Dietary Curcumin Alleviated Aflatoxin B1-Induced Acute Liver Damage in Ducks by Regulating NLRP3–Caspase-1 Signaling Pathways

Abstract

Aflatoxin B1 (AFB1) is a mycotoxin widely distributed in animal feed and human food; it represents a serious threat to human and animal health. This study investigates the mechanism by which dietary curcumin protected liver against acute damage caused by AFB1 administration in ducks. One-day-old male ducks (n = 450) were randomly assigned to three groups, the control group, the AFB1 group, and the AFB1 + curcumin group; the first group were fed with basic diet, while the third group was fed basic diet containing 500 mg/kg curcumin. Ducks in the AFB1 group and AFB1 + curcumin group were challenged with AFB1 at the age of 70 days. The results show that AFB1 administration caused liver damage, increased CYP450 content and AFB1-DNA adducts in the liver, and induced oxidative stress and inflammatory response in the liver. Dietary curcumin significantly inhibited the generation of H2O2 and MDA in liver, activated the Nrf2-ARE signaling pathway, and suppressed the NLRP3–caspase-1 signaling pathway in the liver of ducks. Conclusively, curcumin in diet could protect duck liver against the generation of AFB1-DNA adducts, toxicity, oxidation stress and inflammatory response induced by AFB1 through regulating the NLRP3–caspase-1 signaling pathways, demonstrating that curcumin is a potential feed additive agent to reduce the serious harmful effects of AFB1 on duck breeding.

Application of biosensors in aflatoxins detection in food: a review

“…As the conventional methods of aflatoxin detection have some limitations like being expensive and time consuming, there is a need for some emerging novel methods that are simpler, easier, cheaper more reliable and selective. In this regard, the biosensors for aflatoxins detection are of interest in food safety and control (Jin et al, 2021;Xue et al, 2019;El Sheikha et al, 2018) Biosensors A biosensor is applying a selective detection by the help of a biological recognitive element to discover the chemical combinations with electrical, optical and thermal signals (Li et al, 2021). Biosensors have a bio-recognition element, a transducer and a detector.…”

“…As the conventional methods of aflatoxin detection have some limitations like being expensive and time consuming, there is a need for some emerging novel methods that are simpler, easier, cheaper more reliable and selective. In this regard, the biosensors for aflatoxins detection are of interest in food safety and control (Jin et al , 2021; Xue et al , 2019; El Sheikha et al , 2018)…”

Aflatoxin B1 Toxicity and Protective Effects of Curcumin: Molecular Mechanisms and Clinical Implications

“…Additionally, several clinical studies have shown that curcumin has a strong therapeutic potential for treating a few chronic illnesses, including pulmonary, cancer, neurological, cardiovascular, infectious, neoplastic, psychiatric, and metabolic disorders [ 68 ]. Not surprisingly, several in vitro and animal experimental studies confirmed that curcumin supplementation could offer strong protective effects against AFB1-induced liver injury, mutagenicity, hepatocarcinogenicity, renal dysfunction, ileum damage, immune toxicity via regulating multiple above-mentioned targets [ 26 , 34 , 60 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 ]. The detailed molecular pathways are discussed in the section below.…”

“…Wang et al discovered that oral administration of AFB1 at 750 μg/kg BW for 30 days might produce significant kidney oxidative damage, as demonstrated by the loss of antioxidative enzyme (e.g., CAT and SOD) activities and the decreases in GSH levels and increases in hydrogen peroxide (H 2 O 2 ) and levels of MDA in the kidney tissues; meanwhile, oral administration of curcumin supplementation could significantly inhibit AFB1-induced oxidative stress by upregulating the GSH content and SOD and CAT activities [ 70 ]. Consistently, curcumin supplementation could also significantly improve AFB1-exposure-induced cytotoxicity in bovine fetal hepatocyte-derived cells, renal damage in mice and chickens, and liver, spleen, and ileum damage in ducks by inhibiting the production of ROS and oxidative stress through the upregulation of activities of antioxidant enzymes and levels of antioxidants or free radical scavenging [ 60 , 69 , 70 , 72 , 74 , 75 ]. Very recently, Damiano et al found that curcumin supplementation significantly inhibits the expression of NADPH Oxidase 4 (NOX4) mRNA and protein, thus inhibiting AFB1-induced renal oxidative stress in chicken [ 75 ].…”

Natural Antioxidant By-Product Mixture Counteracts the Effects of Aflatoxin B1 and Ochratoxin A Exposure of Piglets after Weaning: A Proteomic Survey on Liver Microsomal Fraction

“…Two of the most prevalent and well-known mycotoxins are ochratoxin A (OTA) and aflatoxin B1 (AFB1) [5][6][7][8][9][10]. These toxins can contaminate food and feed products, which in turn can lead to a range of adverse effects on animal health and nutrition [11][12][13]. In pigs, the exposure to OTA and AFB1 has been shown to impair feed intake, growth performance, and immunity, as well as increase oxidative stress and the risk of various diseases [14][15][16][17].…”