Dietary Collagen Hydrolysates Ameliorate Furrowed and Parched Skin Caused by Photoaging in Hairless Mice

Abstract

Collagen hydrolysates have been suggested as a favorable antiaging modality in skin photoaged by persistent exposure to ultraviolet radiation (UV). The current study evaluated the beneficial effect of collagen hydrolysates (fsCH) extracted from Pangasius hypophthalmus fish skin on wrinkle formation and moisture preservation in dorsal skin of hairless mice challenged with UV-B. Inter-comparative experiments were conducted for anti-photoaging among fsCH, retinoic acid (RA), N-acetyl-D-glucosamine (NAG), and glycine-proline-hydroxyproline (GPH). Treating human HaCaT keratinocytes with 100−200 μg/mL fsCH reciprocally ameliorated the expression of aquaporin 3 (AQP3) and CD44 deranged by UV-B. The UV-B-induced deep furrows and skin thickening were improved in parched dorsal skin of mice supplemented with 206–412 mg/kg fsCH as well as RA and GPH. The UV-B irradiation enhanced collagen fiber loss in the dorsal dermis, which was attenuated by fsCH through enhancing procollagen conversion to collagen. The matrix metalloproteinase expression by UV-B in dorsal skin was diminished by fsCH, similar to RA and GPH, via blockade of collagen degradation. Supplementing fsCH to UV-B-irradiated mice decreased transepidermal water loss in dorsal skin with reduced AQP3 level and restored keratinocyte expression of filaggrin. The expression of hyaluronic acid synthase 2 and hyaluronidase 1 by UV-B was remarkably ameliorated with increased production of hyaluronic acid by treating fsCH to photoaged mice. Taken together, fsCH attenuated photoaging typical of deep wrinkles, epidermal thickening, and skin water loss, like NAG, RA, or GPH, through inhibiting collagen destruction and epidermal barrier impairment.

Expression analysis of genes important for maintaining skin function in a senescence‐accelerated mouse prone model

“…Reportedly, hyaluronic acid decreases due to ultraviolet irradiation and aging. 38,39 Therefore, this molecule is incorporated into various cosmetics as a countermeasure against age-related loss of skin moisture, loss of elasticity, and increase of wrinkles. Ceramide is responsible for the barrier function of the skin, and the amount of ceramide was reported to be decreased in the skin of atopic dermatitis patients with reduced skin moisture content and disruption of barrier function.…”

“…Reportedly, hyaluronic acid decreases due to ultraviolet irradiation and aging 38,39 . Therefore, this molecule is incorporated into various cosmetics as a countermeasure against age‐related loss of skin moisture, loss of elasticity, and increase of wrinkles.…”

Dietary Collagen Hydrolysates Retard Estrogen Deficiency-Induced Bone Loss through Blocking Osteoclastic Activation and Enhancing Osteoblastic Matrix Mineralization

“…The collagen hydrolysates fsCH were derived from fish skin gelatin, and digested with non-pathogenic Bacillus collagenase-type protease (Amicogen Inc., Jinju, Korea). The preparation and HPLC analysis of fsCH were as previously detailed [ 34 ].…”

“…This study conducted inter-comparative experiments for anti-osteoporotic activity among fsCH, isoflavone, glycine–proline–hydroxyproline (GPH) tripeptide, and calcium lactate. In our previous study [ 34 ], the crude proteins formed ~97% in fsCH, and the contents of collagen tripeptides and GPH were 32.67 ± 0.22% and 3.22 ± 0.02%, respectively. GPH is considered to be a collagen-specific sequence, and is initially hydrolyzed to the dipeptide proline–hydroxyproline (PH) by the epithelium-bound aminopeptidase on the intestinal brush border membrane [ 35 , 36 ].…”

Fish Collagen Peptide (NaticolⓇ) Protects the Skin from Dryness, Wrinkle Formation, and Melanogenesis Both In Vitro and In Vivo

“…Increasing scientific evidence has shown that collagen hydrolysate from fish skin gelatin prevents wrinkle formation, melanogenesis, and skin dryness. We reported previously that collagen or collagen hydrolysate supplementation contributes to good skin health ( Kang et al, 2018 ; Lee et al, 2019 ; Kang et al, 2021 ; Park et al, 2021 ; Kim et al, 2022 ). Previous studies have reported that fish collagen peptide could weaken intestinal inflammation and treat diet-induced obesity and associated disorders ( Astre et al, 2018 ; Rahabi et al, 2022 ).…”