Dietary Fat Subgroups, Zinc, and Vegetable Components Are Related to Urine F2a-Isoprostane Concentration, a Measure of Oxidative Stress, in Midlife Women

Abstract

Smoking, diet, and physical activity may impact chronic diseases in part by promoting or attenuating oxidative stress. We evaluated associations between lifestyle factors and urine F(2a)-isoprostanes, a marker of oxidative stress in 1610 participants of the Study of Women's Health Across the Nation (SWAN). Dietary intake and physical activity were assessed at baseline and the 5th year 05 (Y05). These data were related to Y05 urinary F(2a)-isoprostane concentration with regression analyses. Median urine F(2a)-isoprostane concentration was 433 ng/L overall, 917 ng/L in smokers [inter-quartile range (IQR): 467, 1832 ng/L], and 403 ng/L in nonsmokers (IQR: 228, 709 ng/L; P < 0.0001 for difference). Higher trans fat intake was associated with higher urine F(2a)-isoprostane concentration; partial Spearman correlations (rho(x|y)) between Y05 urine F(2a)-isoprostane concentration and trans fatty acids was 0.19 (P = 0.03) in smokers and 0.13 (P < 0.0001) in nonsmokers. Increased log trans fat intake from baseline to Y05 was associated with higher concentration of log urine F(2a)-isoprostanes in nonsmokers (beta = 0.131, SE = 0.04, P = 0.0003). In nonsmokers, the partial correlation (rho(x|y)) between lutein and urine F(2a)-isoprostane concentration was -0.13 (P < 0.0001). Increased intake of log lutein from baseline to Y05 was also associated with lower log urine F(2a)-isoprostane concentration (beta = -0.096, SE = 0.03, P = 0.0005) in nonsmokers. Increased zinc intake from baseline to Y05 was associated with lower log urine F(2a)-isoprostane concentration in smokers and nonsmokers (beta = -0.346, SE = 0.14, P = 0.01), and -0.117, 0.04 (P = 0.001), respectively]. In conclusion, diet (fat subtypes, zinc, and vegetable components) and smoking were associated with urine F(2a)-isoprostanes, a marker of oxidative stress.

Metabolism of trans fatty acid isomers

“…A similar effect on the production of 8-isoprostaglandin F 2α was observed by Kuhnt et al (2006b) after feeding a large quantity of a mixture of 11t-and 12t-18:1 isomers. Association between higher intake of TFA and higher concentration of urine F isoprostanes was also found in the Study of Women's health Across the Nation (SWAN), which included 1610 participants (Tomey et al, 2007).…”

Association of blood lead levels with urinary F2-8α isoprostane and 8-hydroxy-2-deoxy-guanosine concentrations in first-grade Uruguayan children

“…Second, we did not have information on children’s exercise routine or physical activity levels, and could not control for those factors in the analysis. Physical activity may influence oxidative status in the body (Llorente-Cantarero et al, 2012; Tomey et al, 2007) and both inadequate physical activity (Llorente-Cantarero et al, 2012) and acute exercise (Jenkins, 2000) may increase OS. However, we did adjust for other physical measures such as BMI.…”

“…On the other hand, 8-OH-dG is produced by the hydroxylation of the guanosine moiety of a nucleic acid base, and is an established marker of DNA oxidation (Valavanidis et al, 2009). The production of both isoprostane (Holt et al, 2009; Tomey et al, 2007) and 8-OH-dG (Hong et al, 2013; Sram et al, 2012; Engstrom et al, 2010) may be affected by nutrients that modulate the biological pathways for the generation of free radicals or influence the balance between free radicals and antioxidant capacity in the body. We investigated the role of two antioxidants, vitamin C and zinc, for which dietary intake information was available, on the association between BLL and urinary concentrations of isoprostane, and 8-OH-dG in first-grade children.…”

Self-Report of Fruit and Vegetable Intake that Meets the 5 A Day Recommendation Is Associated with Reduced Levels of Oxidative Stress Biomarkers and Increased Levels of Antioxidant Defense in Premenopausal Women

“…No prior studies have focused on young adult women or have looked at the efficacy of the 5 A Day recommendation in terms of oxidative stress, but other investigations have reported an inverse relationship between fruit and vegetable intake and biochemical oxidative stress measures 12, 43, 44 . A cross-sectional analysis of male and female adolescents (mean age: 15 y, n=285) found a substantial inverse association between total fruit and vegetable intake and F 2 -isoprostanes, primarily explained by vitamin C and folate intake (41).…”