Dietary Copper Improves Intestinal Morphology via Modulating Intestinal Stem Cell Activity in Pigs

Yin, Lanmei; Yang, Qing; Zhang, Yiming; Wan, Dan; Yin, Yuebang; Wang, Qiye; Huang, Jing; Li, Jianzhong; Yang, Huansheng; Yin, Yulong

doi:10.3390/ani11092513

Cited by 8 publications

(10 citation statements)

References 43 publications

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“…Adult intestinal stem cells respond to environmental cues (including dietary signals) and influence intestinal epithelium homeostasis through the alteration of its function and activity. 40,42,46,47 In recent years, phenolic acids, especially CGA, have gained substantial attention because of their potential benefits for alleviating health-related problems. Chlorogenic acid has been reported in vegetables and fruits but the most commonly studied sources are coffee and tea.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in intestinal morphology are usually accompanied by the process of epithelial cell renewal. 42,52 Intestinal epithelial cells are regenerated continuously throughout adulthood from stem cells located in the base of the intestinal crypt. Previous studies identified Lgr5 as an adult stem-cell marker in the intestine.…”

Section: Discussionmentioning

confidence: 99%

“…identified fatty acid components or their derivatives in high‐fat diets enhance intestinal stem‐cell function. Dietary walnut protects against obesity‐driven intestinal stem‐cell decline, and dietary copper can improve intestinal morphology by modulating intestinal stem‐cell activity in pigs 41,42 . Intestinal stem cells have attracted increasing attention as potential targets for the dietary regulation of gut health.…”

Section: Introductionmentioning

confidence: 99%

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Chlorogenic acid improves intestinal morphology by enhancing intestinal stem‐cell activity

et al. 2023

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BACKGROUND: Chlorogenic acid (CGA), as one of the most abundant naturally occurring phenolic acids, has been documented to be beneficial for intestinal health. However, the underlying mechanism is still not fully understood. The adult intestinal stem cell is the critical driver of epithelial homeostasis and regeneration.RESULTS: This study hypothesized that CGA exerted intestinal health effects by modulating intestinal stem-cell functions. Lgr5-EGFP mice were treated for 14 days, and intestinal organoids derived from these mice were treated for 3 days, using CGA solution. In comparison with the control group, CGA treatment increased intestinal villous height and crypt depth in mice and augmented the area expansion and the number of budding intestinal organoids. Quantitative polymerase chain reaction (qPCR) analysis revealed that CGA treatment significantly increased the expression of genes coding intestinal stem-cell markers in intestinal tissue and organoids, and upregulated the expression of genes coding secretory cell lineages and enterocytes, although not statistically significantly. Fluorescence-activated cell-sorting analysis further confirmed that CGA augmented the number of stem cells. 5-Ethynyl-2'-deoxyuridine (EdU) incorporation and Ki67 immunostaining results also demonstrated that CGA treatment enhanced intestinal stem-cell proliferation.CONCLUSION: Altogether, our findings indicate that CGA could activate intestinal stem-cell and epithelial regeneration, which could contribute to the improvement of intestinal morphology or organoid growth of mice. This highlights a promising mechanism for CGA as an excellent candidate for the formulation of dietary supplements and functional foods for intestinal protection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chlorogenic acid improves intestinal morphology by enhancing intestinal stem‐cell activity

et al. 2023

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“…The intestinal tract is an important organ, which is mainly responsible for digestion and absorption (19). Villus height and crypt depth are commonly used to evaluate the functional status of the small intestine (20). The crypt is considered to be a producer of villus cells, and the lower depth of crypt indicates that the intestinal villi are in good condition (21).…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary L-Theanine on Growth Performance, Antioxidation, Meat Quality, and Intestinal Microflora in White Feather Broilers With Acute Oxidative Stress

et al. 2022

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In order to reduce the negative effects caused by oxidative stress on broilers, it is particularly important to find ways to alleviate oxidative stress. As a natural plant extract, L-theanine has a variety of biological effects, such as improving antioxidant capacity, promoting growth, and enhancing immunity and antitumor. This trial evaluated the effects of dietary supplementation of L-theanine on growth performance, antioxidation, meat quality, and intestinal microflora in 817 White Feather Broilers. A total of 108 21-day-old 817 broilers with similar body weight (BW) were randomly divided into three groups with six replicates per group and six chickens within each replicate. The three groups were corn-soybean-based diet (NC group); basal diet plus drinking water with 30 mg hydrocortisone/kg (PC group); and basal diet supplemented with 400 mg L-theanine/kg plus drinking water with 30 mg hydrocortisone/kg (LT group). Compared with the NC group, from 21 to 24 days of age, the PC and LT groups had decreased BW, average daily gain (ADG), and average daily feed intake (ADFI), and increased feed to gain ratio (F/G; p < 0.05). At 24 days of age, the LT group had improved superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in serum as compared to the NC group (p < 0.05). The LT group broilers also had significantly higher concentrations of malondialdehyde (MDA) in serum and liver (p < 0.05). On the 42nd days, the PC group had lower PH45min (p < 0.05) than the NC and LT groups and higher cooking loss and shear force (p < 0.05). Moreover, the villi height of the PC group was significantly lower in jejunum than the NC group (p < 0.05). The LT group had a higher ZO-1 content in duodenum than the NC and PC groups (p < 0.05). The activity of GSH-Px in the liver of the LT group was increased than in the PC group (p < 0.05). The relative abundance of Firmicutes in the LT group was significantly higher than in the NC and PC groups (p < 0.05). These results suggested that the effects of acute oxidative stress on growth performance and meat quality of broilers are continuous, and dietary supplementation of L-theanine could improve the growth performance and meat quality, enhance the intestinal mucosal barrier and antioxidant capacity, and improve the composition of the intestinal flora of broilers caused by acute oxidative stress.

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“…The sheep in the CTE treatment had a greater villus height in the duodenum, jejunum and ileum than those sheep in the CON and UTE treatments, indicating that dietary coated trace elements were beneficial to improve the absorption function of small intestinal. Previous studies reported that a single supplement of dietary trace minerals had a positive effect on the gut health of animals, such as Fe ( Zhou et al, 2020 ), Cu ( Yin et al, 2021 ), Zn ( Wang et al, 2018 ) and Se ( Samo et al, 2020 ). Replacing inorganic trace elements with complex organic trace minerals in the diet could increase the villus height in the ileum of weaned piglets ( Wang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of coated and uncoated trace elements on growth performance, apparent digestibility, intestinal development and microbial diversity in growing sheep

et al. 2022

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The suitable supplement pattern affects the digestion and absorption of trace minerals by ruminants. This study aimed to compare the effects of coated and uncoated trace elements on growth performance, apparent digestibility, intestinal development and microbial diversity in growing sheep. Thirty 4-month-old male Yunnan semi-fine wool sheep were randomly assigned to three treatments (n = 10) and fed with following diets: basal diet without adding exogenous trace elements (CON), basal diet plus 400 mg/kg coated trace elements (CTE, the rumen passage rate was 65.87%) and basal diet plus an equal amount of trace elements in uncoated form (UTE). Compared with the CON group, the average daily weight gain and apparent digestibility of crude protein were higher (P < 0.05) in the CTE and UTE groups, while there was no difference between the CTE and UTE groups. The serum levels of selenium, iodine and cobalt were higher (P < 0.05) in the CTE and UTE groups than those in the CON group, the serum levels of selenium and cobalt were higher (P < 0.05) in the CTE group than those in the UTE group. Compared with the CON and UTE groups, the villus height and the ratio of villus height to crypt depth in duodenum and ileum were higher (P < 0.05) in the CTE groups. The addition of trace minerals in diet upregulated most of the relative gene expression of Ocludin, Claudin-1, Claudin-2, ZO-1, and ZO-2 in the duodenum and jejunum and metal ion transporters (FPN1 and ZNT4) in small intestine. The relative abundance of the genera Christensenellaceae R-7 group, Ruminococcus 1, Lachnospiraceae NK3A20 group, and Ruminococcaceae in ileum, and Ruminococcaceae UCG-014 and Lactobacillus in colon was higher in the CTE group that in the CON group. These results indicated that dietary trace mineral addition improved the growth performance and intestinal development, and altered the structure of intestinal bacteria in growing sheep. Compared to uncoated form, offering trace mineral elements to sheep in coated form had a higher absorption efficiency, however, had little effect on improving growth performance of growing sheep.

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Dietary Copper Improves Intestinal Morphology via Modulating Intestinal Stem Cell Activity in Pigs

Cited by 8 publications

References 43 publications

Chlorogenic acid improves intestinal morphology by enhancing intestinal stem‐cell activity

Chlorogenic acid improves intestinal morphology by enhancing intestinal stem‐cell activity

Effects of Dietary L-Theanine on Growth Performance, Antioxidation, Meat Quality, and Intestinal Microflora in White Feather Broilers With Acute Oxidative Stress

Comparison of coated and uncoated trace elements on growth performance, apparent digestibility, intestinal development and microbial diversity in growing sheep

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