dietary C-NP with optimum values of 45–55 mg/kg diet to improve the performance, digestive enzymes, antioxidant activities, and immunity response of Nile tilapia.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 93%

Dietary curcumin nanoparticles promoted the performance, antioxidant activity, and humoral immunity, and modulated the hepatic and intestinal histology of Nile tilapia fingerlings

Abdel‐Tawwab

Eissa

Tawfik

et al. 2022

Fish Physiol Biochem

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“…In particular, phytogenic feed additives or their compounds, i.e., essential oils (EOs) have been displayed to modify gut signaling molecules (5), gut (6) and luminal (7) microbiota composition, gut integrity (8)(9)(10) and pro-and anti-inflammatory cytokines expression (11)(12)(13). Other features of these additives include their capability for increasing the growth performance, digestive functions, or expression of digestive enzyme related genes (14)(15)(16)(17) and to enhance the meat quality in broiler chickens (18)(19)(20). Among many potentially bioactive compounds from plant extracts, eugenol (4-allyl-2-methoxyphenol), a component of clove oil (21), has revealed positive potential impacts on growth performance and intestinal health (22) due to its antioxidant, antibacterial, and anti-inflammatory properties (23).…”

Section: Introductionmentioning

confidence: 99%

Dietary Eugenol Nanoemulsion Potentiated Performance of Broiler Chickens: Orchestration of Digestive Enzymes, Intestinal Barrier Functions and Cytokines Related Gene Expression With a Consequence of Attenuating the Severity of E. coli O78 Infection

et al. 2022

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Recently, the use of essential oils (EOs) or their bioactive compounds encapsulated by nanoparticles as alternative supplements for in-feed antimicrobials is gaining attention, especially in organic poultry production. Focusing on eugenol, its incorporation into the nanoformulation is a novel strategy to improve its stability and bioavailability and thus augment its growth-boosting and antimicrobial activities. Therefore, we explored eugenol nanoemulsion activities in modulating growth, digestive and gut barrier functions, immunity, cecal microbiota, and broilers response to avian pathogenic E. coli challenge (APEC) O78. A total of 1,000 one-day-old broiler chicks were allocated into five groups; negative control (NC, fed basal diet), positive control (PC), and 100, 250, and 400 mg/kg eugenol nanoemulsion supplemented groups. All groups except NC were challenged with APEC O78 at 14 days of age. The results showed that birds fed eugenol nanoemulsion displayed higher BWG, FI, and survivability and most improved FCR over the whole rearing period. Birds fed 400 mg/kg of eugenol nanoemulsion sustained a higher growth rate (24% vs. PC) after infection. Likely, the expression of digestive enzymes' genes (AMY2A, CCK, CELA1, and PNLIP) was more prominently upregulated and unaffected by APEC O78 challenge in the group fed eugenol nanoemulsion at the level of 400 mg/kg. Enhanced gut barrier integrity was sustained post-challenge in the group supplemented with higher levels of eugenol nanoemulsion as evidenced by the overexpression of cathelicidins-2, β-defensin-1, MUC-2, JAM-2, occludin, CLDN-1, and FABP-2 genes. A distinct modulatory effect of dietary eugenol nanoemulsion was observed on cytokine genes (IL-1β, TNF-α, IL-6, IL-8, and IL-10) expression with a prominent reduction in the excessive inflammatory reactions post-challenge. Supplementing eugenol nanoemulsion increased the relative cecal abundance of Lactobacillus species and reduced Enterobacteriaceae and Bacteriods counts. Notably, a prominent reduction in APEC O78 loads with downregulation of papC, iroN, iutA, and iss virulence genes and detrimental modifications in E. coli morphological features were noticed in the 400 mg/kg eugenol nanoemulsion group at the 3rd-week post-challenge. Collectively, we recommend the use of eugenol nanoemulsion as a prospective targeted delivery approach for achieving maximum broilers growth and protection against APEC O78 infection.

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“…Meanwhile, the fact that quercetin can be absorbed in the stomach and small intestine and rapidly metabolize or suffer strong degradation may hinder its access to the colon at desired concentrations to display local antioxidant and anti-inflammatory effects, thus restricting its potential use for IBD management [ 23 ]. Hence, the engineering of innovative dietary formulations targeting nanoparticles (NPs) offers functional food ingredients with various novel features that enhance their bioavailability, quality and safety together with increasing their stability and protecting them from intestinal degradation [ 24 , 25 , 26 , 27 ]. The fate of nanoparticle-based functional ingredients in the gastrointestinal tract greatly varies from that of ingredients that have larger particles owing to their higher surface area with greater motion proficiency, enabling them to easily penetrate the biological barriers, including intestinal epithelial cells [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model

et al. 2022

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Oxidative stress is considered the main etiologic factor involved in inflammatory bowel disease (IBD). Integration of nanocarriers for natural therapeutic agents with antioxidant and anti-inflammatory potential is a novel promising candidate for curing IBD. Herein, the colonic antioxidant and anti-inflammatory effects of different concentrations of quercetin nanoparticles (QT-NPs) were evaluated using a dextran sulfate sodium (DSS)-induced colitis model. Following colitis induction, the efficacy and mechanistic actions of QT-NPs were evaluated by assessing lesion severity, molecular aids controlling oxidative stress and inflammatory response, and histopathological and immunohistochemistry examination of colonic tissues. Administration of QT-NPs, especially at higher concentrations, significantly reduced the disease activity index and values of fecal calprotectin marker compared to the colitic group. Colonic oxidant/antioxidant status (ROS, H2O2, MDA, SOD, CAT, GPX and TAC) was restored after treatment with higher concentrations of QT-NPs. Moreover, QT-NPs at levels of 20 mg/kg and, to a lesser extent, 15 mg/kg reduced Nrf2 and HO-1 gene expression, which was in line with decreasing the expression of iNOS and COX2 in colonic tissues. Higher concentrations of QT-NPs greatly downregulated pro-inflammatory cytokines; upregulated genes encoding occludin, MUC-2 and JAM; and restored the healthy architectures of colonic tissues. Taken together, these data suggest that QT-NPs could be a promising alternative to current IBD treatments.

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Dietary cinnamaldehyde nanoemulsion boosts growth and transcriptomes of antioxidant and immune related genes to fight Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus)

Cited by 48 publications

References 66 publications

Dietary curcumin nanoparticles promoted the performance, antioxidant activity, and humoral immunity, and modulated the hepatic and intestinal histology of Nile tilapia fingerlings

Dietary curcumin nanoparticles promoted the performance, antioxidant activity, and humoral immunity, and modulated the hepatic and intestinal histology of Nile tilapia fingerlings

Dietary Eugenol Nanoemulsion Potentiated Performance of Broiler Chickens: Orchestration of Digestive Enzymes, Intestinal Barrier Functions and Cytokines Related Gene Expression With a Consequence of Attenuating the Severity of E. coli O78 Infection

Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model

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