Dietary Fatty Acids Alter Lipid Profiles and Induce Myocardial Dysfunction without Causing Metabolic Disorders in Mice

Chen, Bainian; Huang, Yifan; Zheng, Dong; Ni, Rui; Bernards, Mark A.

doi:10.3390/nu10010106

Cited by 11 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, we found upregulation of both n-3 and n-6 PUFA series contents, as well as increased contents of most individual PUFAs in the heart muscle of mice fed an HFD, with the exception of significantly downregulated EPA ( Table 2). This accumulation of specific PUFAs is consistent with previous studies; for example, mitochondrial phospholipids in hearts were shown to readily incorporate dietary DHA [89] or ARA [88]. One possible explanation for the elevated levels of long-chain PUFAs (ARA, DHA, etc.)…”

Section: Discussionsupporting

confidence: 91%

“…Although there are some studies investigating the effects of dietary fat on rodent cardiac functions, most of them do not report specific FA profiles or do so only for total lipid FA profiles in heart tissue [50,53,72]. Alternatively, only FA profiles for one selected group of lipid species are presented [86][87][88]. Some studies focused mostly on SFAs and MUFAs [51], while PUFAs have not been widely reported.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

et al. 2020

View full text Add to dashboard Cite

The Western diet can lead to alterations in cardiac function and increase cardiovascular risk, which can be reproduced in animal models by implementing a high-fat diet (HFD). However, the mechanism of these alterations is not fully understood and may be dependent on alterations in heart lipid composition. The aim of this study was to evaluate the effect of an HFD on the fatty acid (FA) composition of total lipids, as well as of various lipid fractions in the heart, and on heart function. C57BL/6 mice were fed an HFD or standard laboratory diet. The FA composition of chow, serum, heart and skeletal muscle tissues was measured by gas chromatography–mass spectrometry. Cardiac function was evaluated by ultrasonography. Our results showed an unexpected increase in polyunsaturated FAs (PUFAs) and a significant decrease in monounsaturated FAs (MUFAs) in the heart tissue of mice fed the HFD. For comparison, no such effects were observed in skeletal muscle or serum samples. Furthermore, we found that the largest increase in PUFAs was in the sphingolipid fraction, whereas the largest decrease in MUFAs was in the phospholipid and sphingomyelin fractions. The hearts of mice fed an HFD had an increased content of triacylglycerols. Moreover, the HFD treatment altered aortic flow pattern. We did not find significant changes in heart mass or oxidative stress markers between mice fed the HFD and standard diet. The above results suggest that alterations in FA composition in the heart may contribute to deterioration of heart function. A possible mechanism of this phenomenon is the alteration of sphingolipids and phospholipids in the fatty acid profile, which may change the physical properties of these lipids. Since phospho- and sphingolipids are the major components of cell membranes, alterations in their structures in heart cells can result in changes in cell membrane properties.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The intake of HFD has been associated with the development of metabolic diseases and to the onset of neurodegenerative diseases [34][35][36][37][38]. These types of diets harbor PA as one of the main saturated fatty acids [39][40][41]. Although several mechanisms regarding its effects have been characterized in peripheral tissues [10][11][12][13], it remains poorly understood the neuronal responses activated by PA.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiles Reveal Deregulation of Lipid Metabolism and Inflammatory Pathways in Neurons Exposed to Palmitic Acid

et al. 2021

View full text Add to dashboard Cite

The effects of the consumption of high-fat diets (HFD) have been studied to unravel the molecular pathways they are altering in order to understand the link between increased caloric intake, metabolic diseases, and the risk of cognitive dysfunction. The saturated fatty acid, palmitic acid (PA), is the main component of HFD and it has been found increased in the circulation of obese and diabetic people. In the central nervous system, PA has been associated with in ammatory responses in astrocytes, but the effects on neurons exposed to it have not been largely investigated. Given that PA affect a variety of metabolic pathways, we aimed to analyze the transcriptomic pro le activated by this fatty acid to shed light on the mechanisms of neuronal dysfunction. In the current study, we pro led the transcriptome response after PA exposition at non-toxic doses in primary hippocampal neurons. Gene ontology and Reactome pathway analysis revealed a pattern of gene expression which is associated with in ammatory pathways, and importantly, with the activation of lipid metabolism that is considered not very active in neurons. Validation by qRT-PCR of Hmgcs2, Angptl4, Ugt8 and Rnf145 support the results obtained by RNAsEq. Overall, these ndings suggest that neurons are able to respond to saturated fatty acids changing the expression pattern of genes associated with in ammatory response and lipid utilization that may be involved in the neuronal damage associated with metabolic diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptional profiles reveal deregulation of lipid metabolism and inflammatory pathways in neurons exposed to palmitic acid

Leon

Millman

Dominguez

et al. 2021

Preprint

View full text Add to dashboard Cite

The effects of the consumption of high-fat diets (HFD) have been studied to unravel the molecular pathways they are altering in order to understand the link between increased caloric intake, metabolic diseases, and the risk of cognitive dysfunction. The saturated fatty acid, palmitic acid (PA), is the main component of HFD and it has been found increased in the circulation of obese and diabetic people. In the central nervous system, PA has been associated with inflammatory responses in astrocytes, but the effects on neurons exposed to it have not been largely investigated. Given that PA affect a variety of metabolic pathways, we aimed to analyze the transcriptomic profile activated by this fatty acid to shed light on the mechanisms of neuronal dysfunction. In the current study, we profiled the transcriptome response after PA exposition at non-toxic doses in primary hippocampal neurons. Gene ontology and Reactome pathway analysis revealed a pattern of gene expression which is associated with inflammatory pathways, and importantly, with the activation of lipid metabolism that is considered not very active in neurons. Validation by qRT-PCR of Hmgcs2, Angptl4, Ugt8 and Rnf145 support the results obtained by RNAsEq. Overall, these findings suggest that neurons are able to respond to saturated fatty acids changing the expression pattern of genes associated with inflammatory response and lipid utilization that may be involved in the neuronal damage associated with metabolic diseases.

show abstract

Dietary Fatty Acids Alter Lipid Profiles and Induce Myocardial Dysfunction without Causing Metabolic Disorders in Mice

Cited by 11 publications

References 30 publications

The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

The Effect of a High-Fat Diet on the Fatty Acid Composition in the Hearts of Mice

Transcriptional Profiles Reveal Deregulation of Lipid Metabolism and Inflammatory Pathways in Neurons Exposed to Palmitic Acid

Transcriptional profiles reveal deregulation of lipid metabolism and inflammatory pathways in neurons exposed to palmitic acid

Contact Info

Product

Resources

About