Dietary Conjugated Linoleic Acid Induces Lipolysis in Adipose Tissue of Coconut Oil‐Fed Mice but not Soy Oil‐Fed Mice

Abstract

Mice fed diets containing conjugated linoleic acid (CLA) are leaner than mice not fed CLA. This anti-obesity effect is amplified in mice fed coconut oil-containing or fat free diets, compared to soy oil diets. The present objective was to determine if CLA alters lipolysis in mice fed different base oils. Mice were fed diets containing soy oil (SO), coconut oil (CO), or fat free (FF) for 6 weeks, followed by 10 or 12 days of CLA or no CLA supplementation. Body fat, tissue weights, and ex vivo lipolysis were determined. Relative protein abundance and activation of perilipin, hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL), and adipose differentiation related protein (ADRP) were determined by western blotting. CLA feeding caused mice to have less (P < 0.05) body fat than non-CLA fed mice. This was enhanced in CO and FF-fed mice (CLA × oil source, P < 0.05). There was also a CLA × oil source interaction on lipolysis as CO + CLA and FF + CLA-fed mice had increased (P < 0.05) rates of lipolysis but SO + CLA-fed mice did not. However, after 12 days of CLA consumption, activated perilipin was increased (P < 0.05) only in SO + CLA-fed mice and total HSL and ATGL were decreased (P < 0.05) in CO + CLA-fed mice. Therefore, the enhanced CLA-induced body fat loss in CO and FF-fed mice appears to involve increased lipolysis but this effect may be decreasing by 12 days of CLA consumption.

Effect of Conjugated Linoleic Acids on Nutritional Status and Lipid Metabolism in Rats Fed Linoleic‐Acid‐Deprived Diets

“…In contrast, little effects or lack of them have been observed in rats or other animals models . Concerning the nutritional status, other author have observed a greater tendency to loss of body fat induced by CLA in mice, due to the consumption of diets containing coconut fat or fat free diets . Very little data exists regarding the possible mechanisms by which coconut oil or EFA deprived diets emphasize the antiobesogenic CLA response …”

The effects of coconut oil supplementation on the body composition and lipid profile of rats submitted to physical exercise

“…Also, Ippagunta et al (2011) demonstrated that animals fed with COS have a lower body mass gain when compared to animals fed with a rich diet on long-chain triglycerides like soy oil. The Lee Index is a biomarker very used to identify the obesity state that is equivalent to body mass index (BMI) in humans (WHO 2008, Misra et al 2010).…”

Efectos de los conjugados del ácido linoleico sobre la incorporación tisular de ácidos grasos y metabolismo lipídico en ratas deficientes en ácidos grasos esenciales

“…Es notorio que en el presente trabajo, ni la suplementación con CLA, ni la deficiencia de AGE, modificaron estos parámetros, pero la suplementación con CLA en los animales con deficiencia en AGE condujo a ciertas alteraciones observadas en modelos más sensibles como las descriptas en el ratón, incluyendo reducción del tejido adiposo e incremento del peso hepático. En este sentido, la disminución del peso de los paní-culos adiposos epididimales y retroperitoneales observada por la suplementación con CLA en deficiencia de AGE, probablemente responda a un aumento en la velocidad de oxidación de AG en este tejido inducido conjuntamente por la presencia de CLA y la deficiencia de AGE, como también ocurre en otros modelos experimentales (41,53), y/o a una incrementada lipólisis de TG, como sugiere Ippagunta (54). Estos hallazgos están de acuerdo a los cambios mencionados anteriormente en los niveles de AL y ALA en tejido adiposo, y también es claramente soportado por una mayor β-oxidación descripta por otros autores en tejido adiposo de ratones (10,37), siendo controvertido en ratas (39,52).…”