Dietary Conjugated Linoleic Acid Supplementation Leads to Downregulation of PPAR Transcription in Broiler Chickens and Reduction of Adipocyte Cellularity

Ramiah, Suriya Kumari; Goh, Yong Meng; Wei, Tan Sheau; Yeap, Swee Keong; Ebrahimi, Mahdi

doi:10.1155/2014/137652

Cited by 36 publications

(39 citation statements)

References 62 publications

(62 reference statements)

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“…This is a clear positive relationship between growth performance and abdominal fat accumulation. The fat deposition has been correlated with adipocyte enlargement [10]. Improvement in growth and accumulation of fatness associated with crossing breeding of indigenous chickens with exotic breeds have been documented in several other native crossbreeding programs [11].…”

Section: Impact Of Crossbreeding Of Thai Native Chickens On Growthmentioning

confidence: 98%

Increasing Fat Deposition via Upregulates the Transcription of Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) in Native Crossbred Chicken

Tunim

Phasuk

Aggrey³

et al. 2020

Preprint

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Background: Crossbreeding using exotic breeds is usually employed to improve the growth characteristics of indigenous chickens. This mating not only provides growth but affect adversely to fat deposition as well. We studied the growth, abdominal, subcutaneous and intramuscular fat and mRNA expression of peroxisome proliferator-activated receptor (PPAR) α and PPARγ in adipose and muscle tissues of four chicken breeds [Chee breed (CH) (100% Thai native chicken), Kaimook e-san1 (KM1; 50% CH background), Kaimook e-san2 (KM2; 25% CH background), and broiler (BR)]. This study was aim to study role of PPARs on fat deposition in native crossbred chicken.Results: The BR chickens had higher abdominal fat than other breeds (P<0.05) and the KM2 had an abdominal fat percentage higher than KM1 and CH respectively (P<0.05). The intramuscular fat (IMF) of BR was greater than KM1 and CH (P<0.05). In adipose tissue, PPARα transcription expression was different among the chicken breeds. However, there were breed differences in PPARγ gene expression. Study of abdominal fat PPARγ gene expression showed the BR breed, KM1, and KM2 breed significantly greater (P<0.05) than CH. In 8 to 12 weeks of age, the result shows that the PPARγ expression of the CH breed is less than (P<0.05) KM2. The result of PPARs expression in muscle tissue was similar result in adipose tissue.Conclusion: Crossbreeding improved the growth of the Thai native breed, there was also a corresponding increase in carcass fatness. However, there appears to be a relationship between PPARγ expression and fat deposition traits. therefore, PPARγ activity plays a key role in lipid accumulation by up-regulation.

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Section: Impact Of Crossbreeding Of Thai Native Chickens On Growthmentioning

confidence: 98%

Increasing Fat Deposition via Upregulates the Transcription of Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) in Native Crossbred Chicken

Tunim

Phasuk

Aggrey³

et al. 2020

Preprint

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“…; Ramiah et al . ). Therefore, broiler breeders worldwide have focused on the selection of more efficient lean broiler lines (Decuypere et al .…”

Section: Introductionmentioning

confidence: 97%

“…However, long-term intense selection for fast juvenile growth in broiler chickens has increased their abdominal fat content (AFC) and resulted in metabolic changes (Emmerson 1997;Scheele 1997;Julian 2005). Excessive deposition of abdominal fat has negative impacts on feed efficiency and carcass quality (Demeure et al 2013;Ramiah et al 2014). Therefore, broiler breeders worldwide have focused on the selection of more efficient lean broiler lines (Decuypere et al 2010;Zuidhof et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Selection against abdominal fat percentage may increase intramuscular fat content in broilers

Leng

Zhang

Dong

et al. 2016

J Animal Breeding Genetics

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Excessive abdominal fat content (AFC) has negative impacts on feed efficiency and carcass quality. Unlike AFC, intramuscular fat content (IMFC) could be a favourable trait, which has a positive impact on meat quality. To meet consumers' needs, a long-term goal of broiler breeders is to decrease AFC and improve the IMFC simultaneously. The current study was designed to investigate the relationship between AFC and IMFC and to compare IMFC, including the pectoral major muscle fat content (PIMFC) and intramuscular fat content of leg muscle (LIMFC), between two broiler lines divergently selected for abdominal fat percentage over 17 generations. The results showed that there was a significant difference in PIMFC and LIMFC between the two lines in all five generation populations used. The birds in the lean line had significantly lower AFC but higher PIMFC and LIMFC than the birds in the fat line. We also detected differences in the liver fat content (LFC) between the two lines and the results showed that birds in the fat line had significant higher LFC than birds in the lean line. Our results indicated that a desirable broiler line with higher IMFC but lower AFC could be obtained by genetic selection.

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“…The selection for rapid growth in meat-type chickens has been accompanied by increased abdominal fat deposition [1]. Excessive abdominal fat deposition can decrease feed efficiency and carcass quality, leading to consumer rejection of the meat [2-4]. The excessive deposition of abdominal fat is mainly due to the excessive proliferation and differentiation of adipocytes in adipose tissues.…”

Section: Introductionmentioning

confidence: 99%

Retinoblastoma 1 (RB1) modulates the proliferation of chicken preadipocytes

Wang

et al. 2018

Preprint

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18Retinoblastoma 1 (RB1) has been extensively studied in mammalian species, but its 19 function in avian species is unclear. The objective of this study was to reveal the role of 20 chicken RB1 (Gallus gallus RB1, gRB1) in the proliferation of preadipocytes. In the current 21 study, quantitative real-time PCR analysis showed that the expression levels of gRB1 22 transiently increased during the proliferation of preadipocytes. The MTT assay showed that 23 gRB1 overexpression suppressed preadipocyte proliferation, and gRB1 interference promoted 24 preadipocyte proliferation. Additionally, cell-cycle analysis indicated that gRB1 may play a 25 crucial role in the G1/S transition. Consistently, gene expression analysis showed that gRB1 26 knockdown promoted marker of proliferation Ki-67 (MKi67) expression at 96 h (P < 0.05), 27 and that overexpression of gRB1 reduced MKi67 expression at 72 h (P < 0.05). Together, our 28 study demonstrated that gRB1 inhibited preadipocyte proliferation at least in part by 29 inhibiting the G1 to S phase transition. 30 31 32The selection for rapid growth in meat-type chickens has been accompanied by 36 increased abdominal fat deposition [1]. Excessive abdominal fat deposition can decrease feed 37 efficiency and carcass quality, leading to consumer rejection of the meat [2][3][4]. The excessive 38 deposition of abdominal fat is mainly due to the excessive proliferation and differentiation of 39 adipocytes in adipose tissues. Clarifying the genetic mechanisms of proliferation and 40 differentiation of adipocytes will help control the excessive accumulation of abdominal fat. 41In our previous study, we mapped a quantitative trait locus (QTL) with major effects on 42 abdominal fat trait into a 3.7-Mbp (172.6-176.3 Mbp) region in chicken chromosome 1 using 43 an F 2 population of a broiler × layer cross [5]. In this 3.7-Mbp region, five genes, including 44 Retinoblastoma 1 (RB1), were detected [6]. In the examination of genome-wide selection 45 signatures of abdominal fat content with the chicken 60 k SNP chip and the extended 46 haplotype homozygosity (EHH) assay, a number of genes in the significant core regions were 47 detected, and the RB1 gene was detected once again [7]. In addition, polymorphism analysis 48 of the RB1 gene of Northeast Agricultural University broiler lines divergently selected for 49 abdominal fat content (NEAUHLF) indicated that the polymorphisms of RB1 were associated 50 with abdominal fat content (unpublished data). These results suggest that RB1 plays an 51 important role in the deposition of abdominal fat in broiler chickens. However, the mode of 52 action of RB1 in chicken abdominal fat deposition remains unknown, hence, the aim of this 53 study was to analyze the function of RB1 in the proliferation of chicken preadipocytes.54 Materials and methods 55 Ethics statement 56 All animal work was conducted according to the guidelines for the Care and Use of 57 Experimental Animals established by the Ministry of Science and Technology of the People's 4 58 Rep...

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Dietary Conjugated Linoleic Acid Supplementation Leads to Downregulation of PPAR Transcription in Broiler Chickens and Reduction of Adipocyte Cellularity

Cited by 36 publications

References 62 publications

Increasing Fat Deposition via Upregulates the Transcription of Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) in Native Crossbred Chicken

Increasing Fat Deposition via Upregulates the Transcription of Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) in Native Crossbred Chicken

Selection against abdominal fat percentage may increase intramuscular fat content in broilers

Retinoblastoma 1 (RB1) modulates the proliferation of chicken preadipocytes

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