Dietary exposure to methyl mercury chloride induces alterations in hematology, biochemical parameters, and mRNA expression of antioxidant enzymes and metallothionein in Nile tilapia

Alam, Rasha T. M.; Zeid, Ehsan H. Abu; Khalifa, Bouthaina A.; Arisha, Ahmed Hamed; Reda, Rasha M.

doi:10.1007/s11356-021-13014-5

Cited by 14 publications

(9 citation statements)

References 73 publications

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“…Still, at the same time, it was able to maintain growth within the level of the control one. Our results coincided with previous studies showing a direct relation between Hg 2+ toxicity and fish growth reduction [ 46 ], which might owe to thyroid dysfunction, closely related to metabolism regulation and growth [ 38 , 47 ]. Notably, the high protein content of N. oculata [ 48 ] added to feed in our experiment significantly enhanced the healthy fish’s growth ( p < 0.05).…”

Section: Discussionsupporting

confidence: 92%

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Zahran¹,

Ahmed²,

Hassan³

et al. 2023

Biol Trace Elem Res

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The current study evaluated the potential ameliorative effect of a dietary immune modulator, Nannochloropsis oculata microalga, on the mercuric chloride (HgCl2)-induced toxicity of Nile tilapia. Nile tilapia (45–50 g) were fed a control diet or exposed to ¼ LC50 of HgCl2 (0.3 mg/L) and fed on a medicated feed supplemented with N. oculata (5% and 10% (50 or 100 g/kg dry feed)) for 21 days. Growth and somatic indices, Hg2+ bioaccumulation in muscles, and serum acetylcholinesterase (AChE) activity were investigated. Antioxidant and stress-related gene expression analyses were carried out in gills and intestines. Histopathological examinations of gills and intestines were performed to monitor the traits associated with Hg2+ toxicity or refer to detoxification. Hg2+ toxicity led to significant musculature bioaccumulation, inhibited AChE activity, downregulated genes related to antioxidants and stress, and elicited histopathological changes in the gills and intestine. Supplementation with N. oculata at 10% was able to upregulate the anti-oxidative-related genes while downregulated the stress apoptotic genes in gills and intestines compared to the unexposed group. In addition, minor to no histopathological traits were detected in the gills and intestines of the N. oculata-supplemented diets. Our data showed the benefit of dietary N. oculata in suppressing Hg2+ toxicity, which might support its efficacy as therapeutic/preventive agent to overcome environmental heavy metal pollution in aquatic habitats.

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Section: Discussionsupporting

confidence: 92%

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Zahran¹,

Ahmed²,

Hassan³

et al. 2023

Biol Trace Elem Res

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“…MTR activity can be lowered not only by methylcobalamin shortage but also with inhibiting agents, such as the anesthetic gas N 2 O through irreversible oxidation of cobalamin to Cbl‐III 11 . Other molecules have been identified as inhibitors such as methylmercury, ethanol, or carbon tetrachloride (cleaning agent), which are important to look for in case of acute anemia as in our cases 12,13 …”

Section: Discussionmentioning

confidence: 97%

“…11 Other molecules have been identified as inhibitors such as methylmercury, ethanol, or carbon tetrachloride (cleaning agent), which are important to look for in case of acute anemia as in our cases. 12,13 Regarding the hemolytic component of the anemia, it was demonstrated that oxidative stress can lead to deterioration of macromolecules of red blood cells (RBC) either on the membrane or in the cytoplasm, which ultimately leads to cell damage. 14 In keeping with this, intracytoplasmic homocysteine in RBC could possibly form deleterious aggregates with cytoskeleton proteins between homocysteine's thiol group and sulfhydryl group RBC's proteins that could explain the hemolytic feature as well as abnormal ektacytometry in remethylation disorders during oxidative stress condition and glutathione deficiency.…”

Section: Discussionmentioning

confidence: 99%

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Late‐onset refractory hemolytic anemia in siblings treated for methionine synthase reductase deficiency: A rare complication possibly prevented by hydroxocobalamin dose escalation?

Nguyen,

Deshayes,

Nowoczyn

et al. 2024

JIMD Reports

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Methionine synthase reductase deficiency (cblE) is a rare autosomal recessive inborn error of cobalamin metabolism caused by pathogenic variants in the methionine synthase reductase gene (MTRR). Patients usually exhibit early‐onset bone marrow failure with pancytopenia including megaloblastic anemia. The latter can remain isolated or patients may present developmental delay and rarely macular dysfunction. Treatment mostly includes parenteral hydroxocobalamin to maximize the residual enzyme function and betaine to increase methionine concentrations and decrease homocysteine accumulation. We report herein 2 cblE siblings diagnosed in the neonatal period with isolated pancytopenia who, despite treatment, exhibited in adulthood hemolytic anemia (LDH >11 000 U/L, undetectable haptoglobin, elevated unconjugated bilirubin) which could finally be successfully treated by hydroxocobalamin dose escalation. There was no obvious trigger apart from a parvovirus B19 infection in one of the patients. This is the first report of such complications in adulthood. The use of LDH for disease monitoring could possibly be an additional useful biomarker to adjust hydroxocobalamin dosage. Bone marrow infection with parvovirus B19 can complicate this genetic disease with erythroblastopenia even in the absence of an immunocompromised status, as in other congenital hemolytic anemias. The observation of novel hemolytic features in this rare disease should raise awareness about specific complications in remethylation disorders and plea for hydroxocobalamin dose escalation.

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“…Active fish had a lower number of WBC than less active ones, such as rohu ( Labeo rohita ) [ 56 ]. The higher number of WBC in HG fish fed on barberry root [ 24 ], garlic [ 25 ], ellagic acid [ 57 ], zeolite [ 69 ], butyric acid glycerides [ 59 ], prebiotic inulin [ 48 ] and selenium nanoparticulate [ 70 ] were observed. Conversely, no relation between growth and WBC was observed in different fish species when they were fed on roselle anthocyanin extract [ 60 ], exopolysaccharide [ 27 ] and coriander extract [ 61 ].…”

Section: White Blood Cellsmentioning

confidence: 99%

Blood Performance: A New Formula for Fish Growth and Health

Esmaeili

2021

Biology

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Monitoring fish health in a repeatable and accurate manner can contribute to the profitability and sustainability of aquaculture. Haematological and blood biochemistry parameters have been powerful tools and becoming increasingly common in aquaculture studies. Fish growth is closely related to its health status. A fish with a higher growth rate is more likely to be a healthy one. Any change in the physiological status of the fish, from pollution to nutritional stress, can cause changes in the blood parameters. Various aquaculture studies have measured the following components: red blood cells, white blood cells, haemoglobin, haematocrit, and total protein. However, because these parameters do not always follow the same trend across experimental fish, it is difficult to draw a firm conclusion about which parameter should be considered. Therefore, Blood Performance (BP) as a new formula is introduced, which is a more reliable indicator. This formula is simple and sums up the natural logarithm of the five above-mentioned parameters. More than 90 published peer-reviewed articles that measured these five parameters in the last six years confirmed the reliability and validity of this formula. Regardless of which supplements were added to the diets, the fish with a higher growth rate had higher BP as well. In addition, in 44 studies out of 53 articles, there was a significant positive correlation between specific growth rate and BP. Under different stressful situations, from pollution to thermal stress, the fish under stress had a lower BP than the control. Fish meal and fish oil replacement studies were further evidence for this formula and showed that adding excessive alternative proteins decreased growth along with BP. In conclusion, BP can be a reliable indicator of fish health and growth when it is compared between groups in the same experiment or farm. Although there was a positive correlation between specific growth rate and BP, comparing BP between experiments is not recommended. Standardising the haematological assays can improve the reliability and accuracy of BP across experiments.

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Dietary exposure to methyl mercury chloride induces alterations in hematology, biochemical parameters, and mRNA expression of antioxidant enzymes and metallothionein in Nile tilapia

Cited by 14 publications

References 73 publications

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Toxicity Evaluation, Oxidative, and Immune Responses of Mercury on Nile Tilapia: Modulatory Role of Dietary Nannochloropsis oculata

Late‐onset refractory hemolytic anemia in siblings treated for methionine synthase reductase deficiency: A rare complication possibly prevented by hydroxocobalamin dose escalation?

Blood Performance: A New Formula for Fish Growth and Health

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