Dietary emamectin benzoate induces dose-dependent variations in haemato-biochemical and erythrocyte-metric parameters of Oreochromis niloticus (L.)

Das, Ratnapriya; Abraham, Thangapalam Jawahar; Singha, Jasmine; Bardhan, Avishek; Patil, Prasanna Kumar

doi:10.1016/j.aquaculture.2022.738680

Cited by 9 publications

(5 citation statements)

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“…These conditions are also shown in relationship to the spawning period (Pickering, 1986) or stress due to low water temperature (Bennet and Neville, 1975). Similar deviations can also occur in bacterial, viral (Alvarez-Pellitero and Pinto, 1987) and parasitic infections and treatments (Mahajan et al 1979; Silva-Souza et al 1999; Das et al 2022) as observed in present study. A possible in uence of the spawning and/or low temperature, which was shown to cause neutrophilia and lymphocytopenia by other authors, cannot be applied in this present study because the sh infested were neither spawning nor maintained in low temperature.…”

Section: Discussionsupporting

confidence: 87%

Natural infestation of an anchor worm, Lernaea sp. in cage culture of Asian Seabass, Lates calcarifer juveniles and its control using an anti-parasitic drug, emamectin benzoate

et al. 2023

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Parasitic infestations and their control programmes are one among the challenges to be considered the most signi cant in aquaculture. A parasitic infestation was studied elaborately in Asian Seabass, Lates calcarifer juveniles with clinical signs, post-mortem ndings, morphological and molecular identi cations. In addition, those sh were also treated with emamectin benzoate (EMB) @ 50 µg kg -1 of sh body weight (BW) d -1 for 10 consecutive days under the controlled wet lab facility by feeding through the medicated feed at 4% BW. Results showed that the parasitic prevalence, parasitic intensity (PI) and mortality were 45.5%, 8.17 ± 0.15 per sh and 40% over a period of one week in that existing cage culture. The parasite was identi ed as a crustacean bloodsucker, anchor worm Lernaea sp. and EMB was found to be 100% effective with signi cant reduction in PI over a period of 10 days with improved survival rate of 90% against the untreated group. Infested but treated group revealed substantial haematological improvement in parameters such as RBC, WBC, Hb, PCV, large lymphocytes, small lymphocytes and total lymphocytes (P < 0.01). Similarly, comparative histopathology of vital organs also revealed no discernible lesions between the healthy and treated sh juvenile as compared to that of infested untreated group. Hence, EMB can be used to control the Lernaea sp. infestation in Asian Seabass.

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Section: Discussionsupporting

confidence: 87%

Natural infestation of an anchor worm, Lernaea sp. in cage culture of Asian Seabass, Lates calcarifer juveniles and its control using an anti-parasitic drug, emamectin benzoate

et al. 2023

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“…The experiment was carried out in sequentially labelled fibreglass reinforced plastic tanks with 25 farm-grown O. niloticus juveniles having size 13.56 ± 0.46 g. The top-coated EB diets were offered to tilapias at 2% body weight thrice daily for 7 (7D group) and 14 (14D group) consecutive days separately (Das et al, 2022). For a stress-free collection of blood, the clove oil anaesthetized fish were sampled and blood was collected without anticoagulant in 2-mL sterile syringes.…”

Section: Methodsmentioning

confidence: 99%

“…Often, the farmers disregard the recommended dose and dosage of medications to see results quickly, which may affect the health of fish. The haematological responses of freshwater fish under the stress of toxicants may be used as an indicator in biomonitoring programmes to assess fish health (Das et al, 2022). This study compared erythromorphologic alterations of Nile tilapia Oreochromis niloticus fed the recommended and extended dose of EB.…”

Section: Iesfac -2023 Fisheries and Aquaculture: An Ecological Perspe...mentioning

confidence: 99%

IESFAC Proceedings 2023

2023

IJE

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The Indian Ecological Society, Punjab Agricultural University (PAU) is organizing 3 days conference on "Fisheries and Aquaculture: An Ecological Perspective" (IESFAC-2023) from 22 nd to 24 th February, 2023 in collaboration with Guru Angad Dev Veterinary and Animal Sciences University (GADVASU) at Ludhiana, Punjab; which is first of its kind in the State to deliberate on strategic innovations and key issues related to eco-sustainability, economic viability and resilience of aqua food production resources. Socio-economic contribution of fisheries sector is evident from its contributions towards food security, malnutrition, livelihood sustenance, employment generation and economic growth at community, regional, national and global levels; in special reference to rural development in developing countries like India. In view of prospective role of the fisheries sector in fulfilling united nations sustainable development goals (SDGs) to attain "Zero Hunger" and "Good Health and Well Being", it is vital to develop the sector in an eco-responsible manner. Further, conservation and sustainable use of aquatic resources advocated under the SDG-14 "Life below Water" also relates to economic value of fisheries sector in many ways.Veteran experts, scientists, young fisheries professionals, farmers and industry representatives will be sharing the conference platform to present latest innovative technologies, requirements, issues and strategies for sustainable development of the sector; under different theme areas covering aquaculture production systems; sustainable aquafeeds and feed technologies; genetics, breeding and biotechnology, aquaculture environment/health management and one health; responsible aquaculture and climate resilience in fisheries; aquatic ecosystem assessment, management and conservation; processing, value addition, quality assurance and marketing; and capacity building and socio-economic empowerment. National and international experts have contributed their research findings and experiences for wider sharing and debate for fruitful linkages and applications. Keynote and lead speakers from esteemed national and international institutes will interact with the participants on important issues related to human resource requirements; sustainable economic utilization of aquatic resources; species and production system diversifications; organic recycling based integrated aquafarming technologies; quality assurance; economic and smart feed technologies; AMR and responsible aquaculture; processing towards circular bioeconomy; marketing and export; and doubling farmers income through food and ornamental fish/shellfish culture in freshwater, brackish water and marine water resources.An effective forward and backward feedback system is required to address need based regional requirements of the stakeholders to remove weak links within the supply chain and in this context an "Academia-Farmer-Industry" interface during the conference will help in spotlighting required actions to reinforce the sector in interest of stak...

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“…Referred to as shape-shifting erythrocytes, poikilocytosis can result from internal damage, external stress, or genetic abnormalities [9,10]. Fish poikilocytosis is linked to conditions such as viral infections, anemia, and oxidative stress, making it a valuable biomarker in fish health assessment [7,11,12]. Its usefulness has gained attention in aquaculture and fisheries management [2,5].…”

Section: Introductionmentioning

confidence: 99%

“…Piscine poikilocytosis is induced by foreign particles, heavy metal toxicity, pharmaceuticals, and antibiotics, leading to altered erythrocyte morphology and anemic conditions [3,5,[13][14][15][16][17]. Dietary florfenicol and emamectin benzoate cause erythro-morphological alterations in healthy Oreochromis niloticus juveniles [2,3,11,12]. Poikilocytes have diverse characteristics; not all distorted erythrocytes imply poikilocytosis [18].…”

Section: Introductionmentioning

confidence: 99%

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

Bardhan,

Abraham,

Das

et al. 2024

Animal Research and One Health

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Fish health assessment is essential for maintaining sustainable aquatic ecosystems and ensuring the well‐being of wild and farmed fish populations. Hematological parameters are crucial indicators of fish health, with poikilocytosis emerging as a fundamental marker with significant diagnostic value. Poikilocytosis refers to abnormally shaped erythrocytes in bloodstream, reflecting underlying physiological and pathological conditions. Poikilocytosis can occur in various fish species and can be influenced by environmental stressors, infectious agents, nutritional deficiencies, and exposure to pollutants. Morphological alterations in erythrocytes, such as acanthocytes, echinocytes, dacrocytes, schistocytes, spherocytes, and codocytes are common poikilocytes in fish. Understanding the relationship between poikilocytosis and fish health has important implications for disease diagnosis, monitoring, surveillance, and management. By quantifying poikilocytic changes, researchers and veterinarians can differentiate normal variations from pathological conditions, facilitating targeted interventions and treatment strategies. While most studies have focused on heavy metal toxicity, stressors, nutritional deficiencies, pollutants, and therapeutics, the etiological induction of poikilocytosis in fish health has been overlooked. Nonetheless, poikilocytosis remains a valuable biomarker for assessing fish health and their environment. This review highlights piscine poikilocytosis as a significant fish hematological biomarker and its importance in understanding their health and culture conditions.

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Dietary emamectin benzoate induces dose-dependent variations in haemato-biochemical and erythrocyte-metric parameters of Oreochromis niloticus (L.)

Cited by 9 publications

References 30 publications

Natural infestation of an anchor worm, Lernaea sp. in cage culture of Asian Seabass, Lates calcarifer juveniles and its control using an anti-parasitic drug, emamectin benzoate

Natural infestation of an anchor worm, Lernaea sp. in cage culture of Asian Seabass, Lates calcarifer juveniles and its control using an anti-parasitic drug, emamectin benzoate

IESFAC Proceedings 2023

Visualization of poikilocytosis as an emerging erythrocytic biomarker for fish health assessment

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